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Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins

Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination...

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Autores principales: Libante, Virginie, Sarica, Nazim, Mohamad Ali, Abbas, Gapp, Chloé, Oussalah, Anissa, Guédon, Gérard, Leblond-Bourget, Nathalie, Payot, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563843/
https://www.ncbi.nlm.nih.gov/pubmed/32859088
http://dx.doi.org/10.3390/genes11091004
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author Libante, Virginie
Sarica, Nazim
Mohamad Ali, Abbas
Gapp, Chloé
Oussalah, Anissa
Guédon, Gérard
Leblond-Bourget, Nathalie
Payot, Sophie
author_facet Libante, Virginie
Sarica, Nazim
Mohamad Ali, Abbas
Gapp, Chloé
Oussalah, Anissa
Guédon, Gérard
Leblond-Bourget, Nathalie
Payot, Sophie
author_sort Libante, Virginie
collection PubMed
description Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are integrated into an unrelated IME or in chromosomal sites. After labeling by an antibiotic resistance gene, the conjugative transfer of one of these IMEs (named IME_oriTs) and its host ICE was measured. Although the IME is integrated in an ICE, it does not transfer as a part of the host ICE (no cis-mobilization). The IME excises and transfers separately from the ICE (without impacting its transfer rate) using its own relaxase, distantly related to all known MobT relaxases, and integrates in the oriT of the ICE after transfer. Overall, IME_oriTs use MobT-encoding ICEs both as hosts and as helpers for conjugative transfer. As half of them carry lsa(C), they actively participate in the dissemination of lincosamide–streptogramin A–pleuromutilin resistance among Firmicutes.
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spelling pubmed-75638432020-10-27 Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins Libante, Virginie Sarica, Nazim Mohamad Ali, Abbas Gapp, Chloé Oussalah, Anissa Guédon, Gérard Leblond-Bourget, Nathalie Payot, Sophie Genes (Basel) Article Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are integrated into an unrelated IME or in chromosomal sites. After labeling by an antibiotic resistance gene, the conjugative transfer of one of these IMEs (named IME_oriTs) and its host ICE was measured. Although the IME is integrated in an ICE, it does not transfer as a part of the host ICE (no cis-mobilization). The IME excises and transfers separately from the ICE (without impacting its transfer rate) using its own relaxase, distantly related to all known MobT relaxases, and integrates in the oriT of the ICE after transfer. Overall, IME_oriTs use MobT-encoding ICEs both as hosts and as helpers for conjugative transfer. As half of them carry lsa(C), they actively participate in the dissemination of lincosamide–streptogramin A–pleuromutilin resistance among Firmicutes. MDPI 2020-08-26 /pmc/articles/PMC7563843/ /pubmed/32859088 http://dx.doi.org/10.3390/genes11091004 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Libante, Virginie
Sarica, Nazim
Mohamad Ali, Abbas
Gapp, Chloé
Oussalah, Anissa
Guédon, Gérard
Leblond-Bourget, Nathalie
Payot, Sophie
Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins
title Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins
title_full Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins
title_fullStr Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins
title_full_unstemmed Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins
title_short Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins
title_sort mobilization of imes integrated in the orit of ices involves their own relaxase belonging to the rep-trans family of proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563843/
https://www.ncbi.nlm.nih.gov/pubmed/32859088
http://dx.doi.org/10.3390/genes11091004
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