Cargando…

Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice

Brain inflammation is a key event triggering the pathological process associated with many neurodegenerative diseases. Current personalized medicine and translational research in neurodegenerative diseases focus on adipose-derived stem cells (ASCs), because they are patient-specific, thereby reducin...

Descripción completa

Detalles Bibliográficos
Autores principales: Yatsenko, Kateryna, Lushnikova, Iryna, Ustymenko, Alina, Patseva, Maryna, Govbakh, Iryna, Kyryk, Vitalii, Tsupykov, Oleg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563866/
https://www.ncbi.nlm.nih.gov/pubmed/32707898
http://dx.doi.org/10.3390/jpm10030066
_version_ 1783595584042565632
author Yatsenko, Kateryna
Lushnikova, Iryna
Ustymenko, Alina
Patseva, Maryna
Govbakh, Iryna
Kyryk, Vitalii
Tsupykov, Oleg
author_facet Yatsenko, Kateryna
Lushnikova, Iryna
Ustymenko, Alina
Patseva, Maryna
Govbakh, Iryna
Kyryk, Vitalii
Tsupykov, Oleg
author_sort Yatsenko, Kateryna
collection PubMed
description Brain inflammation is a key event triggering the pathological process associated with many neurodegenerative diseases. Current personalized medicine and translational research in neurodegenerative diseases focus on adipose-derived stem cells (ASCs), because they are patient-specific, thereby reducing the risk of immune rejection. ASCs have been shown to exert a therapeutic effect following transplantation in animal models of neuroinflammation. However, the mechanisms by which transplanted ASCs promote cell survival and/or functional recovery are not fully understood. We investigated the effects of ASCs in in vivo and in vitro lipopolysaccharide (LPS)-induced neuroinflammatory models. Brain damage was evaluated immunohistochemically using specific antibody markers of microglia, astroglia and oligodendrocytes. ASCs were used for intracerebral transplantation, as well as for non-contact co-culture with brain slices. In both in vivo and in vitro models, we found that LPS caused micro- and astroglial activation and oligodendrocyte degradation, whereas the presence of ASCs significantly reduced the damaging effects. It should be noted that the observed ASCs protection in a non-contact co-culture suggested that this effect was due to humoral factors via ASC-released biomodulatory molecules. However, further clinical studies are required to establish the therapeutic mechanisms of ASCs, and optimize their use as a part of a personalized medicine strategy.
format Online
Article
Text
id pubmed-7563866
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75638662020-10-27 Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice Yatsenko, Kateryna Lushnikova, Iryna Ustymenko, Alina Patseva, Maryna Govbakh, Iryna Kyryk, Vitalii Tsupykov, Oleg J Pers Med Article Brain inflammation is a key event triggering the pathological process associated with many neurodegenerative diseases. Current personalized medicine and translational research in neurodegenerative diseases focus on adipose-derived stem cells (ASCs), because they are patient-specific, thereby reducing the risk of immune rejection. ASCs have been shown to exert a therapeutic effect following transplantation in animal models of neuroinflammation. However, the mechanisms by which transplanted ASCs promote cell survival and/or functional recovery are not fully understood. We investigated the effects of ASCs in in vivo and in vitro lipopolysaccharide (LPS)-induced neuroinflammatory models. Brain damage was evaluated immunohistochemically using specific antibody markers of microglia, astroglia and oligodendrocytes. ASCs were used for intracerebral transplantation, as well as for non-contact co-culture with brain slices. In both in vivo and in vitro models, we found that LPS caused micro- and astroglial activation and oligodendrocyte degradation, whereas the presence of ASCs significantly reduced the damaging effects. It should be noted that the observed ASCs protection in a non-contact co-culture suggested that this effect was due to humoral factors via ASC-released biomodulatory molecules. However, further clinical studies are required to establish the therapeutic mechanisms of ASCs, and optimize their use as a part of a personalized medicine strategy. MDPI 2020-07-22 /pmc/articles/PMC7563866/ /pubmed/32707898 http://dx.doi.org/10.3390/jpm10030066 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yatsenko, Kateryna
Lushnikova, Iryna
Ustymenko, Alina
Patseva, Maryna
Govbakh, Iryna
Kyryk, Vitalii
Tsupykov, Oleg
Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice
title Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice
title_full Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice
title_fullStr Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice
title_full_unstemmed Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice
title_short Adipose-Derived Stem Cells Reduce Lipopolysaccharide-Induced Myelin Degradation and Neuroinflammatory Responses of Glial Cells in Mice
title_sort adipose-derived stem cells reduce lipopolysaccharide-induced myelin degradation and neuroinflammatory responses of glial cells in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563866/
https://www.ncbi.nlm.nih.gov/pubmed/32707898
http://dx.doi.org/10.3390/jpm10030066
work_keys_str_mv AT yatsenkokateryna adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice
AT lushnikovairyna adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice
AT ustymenkoalina adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice
AT patsevamaryna adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice
AT govbakhiryna adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice
AT kyrykvitalii adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice
AT tsupykovoleg adiposederivedstemcellsreducelipopolysaccharideinducedmyelindegradationandneuroinflammatoryresponsesofglialcellsinmice