Cargando…

Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids

Obesity is a major health problem in highly industrialized countries. High-fat diet (HFD) is one of the most common causes of obesity and obesity-related disorders. There are considerable differences between fat depots and the corresponding risks of metabolic disorders. We investigated the various e...

Descripción completa

Detalles Bibliográficos
Autores principales: Małodobra-Mazur, Małgorzata, Cierzniak, Aneta, Pawełka, Dorota, Kaliszewski, Krzysztof, Rudnicki, Jerzy, Dobosz, Tadeusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563871/
https://www.ncbi.nlm.nih.gov/pubmed/32962087
http://dx.doi.org/10.3390/genes11091092
_version_ 1783595585210679296
author Małodobra-Mazur, Małgorzata
Cierzniak, Aneta
Pawełka, Dorota
Kaliszewski, Krzysztof
Rudnicki, Jerzy
Dobosz, Tadeusz
author_facet Małodobra-Mazur, Małgorzata
Cierzniak, Aneta
Pawełka, Dorota
Kaliszewski, Krzysztof
Rudnicki, Jerzy
Dobosz, Tadeusz
author_sort Małodobra-Mazur, Małgorzata
collection PubMed
description Obesity is a major health problem in highly industrialized countries. High-fat diet (HFD) is one of the most common causes of obesity and obesity-related disorders. There are considerable differences between fat depots and the corresponding risks of metabolic disorders. We investigated the various effects of an excess of fatty acids (palmitic 16:0, stearic 18:0, and oleic acids 18:1n−9) on adipogenesis of subcutaneous- and visceral-derived mesenchymal stem cells (MSCs) and phenotypes of mature adipocytes. MSCs of white adipose tissue were acquired from adipose tissue biopsies obtained from subcutaneous and visceral fat depots from patients undergoing abdominal surgery. The MSCs were extracted and differentiated in vitro with the addition of fatty acids. Oleic acid stimulated adipogenesis, resulting in higher lipid content and larger adipocytes. Furthermore, oleic acid stimulated adipogenesis by increasing the expression of CCAAT enhancer binding protein β (CEBPB) and peroxisome proliferator activated receptor γ (PPARG). All of the examined fatty acids attenuated the insulin-signaling pathway and radically reduced glucose uptake following insulin stimulation. Visceral adipose tissue was shown to be more prone to generate inflammatory stages. The subcutaneous adipose tissue secreted a greater quantity of adipokines. To summarize, oleic acid showed the strongest effect on adipogenesis. Furthermore, all of the examined fatty acids attenuated insulin signaling and secretion of cytokines and adipokines.
format Online
Article
Text
id pubmed-7563871
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75638712020-10-27 Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids Małodobra-Mazur, Małgorzata Cierzniak, Aneta Pawełka, Dorota Kaliszewski, Krzysztof Rudnicki, Jerzy Dobosz, Tadeusz Genes (Basel) Article Obesity is a major health problem in highly industrialized countries. High-fat diet (HFD) is one of the most common causes of obesity and obesity-related disorders. There are considerable differences between fat depots and the corresponding risks of metabolic disorders. We investigated the various effects of an excess of fatty acids (palmitic 16:0, stearic 18:0, and oleic acids 18:1n−9) on adipogenesis of subcutaneous- and visceral-derived mesenchymal stem cells (MSCs) and phenotypes of mature adipocytes. MSCs of white adipose tissue were acquired from adipose tissue biopsies obtained from subcutaneous and visceral fat depots from patients undergoing abdominal surgery. The MSCs were extracted and differentiated in vitro with the addition of fatty acids. Oleic acid stimulated adipogenesis, resulting in higher lipid content and larger adipocytes. Furthermore, oleic acid stimulated adipogenesis by increasing the expression of CCAAT enhancer binding protein β (CEBPB) and peroxisome proliferator activated receptor γ (PPARG). All of the examined fatty acids attenuated the insulin-signaling pathway and radically reduced glucose uptake following insulin stimulation. Visceral adipose tissue was shown to be more prone to generate inflammatory stages. The subcutaneous adipose tissue secreted a greater quantity of adipokines. To summarize, oleic acid showed the strongest effect on adipogenesis. Furthermore, all of the examined fatty acids attenuated insulin signaling and secretion of cytokines and adipokines. MDPI 2020-09-18 /pmc/articles/PMC7563871/ /pubmed/32962087 http://dx.doi.org/10.3390/genes11091092 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Małodobra-Mazur, Małgorzata
Cierzniak, Aneta
Pawełka, Dorota
Kaliszewski, Krzysztof
Rudnicki, Jerzy
Dobosz, Tadeusz
Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
title Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
title_full Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
title_fullStr Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
title_full_unstemmed Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
title_short Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
title_sort metabolic differences between subcutaneous and visceral adipocytes differentiated with an excess of saturated and monounsaturated fatty acids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563871/
https://www.ncbi.nlm.nih.gov/pubmed/32962087
http://dx.doi.org/10.3390/genes11091092
work_keys_str_mv AT małodobramazurmałgorzata metabolicdifferencesbetweensubcutaneousandvisceraladipocytesdifferentiatedwithanexcessofsaturatedandmonounsaturatedfattyacids
AT cierzniakaneta metabolicdifferencesbetweensubcutaneousandvisceraladipocytesdifferentiatedwithanexcessofsaturatedandmonounsaturatedfattyacids
AT pawełkadorota metabolicdifferencesbetweensubcutaneousandvisceraladipocytesdifferentiatedwithanexcessofsaturatedandmonounsaturatedfattyacids
AT kaliszewskikrzysztof metabolicdifferencesbetweensubcutaneousandvisceraladipocytesdifferentiatedwithanexcessofsaturatedandmonounsaturatedfattyacids
AT rudnickijerzy metabolicdifferencesbetweensubcutaneousandvisceraladipocytesdifferentiatedwithanexcessofsaturatedandmonounsaturatedfattyacids
AT dobosztadeusz metabolicdifferencesbetweensubcutaneousandvisceraladipocytesdifferentiatedwithanexcessofsaturatedandmonounsaturatedfattyacids