Cargando…
LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2
Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an “oncogene,” regulates tumor progression and supports cancer stem cell. However, the tum...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563941/ https://www.ncbi.nlm.nih.gov/pubmed/32638620 http://dx.doi.org/10.1177/0963689720925059 |
_version_ | 1783595601643962368 |
---|---|
author | Hui, Yuanjian Yang, Yan Li, Deping Wang, Juan Di, Maojun Zhang, Shichao Wang, Shasha |
author_facet | Hui, Yuanjian Yang, Yan Li, Deping Wang, Juan Di, Maojun Zhang, Shichao Wang, Shasha |
author_sort | Hui, Yuanjian |
collection | PubMed |
description | Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an “oncogene,” regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism of FEZF1-AS1 on gastric cancer stem cell (GCSC) is yet to be investigated. Here, we discovered that FEZF1-AS1 was upregulated in GC tissues and cell lines. Knockdown of FEZF1-AS1 inhibited sphere formation and decreased expression of stem factors and markers. Moreover, FEZF1-AS1 silence also suppressed cell proliferation, viability, invasion, and migration of GCSCs. MiR-363-3p is used as a target of FEZF1-AS1, because its expression was suppressed by FEZF1-AS1 in GCSCs. FEZF1-AS1 could sponge miR-363-3p and increased the expression of high-mobility group AT-hook 2 (HMGA2). The expression of FEZF1-AS1 and miR-363-3p, as well as that of miR-363-3p and HMGA2, was negatively correlated in GC tissues. Finally, FEZF1-AS1 contributed to promotion of GCSCs progression partially through inhibition of miR-363-3p. Subcutaneous xenotransplanted tumor model revealed that silence of FEZF1-AS1 suppressed in vivo tumorigenic ability of GSCS via downregulation of HMGA2. In general, our findings clarified the critical regulatory role of FEZF1-AS1/miR-363-3p/HMGA2 axis in GCSC progression, providing a potential therapeutic target for GC. |
format | Online Article Text |
id | pubmed-7563941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-75639412020-10-26 LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 Hui, Yuanjian Yang, Yan Li, Deping Wang, Juan Di, Maojun Zhang, Shichao Wang, Shasha Cell Transplant Original Article Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an “oncogene,” regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism of FEZF1-AS1 on gastric cancer stem cell (GCSC) is yet to be investigated. Here, we discovered that FEZF1-AS1 was upregulated in GC tissues and cell lines. Knockdown of FEZF1-AS1 inhibited sphere formation and decreased expression of stem factors and markers. Moreover, FEZF1-AS1 silence also suppressed cell proliferation, viability, invasion, and migration of GCSCs. MiR-363-3p is used as a target of FEZF1-AS1, because its expression was suppressed by FEZF1-AS1 in GCSCs. FEZF1-AS1 could sponge miR-363-3p and increased the expression of high-mobility group AT-hook 2 (HMGA2). The expression of FEZF1-AS1 and miR-363-3p, as well as that of miR-363-3p and HMGA2, was negatively correlated in GC tissues. Finally, FEZF1-AS1 contributed to promotion of GCSCs progression partially through inhibition of miR-363-3p. Subcutaneous xenotransplanted tumor model revealed that silence of FEZF1-AS1 suppressed in vivo tumorigenic ability of GSCS via downregulation of HMGA2. In general, our findings clarified the critical regulatory role of FEZF1-AS1/miR-363-3p/HMGA2 axis in GCSC progression, providing a potential therapeutic target for GC. SAGE Publications 2020-07-08 /pmc/articles/PMC7563941/ /pubmed/32638620 http://dx.doi.org/10.1177/0963689720925059 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Hui, Yuanjian Yang, Yan Li, Deping Wang, Juan Di, Maojun Zhang, Shichao Wang, Shasha LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 |
title | LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 |
title_full | LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 |
title_fullStr | LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 |
title_full_unstemmed | LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 |
title_short | LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2 |
title_sort | lncrna fezf1-as1 modulates cancer stem cell properties of human gastric cancer through mir-363-3p/hmga2 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563941/ https://www.ncbi.nlm.nih.gov/pubmed/32638620 http://dx.doi.org/10.1177/0963689720925059 |
work_keys_str_mv | AT huiyuanjian lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 AT yangyan lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 AT lideping lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 AT wangjuan lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 AT dimaojun lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 AT zhangshichao lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 AT wangshasha lncrnafezf1as1modulatescancerstemcellpropertiesofhumangastriccancerthroughmir3633phmga2 |