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Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports

Cellulose hydrogels are considered useful biocompatible and biodegradable materials. However, as few cellulose-dissolving solvents can be used to prepare cellulose hydrogel microspheres, the use of unmodified cellulose-based hydrogel microspheres for enzyme immobilization remains limited. Here, we p...

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Autores principales: Park, Saerom, Oh, Yujin, Jung, Dahun, Lee, Sang Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563986/
https://www.ncbi.nlm.nih.gov/pubmed/32825173
http://dx.doi.org/10.3390/polym12091869
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author Park, Saerom
Oh, Yujin
Jung, Dahun
Lee, Sang Hyun
author_facet Park, Saerom
Oh, Yujin
Jung, Dahun
Lee, Sang Hyun
author_sort Park, Saerom
collection PubMed
description Cellulose hydrogels are considered useful biocompatible and biodegradable materials. However, as few cellulose-dissolving solvents can be used to prepare cellulose hydrogel microspheres, the use of unmodified cellulose-based hydrogel microspheres for enzyme immobilization remains limited. Here, we prepared cellulose/Fe(2)O(3) hydrogel microspheres as enzyme supports through sol-gel transition using a solvent-in-oil emulsion. Cellulose-dissolving solvents including 1-ethyl-3-methylimidazolium ([Emim][Ac]), an aqueous mixture of NaOH and thiourea, tetrabutylammonium hydroxide, and tetrabutylphosphonium hydroxide were used to prepare regular shaped cellulose/Fe(2)O(3) microspheres. The solvent affected microsphere characteristics like crystallinity, hydrophobicity, surface morphology, size distribution, and swelling properties. The immobilization efficiency of the microspheres for lipase was also significantly influenced by the type of cellulose solvent used. In particular, the lipase immobilized on cellulose/Fe(2)O(3) microspheres prepared using [Emim][Ac] showed the highest protein loading, and its specific activity was 3.1-fold higher than that of free lipase. The immobilized lipase could be simply recovered by a magnet and continuously reused.
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spelling pubmed-75639862020-10-29 Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports Park, Saerom Oh, Yujin Jung, Dahun Lee, Sang Hyun Polymers (Basel) Article Cellulose hydrogels are considered useful biocompatible and biodegradable materials. However, as few cellulose-dissolving solvents can be used to prepare cellulose hydrogel microspheres, the use of unmodified cellulose-based hydrogel microspheres for enzyme immobilization remains limited. Here, we prepared cellulose/Fe(2)O(3) hydrogel microspheres as enzyme supports through sol-gel transition using a solvent-in-oil emulsion. Cellulose-dissolving solvents including 1-ethyl-3-methylimidazolium ([Emim][Ac]), an aqueous mixture of NaOH and thiourea, tetrabutylammonium hydroxide, and tetrabutylphosphonium hydroxide were used to prepare regular shaped cellulose/Fe(2)O(3) microspheres. The solvent affected microsphere characteristics like crystallinity, hydrophobicity, surface morphology, size distribution, and swelling properties. The immobilization efficiency of the microspheres for lipase was also significantly influenced by the type of cellulose solvent used. In particular, the lipase immobilized on cellulose/Fe(2)O(3) microspheres prepared using [Emim][Ac] showed the highest protein loading, and its specific activity was 3.1-fold higher than that of free lipase. The immobilized lipase could be simply recovered by a magnet and continuously reused. MDPI 2020-08-19 /pmc/articles/PMC7563986/ /pubmed/32825173 http://dx.doi.org/10.3390/polym12091869 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Saerom
Oh, Yujin
Jung, Dahun
Lee, Sang Hyun
Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports
title Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports
title_full Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports
title_fullStr Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports
title_full_unstemmed Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports
title_short Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe(2)O(3) Hydrogel Microspheres as Enzyme Supports
title_sort effect of cellulose solvents on the characteristics of cellulose/fe(2)o(3) hydrogel microspheres as enzyme supports
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563986/
https://www.ncbi.nlm.nih.gov/pubmed/32825173
http://dx.doi.org/10.3390/polym12091869
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