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Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome

Background: Blood plasma collected at time of surgery is an excellent source of patient material for investigations into disease aetiology and for the discovery of novel biomarkers. Previous studies on limited sets of proteins and patients have indicated that pre-operative fasting and anaesthesia ca...

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Autores principales: Gyllensten, Ulf, Bosdotter Enroth, Sofia, Stålberg, Karin, Sundfeldt, Karin, Enroth, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564209/
https://www.ncbi.nlm.nih.gov/pubmed/32867270
http://dx.doi.org/10.3390/cancers12092439
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author Gyllensten, Ulf
Bosdotter Enroth, Sofia
Stålberg, Karin
Sundfeldt, Karin
Enroth, Stefan
author_facet Gyllensten, Ulf
Bosdotter Enroth, Sofia
Stålberg, Karin
Sundfeldt, Karin
Enroth, Stefan
author_sort Gyllensten, Ulf
collection PubMed
description Background: Blood plasma collected at time of surgery is an excellent source of patient material for investigations into disease aetiology and for the discovery of novel biomarkers. Previous studies on limited sets of proteins and patients have indicated that pre-operative fasting and anaesthesia can affect protein levels, but this has not been investigated on a larger scale. These effects could produce erroneous results in case-control studies if samples are not carefully matched. Methods: The proximity extension assay (PEA) was used to characterize 983 unique proteins in a total of 327 patients diagnosed with ovarian cancer and 50 age-matched healthy women. The samples were collected either at time of initial diagnosis or before surgery under general anaesthesia. Results: 421 of the investigated proteins (42.8%) showed statistically significant differences in plasma abundance levels comparing samples collected at time of diagnosis or just before surgery under anaesthesia. Conclusions: The abundance levels of the plasma proteome in samples collected before incision, i.e., after short-time fasting and under general anaesthesia differs greatly from levels in samples from awake patients. This emphasizes the need for careful matching of the pre-analytical conditions of samples collected from controls to cases at time of surgery in the discovery as well as clinical use of protein biomarkers.
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spelling pubmed-75642092020-10-26 Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome Gyllensten, Ulf Bosdotter Enroth, Sofia Stålberg, Karin Sundfeldt, Karin Enroth, Stefan Cancers (Basel) Article Background: Blood plasma collected at time of surgery is an excellent source of patient material for investigations into disease aetiology and for the discovery of novel biomarkers. Previous studies on limited sets of proteins and patients have indicated that pre-operative fasting and anaesthesia can affect protein levels, but this has not been investigated on a larger scale. These effects could produce erroneous results in case-control studies if samples are not carefully matched. Methods: The proximity extension assay (PEA) was used to characterize 983 unique proteins in a total of 327 patients diagnosed with ovarian cancer and 50 age-matched healthy women. The samples were collected either at time of initial diagnosis or before surgery under general anaesthesia. Results: 421 of the investigated proteins (42.8%) showed statistically significant differences in plasma abundance levels comparing samples collected at time of diagnosis or just before surgery under anaesthesia. Conclusions: The abundance levels of the plasma proteome in samples collected before incision, i.e., after short-time fasting and under general anaesthesia differs greatly from levels in samples from awake patients. This emphasizes the need for careful matching of the pre-analytical conditions of samples collected from controls to cases at time of surgery in the discovery as well as clinical use of protein biomarkers. MDPI 2020-08-27 /pmc/articles/PMC7564209/ /pubmed/32867270 http://dx.doi.org/10.3390/cancers12092439 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gyllensten, Ulf
Bosdotter Enroth, Sofia
Stålberg, Karin
Sundfeldt, Karin
Enroth, Stefan
Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome
title Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome
title_full Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome
title_fullStr Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome
title_full_unstemmed Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome
title_short Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome
title_sort preoperative fasting and general anaesthesia alter the plasma proteome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564209/
https://www.ncbi.nlm.nih.gov/pubmed/32867270
http://dx.doi.org/10.3390/cancers12092439
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