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Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample
A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Consideri...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564281/ https://www.ncbi.nlm.nih.gov/pubmed/32859006 http://dx.doi.org/10.3390/mps3030061 |
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author | Hasegawa, Yu Otoki, Yurika McClorry, Shannon Coates, Laurynne C. Lombardi, Rachel L. Taha, Ameer Y. Slupsky, Carolyn M. |
author_facet | Hasegawa, Yu Otoki, Yurika McClorry, Shannon Coates, Laurynne C. Lombardi, Rachel L. Taha, Ameer Y. Slupsky, Carolyn M. |
author_sort | Hasegawa, Yu |
collection | PubMed |
description | A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses. |
format | Online Article Text |
id | pubmed-7564281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75642812020-10-26 Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample Hasegawa, Yu Otoki, Yurika McClorry, Shannon Coates, Laurynne C. Lombardi, Rachel L. Taha, Ameer Y. Slupsky, Carolyn M. Methods Protoc Protocol A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses. MDPI 2020-08-26 /pmc/articles/PMC7564281/ /pubmed/32859006 http://dx.doi.org/10.3390/mps3030061 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Hasegawa, Yu Otoki, Yurika McClorry, Shannon Coates, Laurynne C. Lombardi, Rachel L. Taha, Ameer Y. Slupsky, Carolyn M. Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample |
title | Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample |
title_full | Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample |
title_fullStr | Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample |
title_full_unstemmed | Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample |
title_short | Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample |
title_sort | optimization of a method for the simultaneous extraction of polar and non-polar oxylipin metabolites, dna, rna, small rna, and protein from a single small tissue sample |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564281/ https://www.ncbi.nlm.nih.gov/pubmed/32859006 http://dx.doi.org/10.3390/mps3030061 |
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