Evaluation of Organic Wastes as Substrates for Rearing Zophobas morio, Tenebrio molitor, and Acheta domesticus Larvae as Alternative Feed Supplements

SIMPLE SUMMARY: The yellow mealworm, super worm, and house cricket are among the most widely produced insects, with high feed conversion efficiency. However, their nutritional composition and development rate generally vary with environmental conditions. From an economic point of view, rearing conditions such as diet, temperature, and time, and insect performance such as mortality and nutritional value are the most important factors. During their intensive growth period at room temperature, when fed a diet of vegetable waste, garden waste, cattle manure, or horse manure with 10% chicken feed, the worms performed better in terms of survival, but presented lower weight. Moreover, they showed differences in growth intensity with time. The yellow mealworm and super worm presented a relatively high fat concentration. House cricket is specifically high in protein; however, its nutritional composition is affected by the rearing substrate, and it was more sensitive to the low-value substrates. Feeding nutrient-poor diets resulted in a low protein concentration and high fat concentration in the larvae of all three species. ABSTRACT: Studies have focused on identifying combinations of insects and organic waste to optimise bio-conversion. Here, the effects of different diets (10% chicken feed complemented with 90% vegetable waste, garden waste, cattle manure, or horse manure) on growth and survival rates, and nutritional value of Zophobas morio and Tenebrio molitor larvae, and Acheta domesticus were investigated. Compared with chicken feed, organic waste decreased the individual larval weight, although green waste showed fewer negative effects than the manure. The macronutrient concentrations in garden waste were moderate compared with chicken feed, and vegetable waste was the poorest diet in terms of nutrient concentration, as revealed by the principal component analysis (PCA). There was no difference in weight between larvae reared on garden waste and those reared on vegetable waste. Tenebrio molitor and A. domesticus showed the maximum growth rates at 71–101 and 36–66 days of age at 22.5 ± 2.5 °C, respectively. The PCA showed that the protein and fibre concentrations were inversely proportional to fat concentration in the larvae. Acheta domesticus was rich in proteins, whereas Z. morio and T. molitor were rich in fat. Feeding nutrient-poor diets resulted in a lower protein and a higher fat concentration in the larvae.