A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein–protein i...

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Autores principales: Dupree, Emmalyn J., Jayathirtha, Madhuri, Yorkey, Hannah, Mihasan, Marius, Petre, Brindusa Alina, Darie, Costel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564415/
https://www.ncbi.nlm.nih.gov/pubmed/32640657
http://dx.doi.org/10.3390/proteomes8030014
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author Dupree, Emmalyn J.
Jayathirtha, Madhuri
Yorkey, Hannah
Mihasan, Marius
Petre, Brindusa Alina
Darie, Costel C.
author_facet Dupree, Emmalyn J.
Jayathirtha, Madhuri
Yorkey, Hannah
Mihasan, Marius
Petre, Brindusa Alina
Darie, Costel C.
author_sort Dupree, Emmalyn J.
collection PubMed
description Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein–protein interactions. This can be especially beneficial in the clinical setting when studying proteins involved in different diseases and conditions. Here, we aim to describe a bottom-up proteomics workflow from sample preparation to data analysis, including all of its benefits and pitfalls. We also describe potential improvements in this type of proteomics workflow for the future.
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spelling pubmed-75644152020-10-26 A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field Dupree, Emmalyn J. Jayathirtha, Madhuri Yorkey, Hannah Mihasan, Marius Petre, Brindusa Alina Darie, Costel C. Proteomes Review Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein–protein interactions. This can be especially beneficial in the clinical setting when studying proteins involved in different diseases and conditions. Here, we aim to describe a bottom-up proteomics workflow from sample preparation to data analysis, including all of its benefits and pitfalls. We also describe potential improvements in this type of proteomics workflow for the future. MDPI 2020-07-06 /pmc/articles/PMC7564415/ /pubmed/32640657 http://dx.doi.org/10.3390/proteomes8030014 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Dupree, Emmalyn J.
Jayathirtha, Madhuri
Yorkey, Hannah
Mihasan, Marius
Petre, Brindusa Alina
Darie, Costel C.
A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field
title A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field
title_full A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field
title_fullStr A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field
title_full_unstemmed A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field
title_short A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field
title_sort critical review of bottom-up proteomics: the good, the bad, and the future of this field
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564415/
https://www.ncbi.nlm.nih.gov/pubmed/32640657
http://dx.doi.org/10.3390/proteomes8030014
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