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Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement

Background: Systemic sclerosis (SSc) is a connective tissue disorder which key feature is a fibrotic process. The role of Endothelin-1 (ET-1) and T-helper (Th)-1 cells in lung and skin fibrosis is well known, although Th17- and Treg-cells were found to be involved. However, no studies analyzed cytok...

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Autores principales: Nicola, Stefania, Rolla, Giovanni, Bucca, Caterina, Geronazzo, Giada, Ridolfi, Irene, Ferraris, Andrea, Fusaro, Enrico, Peroni, Clara Lisa, Dughera, Luca, Brussino, Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564480/
https://www.ncbi.nlm.nih.gov/pubmed/32947843
http://dx.doi.org/10.3390/cells9092106
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author Nicola, Stefania
Rolla, Giovanni
Bucca, Caterina
Geronazzo, Giada
Ridolfi, Irene
Ferraris, Andrea
Fusaro, Enrico
Peroni, Clara Lisa
Dughera, Luca
Brussino, Luisa
author_facet Nicola, Stefania
Rolla, Giovanni
Bucca, Caterina
Geronazzo, Giada
Ridolfi, Irene
Ferraris, Andrea
Fusaro, Enrico
Peroni, Clara Lisa
Dughera, Luca
Brussino, Luisa
author_sort Nicola, Stefania
collection PubMed
description Background: Systemic sclerosis (SSc) is a connective tissue disorder which key feature is a fibrotic process. The role of Endothelin-1 (ET-1) and T-helper (Th)-1 cells in lung and skin fibrosis is well known, although Th17- and Treg-cells were found to be involved. However, no studies analyzed cytokines expression in gastric-juice of SSc patients. Our study aimed to evaluate proinflammatory and profibrotic cytokines in gastric-juice of SSc patients and to investigate their correlations with esophageal dysmotility. Methods: Patients performed upper-gastrointestinal-endoscopy with gastric-juice collection, esophageal manometry and thoracic CT-scan. GM-CSF, ET-1, Th-1 (IFN-γ, IL-1β, TNF-α, IL-2, IL-6, IL-9), Th-17 (IL-17, IL-21, IL-22, IL-23) and T-reg (IL-10, TGF-β) related cytokines were measured in 29 SSc-patients and 20 healthy-controls. Results: Patients showed significant lower levels of IL-6, IL-17, IL-22 and ET-1 (p < 0.005) compared with controls. Patients with atrophic gastritis presented significant lower levels of IL-2, IL-9, IL-6, TGF-β, GM-CSF, IL-17 and ET-1 (p < 0.005) compared to patients without gastritis. Increased values of IL-2, IL-9, IL-1β, IL-17, ET-1 and GM-CSF (p < 0.005) were observed in patients with esophageal impairment. This is the first report of cytokines measurement in gastric juice of patients with SSc. The high IL-17 concentrations in gastric-juice of scleroderma patients with esophageal dysmotility support the signature of Th-17 cells in scleroderma esophageal fibrosis.
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spelling pubmed-75644802020-10-26 Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement Nicola, Stefania Rolla, Giovanni Bucca, Caterina Geronazzo, Giada Ridolfi, Irene Ferraris, Andrea Fusaro, Enrico Peroni, Clara Lisa Dughera, Luca Brussino, Luisa Cells Article Background: Systemic sclerosis (SSc) is a connective tissue disorder which key feature is a fibrotic process. The role of Endothelin-1 (ET-1) and T-helper (Th)-1 cells in lung and skin fibrosis is well known, although Th17- and Treg-cells were found to be involved. However, no studies analyzed cytokines expression in gastric-juice of SSc patients. Our study aimed to evaluate proinflammatory and profibrotic cytokines in gastric-juice of SSc patients and to investigate their correlations with esophageal dysmotility. Methods: Patients performed upper-gastrointestinal-endoscopy with gastric-juice collection, esophageal manometry and thoracic CT-scan. GM-CSF, ET-1, Th-1 (IFN-γ, IL-1β, TNF-α, IL-2, IL-6, IL-9), Th-17 (IL-17, IL-21, IL-22, IL-23) and T-reg (IL-10, TGF-β) related cytokines were measured in 29 SSc-patients and 20 healthy-controls. Results: Patients showed significant lower levels of IL-6, IL-17, IL-22 and ET-1 (p < 0.005) compared with controls. Patients with atrophic gastritis presented significant lower levels of IL-2, IL-9, IL-6, TGF-β, GM-CSF, IL-17 and ET-1 (p < 0.005) compared to patients without gastritis. Increased values of IL-2, IL-9, IL-1β, IL-17, ET-1 and GM-CSF (p < 0.005) were observed in patients with esophageal impairment. This is the first report of cytokines measurement in gastric juice of patients with SSc. The high IL-17 concentrations in gastric-juice of scleroderma patients with esophageal dysmotility support the signature of Th-17 cells in scleroderma esophageal fibrosis. MDPI 2020-09-16 /pmc/articles/PMC7564480/ /pubmed/32947843 http://dx.doi.org/10.3390/cells9092106 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nicola, Stefania
Rolla, Giovanni
Bucca, Caterina
Geronazzo, Giada
Ridolfi, Irene
Ferraris, Andrea
Fusaro, Enrico
Peroni, Clara Lisa
Dughera, Luca
Brussino, Luisa
Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement
title Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement
title_full Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement
title_fullStr Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement
title_full_unstemmed Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement
title_short Gastric Juice Expression of Th-17 and T-Reg Related Cytokines in Scleroderma Esophageal Involvement
title_sort gastric juice expression of th-17 and t-reg related cytokines in scleroderma esophageal involvement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564480/
https://www.ncbi.nlm.nih.gov/pubmed/32947843
http://dx.doi.org/10.3390/cells9092106
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