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Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage

Pluripotent stem cells are classified as naïve and primed cells, based on their in vitro growth characteristics and potential to differentiate into various types of cells. Human-induced pluripotent stem cells (iPSCs, also known as epiblast stem cells [EpiSCs]) have limited capacity to differentiate...

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Autores principales: Kiyokawa, Yuki, Sato, Masahiro, Noguchi, Hirofumi, Inada, Emi, Iwase, Yoko, Kubota, Naoko, Sawami, Tadashi, Terunuma, Miho, Maeda, Takeyasu, Hayasaki, Haruaki, Saitoh, Issei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564489/
https://www.ncbi.nlm.nih.gov/pubmed/32887316
http://dx.doi.org/10.3390/jcm9092838
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author Kiyokawa, Yuki
Sato, Masahiro
Noguchi, Hirofumi
Inada, Emi
Iwase, Yoko
Kubota, Naoko
Sawami, Tadashi
Terunuma, Miho
Maeda, Takeyasu
Hayasaki, Haruaki
Saitoh, Issei
author_facet Kiyokawa, Yuki
Sato, Masahiro
Noguchi, Hirofumi
Inada, Emi
Iwase, Yoko
Kubota, Naoko
Sawami, Tadashi
Terunuma, Miho
Maeda, Takeyasu
Hayasaki, Haruaki
Saitoh, Issei
author_sort Kiyokawa, Yuki
collection PubMed
description Pluripotent stem cells are classified as naïve and primed cells, based on their in vitro growth characteristics and potential to differentiate into various types of cells. Human-induced pluripotent stem cells (iPSCs, also known as epiblast stem cells [EpiSCs]) have limited capacity to differentiate and are slightly more differentiated than naïve stem cells (NSCs). Although there are several in vitro protocols that allow iPSCs to differentiate into pancreatic lineage, data concerning generation of β-cells from these iPSCs are limited. Based on the pluripotentiality of NSCs, it was hypothesized that NSCs can differentiate into pancreatic β-cells when placed under an appropriate differentiation induction condition. We examined whether NSCs can be efficiently induced to form potentially pancreatic β cells after being subjected to an in vitro protocol. Several colonies resembling in vitro-produced β-cell foci, with β-cell-specific marker expression, were observed when NSC-derived embryoid bodies (EBs) were induced to differentiate into β-cell lineage. Conversely, EpiSC-derived EBs failed to form such foci in vitro. Intrapancreatic grafting of the in vitro-formed β-cell foci into nude mice (BALB/c-nu/nu) generated a cell mass containing insulin-producing cells (IPCs), without noticeable tumorigenesis. These NSCs can be used as a promising resource for curing type 1 diabetes.
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spelling pubmed-75644892020-10-26 Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage Kiyokawa, Yuki Sato, Masahiro Noguchi, Hirofumi Inada, Emi Iwase, Yoko Kubota, Naoko Sawami, Tadashi Terunuma, Miho Maeda, Takeyasu Hayasaki, Haruaki Saitoh, Issei J Clin Med Article Pluripotent stem cells are classified as naïve and primed cells, based on their in vitro growth characteristics and potential to differentiate into various types of cells. Human-induced pluripotent stem cells (iPSCs, also known as epiblast stem cells [EpiSCs]) have limited capacity to differentiate and are slightly more differentiated than naïve stem cells (NSCs). Although there are several in vitro protocols that allow iPSCs to differentiate into pancreatic lineage, data concerning generation of β-cells from these iPSCs are limited. Based on the pluripotentiality of NSCs, it was hypothesized that NSCs can differentiate into pancreatic β-cells when placed under an appropriate differentiation induction condition. We examined whether NSCs can be efficiently induced to form potentially pancreatic β cells after being subjected to an in vitro protocol. Several colonies resembling in vitro-produced β-cell foci, with β-cell-specific marker expression, were observed when NSC-derived embryoid bodies (EBs) were induced to differentiate into β-cell lineage. Conversely, EpiSC-derived EBs failed to form such foci in vitro. Intrapancreatic grafting of the in vitro-formed β-cell foci into nude mice (BALB/c-nu/nu) generated a cell mass containing insulin-producing cells (IPCs), without noticeable tumorigenesis. These NSCs can be used as a promising resource for curing type 1 diabetes. MDPI 2020-09-02 /pmc/articles/PMC7564489/ /pubmed/32887316 http://dx.doi.org/10.3390/jcm9092838 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kiyokawa, Yuki
Sato, Masahiro
Noguchi, Hirofumi
Inada, Emi
Iwase, Yoko
Kubota, Naoko
Sawami, Tadashi
Terunuma, Miho
Maeda, Takeyasu
Hayasaki, Haruaki
Saitoh, Issei
Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
title Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
title_full Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
title_fullStr Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
title_full_unstemmed Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
title_short Drug-Induced Naïve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic β-Cell Lineage
title_sort drug-induced naïve ips cells exhibit better performance than primed ips cells with respect to the ability to differentiate into pancreatic β-cell lineage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564489/
https://www.ncbi.nlm.nih.gov/pubmed/32887316
http://dx.doi.org/10.3390/jcm9092838
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