Temperature-Dependent Functional Response of Harmonia axyridis (Coleoptera: Coccinellidae) on the Eggs of Spodoptera litura (Lepidoptera: Noctuidae) in Laboratory

SIMPLE SUMMARY: Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is a notorious and polyphagous pest of several economically important agricultural crops. It is worldwide in distribution and primarily managed through typical dependence on insecticides, which resulted in health and the environmental challenges and selected for resistance development in S. litura field populations. Resistance caused chemical control failures and S. litura outbreaks around the world. This necessitated development of eco-friendly alternative approaches such as biological control. With this view, current study investigated the functional response of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) at various growth stages (i.e., 1st, 2nd, 3rd and 4th instars, and male and female stages) and temperatures (i.e., 15, 20, 25, 30 and 35 °C) against S. litura eggs to enable the recognition of efficient biocontrol stages that could be utilized to suppress S. litura populations. In our findings, egg consumption depended on the growth stage of the predator as well as temperature. All stages consumed S. litura eggs, but more promising stages with active egg consumption were the 4th instar and adults (male and female) typically at higher temperatures (25–35 °C). We conclude that these stages may be exploited to suppress S. litura populations in fields and greenhouses. ABSTRACT: Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is a major pest of several economically important crops with worldwide distribution. Use of insecticides is the principal strategy for its management, which has subsequently led to insecticide resistance and control failures. Functional response of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) at larval and adult stages was evaluated in this study, using S. litura eggs as the prey at various temperatures varying between 15 and 35 °C. Based on logistic model findings, linear parameters of various predatory stages of H. axyridis at various temperatures were significantly negative, which indicate a type II functional response. The theoretical maximum number (T/T(h)) of eggs consumed increased with increasing temperature across all predatory stages. According to the random predator equation, the coefficients of attack rate increased and that of handling time decreased as the temperature increased. The 4th instar and adult stages were superior candidates for biocontrol of the target prey, typically at higher temperatures. The maximum attack rate (0.546 ± 0.058 h(−1)) and lowest handling time (0.189 ± 0.004 h) were exhibited by the females at 30 and 35 °C, respectively, whereas these parameters were inferior for early instars. These findings clearly depict that the 4th instar and adult predators are efficient egg consumers and can serve as potential suppressors of S. litura field populations. The limitations of the predictions formulated by functional response trials are also discussed.