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Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer

Colorectal cancer (CRC) is a major cause of cancer mortality. Currently used CRC biomarkers provide insufficient sensitivity and specificity; therefore, novel biomarkers are needed to improve the CRC detection. Label-free quantitative proteomics were used to identify and compare glycoproteins, enric...

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Autores principales: Chantaraamporn, Juthamard, Champattanachai, Voraratt, Khongmanee, Amnart, Verathamjamras, Chris, Prasongsook, Naiyarat, Mingkwan, Kanokwan, Luevisadpibul, Virat, Chutipongtanate, Somchai, Svasti, Jisnuson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564939/
https://www.ncbi.nlm.nih.gov/pubmed/32971853
http://dx.doi.org/10.3390/proteomes8030026
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author Chantaraamporn, Juthamard
Champattanachai, Voraratt
Khongmanee, Amnart
Verathamjamras, Chris
Prasongsook, Naiyarat
Mingkwan, Kanokwan
Luevisadpibul, Virat
Chutipongtanate, Somchai
Svasti, Jisnuson
author_facet Chantaraamporn, Juthamard
Champattanachai, Voraratt
Khongmanee, Amnart
Verathamjamras, Chris
Prasongsook, Naiyarat
Mingkwan, Kanokwan
Luevisadpibul, Virat
Chutipongtanate, Somchai
Svasti, Jisnuson
author_sort Chantaraamporn, Juthamard
collection PubMed
description Colorectal cancer (CRC) is a major cause of cancer mortality. Currently used CRC biomarkers provide insufficient sensitivity and specificity; therefore, novel biomarkers are needed to improve the CRC detection. Label-free quantitative proteomics were used to identify and compare glycoproteins, enriched by wheat germ agglutinin, from plasma of CRC patients and age-matched healthy controls. Among 189 identified glycoproteins, the levels of 7 and 15 glycoproteins were significantly altered in the non-metastatic and metastatic CRC groups, respectively. Protein-protein interaction analysis revealed that they were predominantly involved in immune responses, complement pathways, wound healing and coagulation. Of these, the levels of complement C9 (C9) was increased and fibronectin (FN1) was decreased in both CRC states in comparison to those of the healthy controls. Moreover, their levels detected by immunoblotting were validated in another independent cohort and the results were consistent with in the study cohort. Combination of CEA, a commercial CRC biomarker, with C9 and FN1 showed better diagnostic performance. Interestingly, predominant glycoforms associated with acetylneuraminic acid were obviously detected in alpha-2 macroglobulin, haptoglobin, alpha-1-acid glycoprotein 1, and complement C4-A of CRC patient groups. This glycoproteomic approach provides invaluable information of plasma proteome profiles of CRC patients and identification of CRC biomarker candidates.
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spelling pubmed-75649392020-10-26 Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer Chantaraamporn, Juthamard Champattanachai, Voraratt Khongmanee, Amnart Verathamjamras, Chris Prasongsook, Naiyarat Mingkwan, Kanokwan Luevisadpibul, Virat Chutipongtanate, Somchai Svasti, Jisnuson Proteomes Article Colorectal cancer (CRC) is a major cause of cancer mortality. Currently used CRC biomarkers provide insufficient sensitivity and specificity; therefore, novel biomarkers are needed to improve the CRC detection. Label-free quantitative proteomics were used to identify and compare glycoproteins, enriched by wheat germ agglutinin, from plasma of CRC patients and age-matched healthy controls. Among 189 identified glycoproteins, the levels of 7 and 15 glycoproteins were significantly altered in the non-metastatic and metastatic CRC groups, respectively. Protein-protein interaction analysis revealed that they were predominantly involved in immune responses, complement pathways, wound healing and coagulation. Of these, the levels of complement C9 (C9) was increased and fibronectin (FN1) was decreased in both CRC states in comparison to those of the healthy controls. Moreover, their levels detected by immunoblotting were validated in another independent cohort and the results were consistent with in the study cohort. Combination of CEA, a commercial CRC biomarker, with C9 and FN1 showed better diagnostic performance. Interestingly, predominant glycoforms associated with acetylneuraminic acid were obviously detected in alpha-2 macroglobulin, haptoglobin, alpha-1-acid glycoprotein 1, and complement C4-A of CRC patient groups. This glycoproteomic approach provides invaluable information of plasma proteome profiles of CRC patients and identification of CRC biomarker candidates. MDPI 2020-09-22 /pmc/articles/PMC7564939/ /pubmed/32971853 http://dx.doi.org/10.3390/proteomes8030026 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chantaraamporn, Juthamard
Champattanachai, Voraratt
Khongmanee, Amnart
Verathamjamras, Chris
Prasongsook, Naiyarat
Mingkwan, Kanokwan
Luevisadpibul, Virat
Chutipongtanate, Somchai
Svasti, Jisnuson
Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer
title Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer
title_full Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer
title_fullStr Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer
title_full_unstemmed Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer
title_short Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer
title_sort glycoproteomic analysis reveals aberrant expression of complement c9 and fibronectin in the plasma of patients with colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564939/
https://www.ncbi.nlm.nih.gov/pubmed/32971853
http://dx.doi.org/10.3390/proteomes8030026
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