Cargando…

Effect of Novel Antibacterial Composites on Bacterial Biofilms

Continuing cariogenic bacterial growth demineralizing dentine beneath a composite filling is the most common cause of tooth restoration failure. Novel composites with antibacterial polylysine (PLS) (0, 4, 6, or 8 wt%) in its filler phase were therefore produced. Remineralising monocalcium phosphate...

Descripción completa

Detalles Bibliográficos
Autores principales: Yaghmoor, Rayan B., Xia, Wendy, Ashley, Paul, Allan, Elaine, Young, Anne M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564959/
https://www.ncbi.nlm.nih.gov/pubmed/32752201
http://dx.doi.org/10.3390/jfb11030055
_version_ 1783595831060856832
author Yaghmoor, Rayan B.
Xia, Wendy
Ashley, Paul
Allan, Elaine
Young, Anne M.
author_facet Yaghmoor, Rayan B.
Xia, Wendy
Ashley, Paul
Allan, Elaine
Young, Anne M.
author_sort Yaghmoor, Rayan B.
collection PubMed
description Continuing cariogenic bacterial growth demineralizing dentine beneath a composite filling is the most common cause of tooth restoration failure. Novel composites with antibacterial polylysine (PLS) (0, 4, 6, or 8 wt%) in its filler phase were therefore produced. Remineralising monocalcium phosphate was also included at double the PLS weight. Antibacterial studies involved set composite disc placement in 1% sucrose-supplemented broth containing Streptococcus mutans (UA159). Relative surface bacterial biofilm mass (n = 4) after 24 h was determined by crystal violet-binding. Live/dead bacteria and biofilm thickness (n = 3) were assessed using confocal laser scanning microscopy (CLSM). To understand results and model possible in vivo benefits, cumulative PLS release from discs into water (n = 3) was determined by a ninhydrin assay. Results showed biofilm mass and thickness decreased linearly by 28% and 33%, respectively, upon increasing PLS from 0% to 8%. With 4, 6, and 8 wt% PLS, respectively, biofilm dead bacterial percentages and PLS release at 24 h were 20%, 60%, and 80% and 85, 163, and 241 μg/disc. Furthermore, initial PLS release was proportional to the square root of time and levelled after 1, 2, and 3 months at 13%, 28%, and 42%. This suggested diffusion controlled release from water-exposed composite surface layers of 65, 140, and 210 μm thickness, respectively. In conclusion, increasing PLS release initially in any gaps under the restoration to kill residual bacteria or longer-term following composite/tooth interface damage might help prevent recurrent caries.
format Online
Article
Text
id pubmed-7564959
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75649592020-10-26 Effect of Novel Antibacterial Composites on Bacterial Biofilms Yaghmoor, Rayan B. Xia, Wendy Ashley, Paul Allan, Elaine Young, Anne M. J Funct Biomater Article Continuing cariogenic bacterial growth demineralizing dentine beneath a composite filling is the most common cause of tooth restoration failure. Novel composites with antibacterial polylysine (PLS) (0, 4, 6, or 8 wt%) in its filler phase were therefore produced. Remineralising monocalcium phosphate was also included at double the PLS weight. Antibacterial studies involved set composite disc placement in 1% sucrose-supplemented broth containing Streptococcus mutans (UA159). Relative surface bacterial biofilm mass (n = 4) after 24 h was determined by crystal violet-binding. Live/dead bacteria and biofilm thickness (n = 3) were assessed using confocal laser scanning microscopy (CLSM). To understand results and model possible in vivo benefits, cumulative PLS release from discs into water (n = 3) was determined by a ninhydrin assay. Results showed biofilm mass and thickness decreased linearly by 28% and 33%, respectively, upon increasing PLS from 0% to 8%. With 4, 6, and 8 wt% PLS, respectively, biofilm dead bacterial percentages and PLS release at 24 h were 20%, 60%, and 80% and 85, 163, and 241 μg/disc. Furthermore, initial PLS release was proportional to the square root of time and levelled after 1, 2, and 3 months at 13%, 28%, and 42%. This suggested diffusion controlled release from water-exposed composite surface layers of 65, 140, and 210 μm thickness, respectively. In conclusion, increasing PLS release initially in any gaps under the restoration to kill residual bacteria or longer-term following composite/tooth interface damage might help prevent recurrent caries. MDPI 2020-08-01 /pmc/articles/PMC7564959/ /pubmed/32752201 http://dx.doi.org/10.3390/jfb11030055 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yaghmoor, Rayan B.
Xia, Wendy
Ashley, Paul
Allan, Elaine
Young, Anne M.
Effect of Novel Antibacterial Composites on Bacterial Biofilms
title Effect of Novel Antibacterial Composites on Bacterial Biofilms
title_full Effect of Novel Antibacterial Composites on Bacterial Biofilms
title_fullStr Effect of Novel Antibacterial Composites on Bacterial Biofilms
title_full_unstemmed Effect of Novel Antibacterial Composites on Bacterial Biofilms
title_short Effect of Novel Antibacterial Composites on Bacterial Biofilms
title_sort effect of novel antibacterial composites on bacterial biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564959/
https://www.ncbi.nlm.nih.gov/pubmed/32752201
http://dx.doi.org/10.3390/jfb11030055
work_keys_str_mv AT yaghmoorrayanb effectofnovelantibacterialcompositesonbacterialbiofilms
AT xiawendy effectofnovelantibacterialcompositesonbacterialbiofilms
AT ashleypaul effectofnovelantibacterialcompositesonbacterialbiofilms
AT allanelaine effectofnovelantibacterialcompositesonbacterialbiofilms
AT youngannem effectofnovelantibacterialcompositesonbacterialbiofilms