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Effect of Novel Antibacterial Composites on Bacterial Biofilms
Continuing cariogenic bacterial growth demineralizing dentine beneath a composite filling is the most common cause of tooth restoration failure. Novel composites with antibacterial polylysine (PLS) (0, 4, 6, or 8 wt%) in its filler phase were therefore produced. Remineralising monocalcium phosphate...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564959/ https://www.ncbi.nlm.nih.gov/pubmed/32752201 http://dx.doi.org/10.3390/jfb11030055 |
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author | Yaghmoor, Rayan B. Xia, Wendy Ashley, Paul Allan, Elaine Young, Anne M. |
author_facet | Yaghmoor, Rayan B. Xia, Wendy Ashley, Paul Allan, Elaine Young, Anne M. |
author_sort | Yaghmoor, Rayan B. |
collection | PubMed |
description | Continuing cariogenic bacterial growth demineralizing dentine beneath a composite filling is the most common cause of tooth restoration failure. Novel composites with antibacterial polylysine (PLS) (0, 4, 6, or 8 wt%) in its filler phase were therefore produced. Remineralising monocalcium phosphate was also included at double the PLS weight. Antibacterial studies involved set composite disc placement in 1% sucrose-supplemented broth containing Streptococcus mutans (UA159). Relative surface bacterial biofilm mass (n = 4) after 24 h was determined by crystal violet-binding. Live/dead bacteria and biofilm thickness (n = 3) were assessed using confocal laser scanning microscopy (CLSM). To understand results and model possible in vivo benefits, cumulative PLS release from discs into water (n = 3) was determined by a ninhydrin assay. Results showed biofilm mass and thickness decreased linearly by 28% and 33%, respectively, upon increasing PLS from 0% to 8%. With 4, 6, and 8 wt% PLS, respectively, biofilm dead bacterial percentages and PLS release at 24 h were 20%, 60%, and 80% and 85, 163, and 241 μg/disc. Furthermore, initial PLS release was proportional to the square root of time and levelled after 1, 2, and 3 months at 13%, 28%, and 42%. This suggested diffusion controlled release from water-exposed composite surface layers of 65, 140, and 210 μm thickness, respectively. In conclusion, increasing PLS release initially in any gaps under the restoration to kill residual bacteria or longer-term following composite/tooth interface damage might help prevent recurrent caries. |
format | Online Article Text |
id | pubmed-7564959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75649592020-10-26 Effect of Novel Antibacterial Composites on Bacterial Biofilms Yaghmoor, Rayan B. Xia, Wendy Ashley, Paul Allan, Elaine Young, Anne M. J Funct Biomater Article Continuing cariogenic bacterial growth demineralizing dentine beneath a composite filling is the most common cause of tooth restoration failure. Novel composites with antibacterial polylysine (PLS) (0, 4, 6, or 8 wt%) in its filler phase were therefore produced. Remineralising monocalcium phosphate was also included at double the PLS weight. Antibacterial studies involved set composite disc placement in 1% sucrose-supplemented broth containing Streptococcus mutans (UA159). Relative surface bacterial biofilm mass (n = 4) after 24 h was determined by crystal violet-binding. Live/dead bacteria and biofilm thickness (n = 3) were assessed using confocal laser scanning microscopy (CLSM). To understand results and model possible in vivo benefits, cumulative PLS release from discs into water (n = 3) was determined by a ninhydrin assay. Results showed biofilm mass and thickness decreased linearly by 28% and 33%, respectively, upon increasing PLS from 0% to 8%. With 4, 6, and 8 wt% PLS, respectively, biofilm dead bacterial percentages and PLS release at 24 h were 20%, 60%, and 80% and 85, 163, and 241 μg/disc. Furthermore, initial PLS release was proportional to the square root of time and levelled after 1, 2, and 3 months at 13%, 28%, and 42%. This suggested diffusion controlled release from water-exposed composite surface layers of 65, 140, and 210 μm thickness, respectively. In conclusion, increasing PLS release initially in any gaps under the restoration to kill residual bacteria or longer-term following composite/tooth interface damage might help prevent recurrent caries. MDPI 2020-08-01 /pmc/articles/PMC7564959/ /pubmed/32752201 http://dx.doi.org/10.3390/jfb11030055 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yaghmoor, Rayan B. Xia, Wendy Ashley, Paul Allan, Elaine Young, Anne M. Effect of Novel Antibacterial Composites on Bacterial Biofilms |
title | Effect of Novel Antibacterial Composites on Bacterial Biofilms |
title_full | Effect of Novel Antibacterial Composites on Bacterial Biofilms |
title_fullStr | Effect of Novel Antibacterial Composites on Bacterial Biofilms |
title_full_unstemmed | Effect of Novel Antibacterial Composites on Bacterial Biofilms |
title_short | Effect of Novel Antibacterial Composites on Bacterial Biofilms |
title_sort | effect of novel antibacterial composites on bacterial biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564959/ https://www.ncbi.nlm.nih.gov/pubmed/32752201 http://dx.doi.org/10.3390/jfb11030055 |
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