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Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production

To differentiate foot-and-mouth disease (FMD)-infected animals from vaccinated livestock, non-structural proteins (NSPs) must be removed during the FMD vaccine manufacturing process. Currently, NSPs cannot be selectively removed from FMD virus (FMDV) culture supernatant. Therefore, polyethylene glyc...

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Autores principales: Park, Sun Young, Lee, Jung-Min, Kim, Ah-Young, Park, Sang Hyun, Lee, Sim-In, Kim, Hyejin, Kim, Jae-Seok, Park, Jong-Hyeon, Ko, Young-Joon, Park, Choi-Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565013/
https://www.ncbi.nlm.nih.gov/pubmed/32867254
http://dx.doi.org/10.3390/vaccines8030483
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author Park, Sun Young
Lee, Jung-Min
Kim, Ah-Young
Park, Sang Hyun
Lee, Sim-In
Kim, Hyejin
Kim, Jae-Seok
Park, Jong-Hyeon
Ko, Young-Joon
Park, Choi-Kyu
author_facet Park, Sun Young
Lee, Jung-Min
Kim, Ah-Young
Park, Sang Hyun
Lee, Sim-In
Kim, Hyejin
Kim, Jae-Seok
Park, Jong-Hyeon
Ko, Young-Joon
Park, Choi-Kyu
author_sort Park, Sun Young
collection PubMed
description To differentiate foot-and-mouth disease (FMD)-infected animals from vaccinated livestock, non-structural proteins (NSPs) must be removed during the FMD vaccine manufacturing process. Currently, NSPs cannot be selectively removed from FMD virus (FMDV) culture supernatant. Therefore, polyethylene glycol (PEG) is utilized to partially separate FMDV from NSPs. However, some NSPs remain in the FMD vaccine, which after repeated immunization, may elicit NSP antibodies in some livestock. To address this drawback, chloroform at a concentration of more than 2% (v/v) was found to remove NSP efficiently without damaging the FMDV particles. Contrary to the PEG-treated vaccine that showed positive NSP antibody responses after the third immunization in goats, the chloroform-treated vaccine did not induce NSP antibodies. In addition to this enhanced vaccine purity, this new method using chloroform could maximize antigen recovery and the vaccine production time could be shortened by two days due to omission of the PEG processing phase. To our knowledge, this is the first report to remove NSPs from FMDV culture supernatant by chemical addition. This novel method could revolutionize the conventional processes of FMD vaccine production.
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spelling pubmed-75650132020-10-26 Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production Park, Sun Young Lee, Jung-Min Kim, Ah-Young Park, Sang Hyun Lee, Sim-In Kim, Hyejin Kim, Jae-Seok Park, Jong-Hyeon Ko, Young-Joon Park, Choi-Kyu Vaccines (Basel) Article To differentiate foot-and-mouth disease (FMD)-infected animals from vaccinated livestock, non-structural proteins (NSPs) must be removed during the FMD vaccine manufacturing process. Currently, NSPs cannot be selectively removed from FMD virus (FMDV) culture supernatant. Therefore, polyethylene glycol (PEG) is utilized to partially separate FMDV from NSPs. However, some NSPs remain in the FMD vaccine, which after repeated immunization, may elicit NSP antibodies in some livestock. To address this drawback, chloroform at a concentration of more than 2% (v/v) was found to remove NSP efficiently without damaging the FMDV particles. Contrary to the PEG-treated vaccine that showed positive NSP antibody responses after the third immunization in goats, the chloroform-treated vaccine did not induce NSP antibodies. In addition to this enhanced vaccine purity, this new method using chloroform could maximize antigen recovery and the vaccine production time could be shortened by two days due to omission of the PEG processing phase. To our knowledge, this is the first report to remove NSPs from FMDV culture supernatant by chemical addition. This novel method could revolutionize the conventional processes of FMD vaccine production. MDPI 2020-08-27 /pmc/articles/PMC7565013/ /pubmed/32867254 http://dx.doi.org/10.3390/vaccines8030483 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Sun Young
Lee, Jung-Min
Kim, Ah-Young
Park, Sang Hyun
Lee, Sim-In
Kim, Hyejin
Kim, Jae-Seok
Park, Jong-Hyeon
Ko, Young-Joon
Park, Choi-Kyu
Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
title Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
title_full Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
title_fullStr Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
title_full_unstemmed Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
title_short Efficient Removal of Non-Structural Protein Using Chloroform for Foot-and-Mouth Disease Vaccine Production
title_sort efficient removal of non-structural protein using chloroform for foot-and-mouth disease vaccine production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565013/
https://www.ncbi.nlm.nih.gov/pubmed/32867254
http://dx.doi.org/10.3390/vaccines8030483
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