Cargando…

Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach

This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determi...

Descripción completa

Detalles Bibliográficos
Autores principales: Pichon, Maxime, Tran, Cong Tri, Motillon, Gaëtan, Debiais, Charlotte, Gautier, Sylvain, Aballea, Marie, Cremniter, Julie, Vasseur, Philippe, Tougeron, David, Garcia, Magali, Garnier, Martine, Bodet, Charles, Faure, Jean Pierre, Burucoa, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565078/
https://www.ncbi.nlm.nih.gov/pubmed/32878081
http://dx.doi.org/10.3390/jcm9092812
_version_ 1783595859208830976
author Pichon, Maxime
Tran, Cong Tri
Motillon, Gaëtan
Debiais, Charlotte
Gautier, Sylvain
Aballea, Marie
Cremniter, Julie
Vasseur, Philippe
Tougeron, David
Garcia, Magali
Garnier, Martine
Bodet, Charles
Faure, Jean Pierre
Burucoa, Christophe
author_facet Pichon, Maxime
Tran, Cong Tri
Motillon, Gaëtan
Debiais, Charlotte
Gautier, Sylvain
Aballea, Marie
Cremniter, Julie
Vasseur, Philippe
Tougeron, David
Garcia, Magali
Garnier, Martine
Bodet, Charles
Faure, Jean Pierre
Burucoa, Christophe
author_sort Pichon, Maxime
collection PubMed
description This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determined using culture and RT-PCR on multiple gastric sites (88, 176, and 101 biopsies per patient). Diversity of H. pylori strains was studied using antibiotic susceptibility testing, random amplified polymorphism DNA (RAPD) typing and cagA gene detection on single-colony isolates (44, 96, and 49 isolates per patient). H. pylori was detected in nearly all analyzed sites (354/365 biopsies, 97%). Antral density was higher in one patient only. The three stomachs were almost exclusively infected by an antibiotic-susceptible strain. One clarithromycin-resistant isolate in one biopsy was detected in two stomachs (1/44 and 1/49 isolates), while in the third one, eight (8/96 isolates) metronidazole-resistant isolates were detected. DNA typing showed infection with cagA-negative strains for one patient, cagA-positive strains for a second patient and the third patient was infected with two different strains of distinct cagA genotypes. Infection with H. pylori is shown to spread to the whole surface of the stomach, but a possibility of minor sub-population of antibiotic-resistant clones, undetectable in routine practice.
format Online
Article
Text
id pubmed-7565078
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75650782020-10-26 Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach Pichon, Maxime Tran, Cong Tri Motillon, Gaëtan Debiais, Charlotte Gautier, Sylvain Aballea, Marie Cremniter, Julie Vasseur, Philippe Tougeron, David Garcia, Magali Garnier, Martine Bodet, Charles Faure, Jean Pierre Burucoa, Christophe J Clin Med Article This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determined using culture and RT-PCR on multiple gastric sites (88, 176, and 101 biopsies per patient). Diversity of H. pylori strains was studied using antibiotic susceptibility testing, random amplified polymorphism DNA (RAPD) typing and cagA gene detection on single-colony isolates (44, 96, and 49 isolates per patient). H. pylori was detected in nearly all analyzed sites (354/365 biopsies, 97%). Antral density was higher in one patient only. The three stomachs were almost exclusively infected by an antibiotic-susceptible strain. One clarithromycin-resistant isolate in one biopsy was detected in two stomachs (1/44 and 1/49 isolates), while in the third one, eight (8/96 isolates) metronidazole-resistant isolates were detected. DNA typing showed infection with cagA-negative strains for one patient, cagA-positive strains for a second patient and the third patient was infected with two different strains of distinct cagA genotypes. Infection with H. pylori is shown to spread to the whole surface of the stomach, but a possibility of minor sub-population of antibiotic-resistant clones, undetectable in routine practice. MDPI 2020-08-31 /pmc/articles/PMC7565078/ /pubmed/32878081 http://dx.doi.org/10.3390/jcm9092812 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pichon, Maxime
Tran, Cong Tri
Motillon, Gaëtan
Debiais, Charlotte
Gautier, Sylvain
Aballea, Marie
Cremniter, Julie
Vasseur, Philippe
Tougeron, David
Garcia, Magali
Garnier, Martine
Bodet, Charles
Faure, Jean Pierre
Burucoa, Christophe
Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
title Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
title_full Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
title_fullStr Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
title_full_unstemmed Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
title_short Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
title_sort where to biopsy to detect helicobacter pylori and how many biopsies are needed to detect antibiotic resistance in a human stomach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565078/
https://www.ncbi.nlm.nih.gov/pubmed/32878081
http://dx.doi.org/10.3390/jcm9092812
work_keys_str_mv AT pichonmaxime wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT trancongtri wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT motillongaetan wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT debiaischarlotte wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT gautiersylvain wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT aballeamarie wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT cremniterjulie wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT vasseurphilippe wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT tougerondavid wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT garciamagali wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT garniermartine wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT bodetcharles wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT faurejeanpierre wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach
AT burucoachristophe wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach