Cargando…
Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach
This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determi...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565078/ https://www.ncbi.nlm.nih.gov/pubmed/32878081 http://dx.doi.org/10.3390/jcm9092812 |
_version_ | 1783595859208830976 |
---|---|
author | Pichon, Maxime Tran, Cong Tri Motillon, Gaëtan Debiais, Charlotte Gautier, Sylvain Aballea, Marie Cremniter, Julie Vasseur, Philippe Tougeron, David Garcia, Magali Garnier, Martine Bodet, Charles Faure, Jean Pierre Burucoa, Christophe |
author_facet | Pichon, Maxime Tran, Cong Tri Motillon, Gaëtan Debiais, Charlotte Gautier, Sylvain Aballea, Marie Cremniter, Julie Vasseur, Philippe Tougeron, David Garcia, Magali Garnier, Martine Bodet, Charles Faure, Jean Pierre Burucoa, Christophe |
author_sort | Pichon, Maxime |
collection | PubMed |
description | This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determined using culture and RT-PCR on multiple gastric sites (88, 176, and 101 biopsies per patient). Diversity of H. pylori strains was studied using antibiotic susceptibility testing, random amplified polymorphism DNA (RAPD) typing and cagA gene detection on single-colony isolates (44, 96, and 49 isolates per patient). H. pylori was detected in nearly all analyzed sites (354/365 biopsies, 97%). Antral density was higher in one patient only. The three stomachs were almost exclusively infected by an antibiotic-susceptible strain. One clarithromycin-resistant isolate in one biopsy was detected in two stomachs (1/44 and 1/49 isolates), while in the third one, eight (8/96 isolates) metronidazole-resistant isolates were detected. DNA typing showed infection with cagA-negative strains for one patient, cagA-positive strains for a second patient and the third patient was infected with two different strains of distinct cagA genotypes. Infection with H. pylori is shown to spread to the whole surface of the stomach, but a possibility of minor sub-population of antibiotic-resistant clones, undetectable in routine practice. |
format | Online Article Text |
id | pubmed-7565078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75650782020-10-26 Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach Pichon, Maxime Tran, Cong Tri Motillon, Gaëtan Debiais, Charlotte Gautier, Sylvain Aballea, Marie Cremniter, Julie Vasseur, Philippe Tougeron, David Garcia, Magali Garnier, Martine Bodet, Charles Faure, Jean Pierre Burucoa, Christophe J Clin Med Article This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determined using culture and RT-PCR on multiple gastric sites (88, 176, and 101 biopsies per patient). Diversity of H. pylori strains was studied using antibiotic susceptibility testing, random amplified polymorphism DNA (RAPD) typing and cagA gene detection on single-colony isolates (44, 96, and 49 isolates per patient). H. pylori was detected in nearly all analyzed sites (354/365 biopsies, 97%). Antral density was higher in one patient only. The three stomachs were almost exclusively infected by an antibiotic-susceptible strain. One clarithromycin-resistant isolate in one biopsy was detected in two stomachs (1/44 and 1/49 isolates), while in the third one, eight (8/96 isolates) metronidazole-resistant isolates were detected. DNA typing showed infection with cagA-negative strains for one patient, cagA-positive strains for a second patient and the third patient was infected with two different strains of distinct cagA genotypes. Infection with H. pylori is shown to spread to the whole surface of the stomach, but a possibility of minor sub-population of antibiotic-resistant clones, undetectable in routine practice. MDPI 2020-08-31 /pmc/articles/PMC7565078/ /pubmed/32878081 http://dx.doi.org/10.3390/jcm9092812 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pichon, Maxime Tran, Cong Tri Motillon, Gaëtan Debiais, Charlotte Gautier, Sylvain Aballea, Marie Cremniter, Julie Vasseur, Philippe Tougeron, David Garcia, Magali Garnier, Martine Bodet, Charles Faure, Jean Pierre Burucoa, Christophe Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach |
title | Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach |
title_full | Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach |
title_fullStr | Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach |
title_full_unstemmed | Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach |
title_short | Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach |
title_sort | where to biopsy to detect helicobacter pylori and how many biopsies are needed to detect antibiotic resistance in a human stomach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565078/ https://www.ncbi.nlm.nih.gov/pubmed/32878081 http://dx.doi.org/10.3390/jcm9092812 |
work_keys_str_mv | AT pichonmaxime wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT trancongtri wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT motillongaetan wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT debiaischarlotte wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT gautiersylvain wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT aballeamarie wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT cremniterjulie wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT vasseurphilippe wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT tougerondavid wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT garciamagali wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT garniermartine wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT bodetcharles wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT faurejeanpierre wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach AT burucoachristophe wheretobiopsytodetecthelicobacterpyloriandhowmanybiopsiesareneededtodetectantibioticresistanceinahumanstomach |