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In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice
Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-pro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565435/ https://www.ncbi.nlm.nih.gov/pubmed/32947881 http://dx.doi.org/10.3390/cells9092104 |
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author | Cuffaro, Bernardo Assohoun, Aka L. W. Boutillier, Denise Súkeníková, Lenka Desramaut, Jérémy Boudebbouze, Samira Salomé-Desnoulez, Sophie Hrdý, Jiří Waligora-Dupriet, Anne-Judith Maguin, Emmanuelle Grangette, Corinne |
author_facet | Cuffaro, Bernardo Assohoun, Aka L. W. Boutillier, Denise Súkeníková, Lenka Desramaut, Jérémy Boudebbouze, Samira Salomé-Desnoulez, Sophie Hrdý, Jiří Waligora-Dupriet, Anne-Judith Maguin, Emmanuelle Grangette, Corinne |
author_sort | Cuffaro, Bernardo |
collection | PubMed |
description | Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five Parabacteroides distasonis strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of P. distasonis DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4(+) T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD. |
format | Online Article Text |
id | pubmed-7565435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75654352020-10-26 In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice Cuffaro, Bernardo Assohoun, Aka L. W. Boutillier, Denise Súkeníková, Lenka Desramaut, Jérémy Boudebbouze, Samira Salomé-Desnoulez, Sophie Hrdý, Jiří Waligora-Dupriet, Anne-Judith Maguin, Emmanuelle Grangette, Corinne Cells Article Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five Parabacteroides distasonis strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of P. distasonis DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4(+) T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD. MDPI 2020-09-16 /pmc/articles/PMC7565435/ /pubmed/32947881 http://dx.doi.org/10.3390/cells9092104 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cuffaro, Bernardo Assohoun, Aka L. W. Boutillier, Denise Súkeníková, Lenka Desramaut, Jérémy Boudebbouze, Samira Salomé-Desnoulez, Sophie Hrdý, Jiří Waligora-Dupriet, Anne-Judith Maguin, Emmanuelle Grangette, Corinne In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice |
title | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice |
title_full | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice |
title_fullStr | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice |
title_full_unstemmed | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice |
title_short | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice |
title_sort | in vitro characterization of gut microbiota-derived commensal strains: selection of parabacteroides distasonis strains alleviating tnbs-induced colitis in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565435/ https://www.ncbi.nlm.nih.gov/pubmed/32947881 http://dx.doi.org/10.3390/cells9092104 |
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