Cargando…

Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells

Artemisia rupestris L. has long been used as a traditional herbal medicine owing to its immunomodulatory activity. Aqueous extracts of Artemisia rupestris L. (AEAR) contain the main functional component and can activate the maturation of dendritic cells (DCs) and enhance the adaptive immunity as the...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Yu, Wang, DanYang, Li, QuanXiao, He, Jiang, Wang, Bin, Li, Jinyao, Zhang, Ailian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565461/
https://www.ncbi.nlm.nih.gov/pubmed/32933167
http://dx.doi.org/10.3390/vaccines8030525
_version_ 1783595937836302336
author Yang, Yu
Wang, DanYang
Li, QuanXiao
He, Jiang
Wang, Bin
Li, Jinyao
Zhang, Ailian
author_facet Yang, Yu
Wang, DanYang
Li, QuanXiao
He, Jiang
Wang, Bin
Li, Jinyao
Zhang, Ailian
author_sort Yang, Yu
collection PubMed
description Artemisia rupestris L. has long been used as a traditional herbal medicine owing to its immunomodulatory activity. Aqueous extracts of Artemisia rupestris L. (AEAR) contain the main functional component and can activate the maturation of dendritic cells (DCs) and enhance the adaptive immunity as the adjuvant against infections. To explore the underlying mechanism of immunomodulatory activities of AEAR, DCs were produced from bone-marrow cells of mice and the effects of AEAR on cell viability were assessed by the Cell Counting Kit 8 (CCK8) method and annexin V/propidium iodide staining assays. Then, the effects of AEAR on the morphology, maturation, and function of DCs were detected using a microscope, flow cytometry-based surface receptor characterization, and endocytosis assays. The secretion levels of cytokines were then analyzed with enzyme-linked immunosorbent assay (ELISA). The activation state of DCs was evaluated by the mixed lymphocyte reaction (MLR). The activity of MAPKs and NF-κB pathways, which were involved in the regulation of AEAR on DCs, was further detected by Western blot. AEAR did not have a cytotoxic effect on DCs or mouse splenocytes. AEAR remarkably enhanced the phenotypic maturation of DCs and promoted the expression of costimulatory molecules and the secretion of cytokines in DCs. AEAR also significantly decreased the phagocytic ability of DCs and augmented the abilities of DCs to present antigens and stimulate allogeneic T-cell proliferation. Simultaneously, AEAR potently activated toll-like receptor (TLR)4-/TLR2-related MAPKs and induced the degradation of IκB and the translocation of NF-κB. In short, AEAR can profoundly enhance the immune-modulating activities of DCs via TLR4-/TLR2-mediated activation of MAPKs and NF-κB signaling pathways and is a promising candidate immunopotentiator for vaccines.
format Online
Article
Text
id pubmed-7565461
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75654612020-10-26 Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells Yang, Yu Wang, DanYang Li, QuanXiao He, Jiang Wang, Bin Li, Jinyao Zhang, Ailian Vaccines (Basel) Article Artemisia rupestris L. has long been used as a traditional herbal medicine owing to its immunomodulatory activity. Aqueous extracts of Artemisia rupestris L. (AEAR) contain the main functional component and can activate the maturation of dendritic cells (DCs) and enhance the adaptive immunity as the adjuvant against infections. To explore the underlying mechanism of immunomodulatory activities of AEAR, DCs were produced from bone-marrow cells of mice and the effects of AEAR on cell viability were assessed by the Cell Counting Kit 8 (CCK8) method and annexin V/propidium iodide staining assays. Then, the effects of AEAR on the morphology, maturation, and function of DCs were detected using a microscope, flow cytometry-based surface receptor characterization, and endocytosis assays. The secretion levels of cytokines were then analyzed with enzyme-linked immunosorbent assay (ELISA). The activation state of DCs was evaluated by the mixed lymphocyte reaction (MLR). The activity of MAPKs and NF-κB pathways, which were involved in the regulation of AEAR on DCs, was further detected by Western blot. AEAR did not have a cytotoxic effect on DCs or mouse splenocytes. AEAR remarkably enhanced the phenotypic maturation of DCs and promoted the expression of costimulatory molecules and the secretion of cytokines in DCs. AEAR also significantly decreased the phagocytic ability of DCs and augmented the abilities of DCs to present antigens and stimulate allogeneic T-cell proliferation. Simultaneously, AEAR potently activated toll-like receptor (TLR)4-/TLR2-related MAPKs and induced the degradation of IκB and the translocation of NF-κB. In short, AEAR can profoundly enhance the immune-modulating activities of DCs via TLR4-/TLR2-mediated activation of MAPKs and NF-κB signaling pathways and is a promising candidate immunopotentiator for vaccines. MDPI 2020-09-13 /pmc/articles/PMC7565461/ /pubmed/32933167 http://dx.doi.org/10.3390/vaccines8030525 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Yu
Wang, DanYang
Li, QuanXiao
He, Jiang
Wang, Bin
Li, Jinyao
Zhang, Ailian
Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
title Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
title_full Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
title_fullStr Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
title_full_unstemmed Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
title_short Immune-Enhancing Activity of Aqueous Extracts from Artemisia rupestris L. via MAPK and NF-kB Pathways of TLR4/TLR2 Downstream in Dendritic Cells
title_sort immune-enhancing activity of aqueous extracts from artemisia rupestris l. via mapk and nf-kb pathways of tlr4/tlr2 downstream in dendritic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565461/
https://www.ncbi.nlm.nih.gov/pubmed/32933167
http://dx.doi.org/10.3390/vaccines8030525
work_keys_str_mv AT yangyu immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells
AT wangdanyang immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells
AT liquanxiao immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells
AT hejiang immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells
AT wangbin immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells
AT lijinyao immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells
AT zhangailian immuneenhancingactivityofaqueousextractsfromartemisiarupestrislviamapkandnfkbpathwaysoftlr4tlr2downstreamindendriticcells