Cargando…

Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid

Encapsulin is a class of nanocompartments that is unique in bacteria and archaea to confine enzymatic activities and sequester toxic reaction products. Here we present a 2.87 Å resolution cryo-EM structure of Thermotoga maritima encapsulin with heterologous protein complex loaded. It is the first su...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiong, Xiansong, Sun, Chen, Vago, Frank S., Klose, Thomas, Zhu, Jiankang, Jiang, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565527/
https://www.ncbi.nlm.nih.gov/pubmed/32961724
http://dx.doi.org/10.3390/biom10091342
_version_ 1783595953074208768
author Xiong, Xiansong
Sun, Chen
Vago, Frank S.
Klose, Thomas
Zhu, Jiankang
Jiang, Wen
author_facet Xiong, Xiansong
Sun, Chen
Vago, Frank S.
Klose, Thomas
Zhu, Jiankang
Jiang, Wen
author_sort Xiong, Xiansong
collection PubMed
description Encapsulin is a class of nanocompartments that is unique in bacteria and archaea to confine enzymatic activities and sequester toxic reaction products. Here we present a 2.87 Å resolution cryo-EM structure of Thermotoga maritima encapsulin with heterologous protein complex loaded. It is the first successful case of expressing encapsulin and heterologous cargo protein in the insect cell system. Although we failed to reconstruct the cargo protein complex structure due to the signal interference of the capsid shell, we were able to observe some unique features of the cargo-loaded encapsulin shell, for example, an extra density at the fivefold pore that has not been reported before. These results would lead to a more complete understanding of the encapsulin cargo assembly process of T. maritima.
format Online
Article
Text
id pubmed-7565527
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75655272020-10-26 Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid Xiong, Xiansong Sun, Chen Vago, Frank S. Klose, Thomas Zhu, Jiankang Jiang, Wen Biomolecules Article Encapsulin is a class of nanocompartments that is unique in bacteria and archaea to confine enzymatic activities and sequester toxic reaction products. Here we present a 2.87 Å resolution cryo-EM structure of Thermotoga maritima encapsulin with heterologous protein complex loaded. It is the first successful case of expressing encapsulin and heterologous cargo protein in the insect cell system. Although we failed to reconstruct the cargo protein complex structure due to the signal interference of the capsid shell, we were able to observe some unique features of the cargo-loaded encapsulin shell, for example, an extra density at the fivefold pore that has not been reported before. These results would lead to a more complete understanding of the encapsulin cargo assembly process of T. maritima. MDPI 2020-09-19 /pmc/articles/PMC7565527/ /pubmed/32961724 http://dx.doi.org/10.3390/biom10091342 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiong, Xiansong
Sun, Chen
Vago, Frank S.
Klose, Thomas
Zhu, Jiankang
Jiang, Wen
Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid
title Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid
title_full Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid
title_fullStr Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid
title_full_unstemmed Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid
title_short Cryo-EM Structure of Heterologous Protein Complex Loaded Thermotoga Maritima Encapsulin Capsid
title_sort cryo-em structure of heterologous protein complex loaded thermotoga maritima encapsulin capsid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565527/
https://www.ncbi.nlm.nih.gov/pubmed/32961724
http://dx.doi.org/10.3390/biom10091342
work_keys_str_mv AT xiongxiansong cryoemstructureofheterologousproteincomplexloadedthermotogamaritimaencapsulincapsid
AT sunchen cryoemstructureofheterologousproteincomplexloadedthermotogamaritimaencapsulincapsid
AT vagofranks cryoemstructureofheterologousproteincomplexloadedthermotogamaritimaencapsulincapsid
AT klosethomas cryoemstructureofheterologousproteincomplexloadedthermotogamaritimaencapsulincapsid
AT zhujiankang cryoemstructureofheterologousproteincomplexloadedthermotogamaritimaencapsulincapsid
AT jiangwen cryoemstructureofheterologousproteincomplexloadedthermotogamaritimaencapsulincapsid