Cargando…
Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report
Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes and several subtypes. HEV genotypes 3 and 4 (HEV3 and HEV4) are zoonotic. In Italy, the most part of HEV infections (80%) is due to autochthonous HEV3 circ...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565956/ https://www.ncbi.nlm.nih.gov/pubmed/32932776 http://dx.doi.org/10.3390/microorganisms8091393 |
_version_ | 1783596047065415680 |
---|---|
author | Aprea, Giuseppe Scattolini, Silvia D’Angelantonio, Daniela Chiaverini, Alexandra Di Lollo, Valeria Olivieri, Sabrina Marcacci, Maurilia Mangone, Iolanda Salucci, Stefania Antoci, Salvatore Cammà, Cesare Di Pasquale, Adriano Migliorati, Giacomo Pomilio, Francesco |
author_facet | Aprea, Giuseppe Scattolini, Silvia D’Angelantonio, Daniela Chiaverini, Alexandra Di Lollo, Valeria Olivieri, Sabrina Marcacci, Maurilia Mangone, Iolanda Salucci, Stefania Antoci, Salvatore Cammà, Cesare Di Pasquale, Adriano Migliorati, Giacomo Pomilio, Francesco |
author_sort | Aprea, Giuseppe |
collection | PubMed |
description | Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes and several subtypes. HEV genotypes 3 and 4 (HEV3 and HEV4) are zoonotic. In Italy, the most part of HEV infections (80%) is due to autochthonous HEV3 circulation of the virus, and the key role played by wild animals is generally accepted. Abruzzo is an Italian region officially considered an HEV “hot spot”, with subtype HEV3-c being up to now the only one reported among wild boars. During the year 2018–2019, a group of wild boars in Abruzzo were screened for HEV; positive RNA liver samples were subjected to HEV characterization by using the whole genome sequencing (WGS) approach methodology. This represents the first report about the detection of HEV-3 subtypes e and f in the wild boar population in this area. Since in Italy human infections from HEV 3-e and f have been associated with pork meat consumption, our findings deserve more in-depth analysis with the aim of evaluating any potential correlation between wild animals, the pork chain production and HEV human infections. |
format | Online Article Text |
id | pubmed-7565956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75659562020-10-26 Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report Aprea, Giuseppe Scattolini, Silvia D’Angelantonio, Daniela Chiaverini, Alexandra Di Lollo, Valeria Olivieri, Sabrina Marcacci, Maurilia Mangone, Iolanda Salucci, Stefania Antoci, Salvatore Cammà, Cesare Di Pasquale, Adriano Migliorati, Giacomo Pomilio, Francesco Microorganisms Article Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes and several subtypes. HEV genotypes 3 and 4 (HEV3 and HEV4) are zoonotic. In Italy, the most part of HEV infections (80%) is due to autochthonous HEV3 circulation of the virus, and the key role played by wild animals is generally accepted. Abruzzo is an Italian region officially considered an HEV “hot spot”, with subtype HEV3-c being up to now the only one reported among wild boars. During the year 2018–2019, a group of wild boars in Abruzzo were screened for HEV; positive RNA liver samples were subjected to HEV characterization by using the whole genome sequencing (WGS) approach methodology. This represents the first report about the detection of HEV-3 subtypes e and f in the wild boar population in this area. Since in Italy human infections from HEV 3-e and f have been associated with pork meat consumption, our findings deserve more in-depth analysis with the aim of evaluating any potential correlation between wild animals, the pork chain production and HEV human infections. MDPI 2020-09-11 /pmc/articles/PMC7565956/ /pubmed/32932776 http://dx.doi.org/10.3390/microorganisms8091393 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aprea, Giuseppe Scattolini, Silvia D’Angelantonio, Daniela Chiaverini, Alexandra Di Lollo, Valeria Olivieri, Sabrina Marcacci, Maurilia Mangone, Iolanda Salucci, Stefania Antoci, Salvatore Cammà, Cesare Di Pasquale, Adriano Migliorati, Giacomo Pomilio, Francesco Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report |
title | Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report |
title_full | Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report |
title_fullStr | Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report |
title_full_unstemmed | Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report |
title_short | Whole Genome Sequencing Characterization of HEV3-e and HEV3-f Subtypes among the Wild Boar Population in the Abruzzo Region, Italy: First Report |
title_sort | whole genome sequencing characterization of hev3-e and hev3-f subtypes among the wild boar population in the abruzzo region, italy: first report |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565956/ https://www.ncbi.nlm.nih.gov/pubmed/32932776 http://dx.doi.org/10.3390/microorganisms8091393 |
work_keys_str_mv | AT apreagiuseppe wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT scattolinisilvia wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT dangelantoniodaniela wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT chiaverinialexandra wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT dilollovaleria wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT olivierisabrina wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT marcaccimaurilia wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT mangoneiolanda wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT saluccistefania wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT antocisalvatore wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT cammacesare wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT dipasqualeadriano wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT miglioratigiacomo wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport AT pomiliofrancesco wholegenomesequencingcharacterizationofhev3eandhev3fsubtypesamongthewildboarpopulationintheabruzzoregionitalyfirstreport |