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Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
BACKGROUND AND AIM: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas m...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566264/ https://www.ncbi.nlm.nih.gov/pubmed/33132616 http://dx.doi.org/10.14202/vetworld.2020.1992-1997 |
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author | Ngamkala, Suchanit Angkawanish, Taweepoke Nokkaew, Weerapun Thongtip, Nikorn |
author_facet | Ngamkala, Suchanit Angkawanish, Taweepoke Nokkaew, Weerapun Thongtip, Nikorn |
author_sort | Ngamkala, Suchanit |
collection | PubMed |
description | BACKGROUND AND AIM: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas maximus) has been limited. The present serological investigation aimed to investigate the antibody response to Brucella abortus in captive Asian elephants in North Thailand. Moreover, further serological survey was also conducted to detect the antibody response to Brucella canis in stray dogs cohabiting the same area as the elephant herd. MATERIALS AND METHODS: Serum samples were collected from 40 captive Asian elephants and submitted for serological analysis based on B. abortus antigen using Rose Bengal plate test (RBPT) in combination with ethylenediaminetetraacetic acid-tube agglutination test (EDTA-TAT) as a supplementary test and by commercial indirect enzyme-linked immunosorbent assay (iELISA). In addition, serum samples were also obtained from 16 stray dogs that live nearby the elephant-raising area and were tested using commercial Dot-ELISA based on B. canis antigen. RESULTS: Serological analysis in captive Asian elephants showed 100% seronegative (40/40) from all serological tests response to B. abortus. For stray dogs, 12.5% (2/16) had a low positive reaction response to B. canis. CONCLUSION: The serological survey for brucellosis in Asian elephant was adapted and applied using RBPT, EDTA-TAT, and iELISA in the present study. For future evaluation, we recommended the use of a combination of serological tests with validation together with comparing by direct detection such as bacterial isolation to provide an appropriate brucellosis surveillance program in Asian elephants. In addition, the surveillance of stray dogs or multispecies habitation should be kept into considerations. |
format | Online Article Text |
id | pubmed-7566264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-75662642020-10-30 Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand Ngamkala, Suchanit Angkawanish, Taweepoke Nokkaew, Weerapun Thongtip, Nikorn Vet World Research Article BACKGROUND AND AIM: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas maximus) has been limited. The present serological investigation aimed to investigate the antibody response to Brucella abortus in captive Asian elephants in North Thailand. Moreover, further serological survey was also conducted to detect the antibody response to Brucella canis in stray dogs cohabiting the same area as the elephant herd. MATERIALS AND METHODS: Serum samples were collected from 40 captive Asian elephants and submitted for serological analysis based on B. abortus antigen using Rose Bengal plate test (RBPT) in combination with ethylenediaminetetraacetic acid-tube agglutination test (EDTA-TAT) as a supplementary test and by commercial indirect enzyme-linked immunosorbent assay (iELISA). In addition, serum samples were also obtained from 16 stray dogs that live nearby the elephant-raising area and were tested using commercial Dot-ELISA based on B. canis antigen. RESULTS: Serological analysis in captive Asian elephants showed 100% seronegative (40/40) from all serological tests response to B. abortus. For stray dogs, 12.5% (2/16) had a low positive reaction response to B. canis. CONCLUSION: The serological survey for brucellosis in Asian elephant was adapted and applied using RBPT, EDTA-TAT, and iELISA in the present study. For future evaluation, we recommended the use of a combination of serological tests with validation together with comparing by direct detection such as bacterial isolation to provide an appropriate brucellosis surveillance program in Asian elephants. In addition, the surveillance of stray dogs or multispecies habitation should be kept into considerations. Veterinary World 2020-09 2020-09-26 /pmc/articles/PMC7566264/ /pubmed/33132616 http://dx.doi.org/10.14202/vetworld.2020.1992-1997 Text en Copyright: © Ngamkala, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Ngamkala, Suchanit Angkawanish, Taweepoke Nokkaew, Weerapun Thongtip, Nikorn Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand |
title | Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand |
title_full | Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand |
title_fullStr | Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand |
title_full_unstemmed | Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand |
title_short | Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand |
title_sort | serological study on brucellosis in captive elephants (elephas maximus) and stray dogs in north thailand |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566264/ https://www.ncbi.nlm.nih.gov/pubmed/33132616 http://dx.doi.org/10.14202/vetworld.2020.1992-1997 |
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