Cargando…

Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand

BACKGROUND AND AIM: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas m...

Descripción completa

Detalles Bibliográficos
Autores principales: Ngamkala, Suchanit, Angkawanish, Taweepoke, Nokkaew, Weerapun, Thongtip, Nikorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566264/
https://www.ncbi.nlm.nih.gov/pubmed/33132616
http://dx.doi.org/10.14202/vetworld.2020.1992-1997
_version_ 1783596110398357504
author Ngamkala, Suchanit
Angkawanish, Taweepoke
Nokkaew, Weerapun
Thongtip, Nikorn
author_facet Ngamkala, Suchanit
Angkawanish, Taweepoke
Nokkaew, Weerapun
Thongtip, Nikorn
author_sort Ngamkala, Suchanit
collection PubMed
description BACKGROUND AND AIM: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas maximus) has been limited. The present serological investigation aimed to investigate the antibody response to Brucella abortus in captive Asian elephants in North Thailand. Moreover, further serological survey was also conducted to detect the antibody response to Brucella canis in stray dogs cohabiting the same area as the elephant herd. MATERIALS AND METHODS: Serum samples were collected from 40 captive Asian elephants and submitted for serological analysis based on B. abortus antigen using Rose Bengal plate test (RBPT) in combination with ethylenediaminetetraacetic acid-tube agglutination test (EDTA-TAT) as a supplementary test and by commercial indirect enzyme-linked immunosorbent assay (iELISA). In addition, serum samples were also obtained from 16 stray dogs that live nearby the elephant-raising area and were tested using commercial Dot-ELISA based on B. canis antigen. RESULTS: Serological analysis in captive Asian elephants showed 100% seronegative (40/40) from all serological tests response to B. abortus. For stray dogs, 12.5% (2/16) had a low positive reaction response to B. canis. CONCLUSION: The serological survey for brucellosis in Asian elephant was adapted and applied using RBPT, EDTA-TAT, and iELISA in the present study. For future evaluation, we recommended the use of a combination of serological tests with validation together with comparing by direct detection such as bacterial isolation to provide an appropriate brucellosis surveillance program in Asian elephants. In addition, the surveillance of stray dogs or multispecies habitation should be kept into considerations.
format Online
Article
Text
id pubmed-7566264
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-75662642020-10-30 Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand Ngamkala, Suchanit Angkawanish, Taweepoke Nokkaew, Weerapun Thongtip, Nikorn Vet World Research Article BACKGROUND AND AIM: Brucellosis is considered as an important zoonotic disease caused by various strains of Brucella in numerous host species. Although brucellosis has been reported in almost animal species, the relevance of brucellosis infection and diagnostic technique in Asian elephant (Elephas maximus) has been limited. The present serological investigation aimed to investigate the antibody response to Brucella abortus in captive Asian elephants in North Thailand. Moreover, further serological survey was also conducted to detect the antibody response to Brucella canis in stray dogs cohabiting the same area as the elephant herd. MATERIALS AND METHODS: Serum samples were collected from 40 captive Asian elephants and submitted for serological analysis based on B. abortus antigen using Rose Bengal plate test (RBPT) in combination with ethylenediaminetetraacetic acid-tube agglutination test (EDTA-TAT) as a supplementary test and by commercial indirect enzyme-linked immunosorbent assay (iELISA). In addition, serum samples were also obtained from 16 stray dogs that live nearby the elephant-raising area and were tested using commercial Dot-ELISA based on B. canis antigen. RESULTS: Serological analysis in captive Asian elephants showed 100% seronegative (40/40) from all serological tests response to B. abortus. For stray dogs, 12.5% (2/16) had a low positive reaction response to B. canis. CONCLUSION: The serological survey for brucellosis in Asian elephant was adapted and applied using RBPT, EDTA-TAT, and iELISA in the present study. For future evaluation, we recommended the use of a combination of serological tests with validation together with comparing by direct detection such as bacterial isolation to provide an appropriate brucellosis surveillance program in Asian elephants. In addition, the surveillance of stray dogs or multispecies habitation should be kept into considerations. Veterinary World 2020-09 2020-09-26 /pmc/articles/PMC7566264/ /pubmed/33132616 http://dx.doi.org/10.14202/vetworld.2020.1992-1997 Text en Copyright: © Ngamkala, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ngamkala, Suchanit
Angkawanish, Taweepoke
Nokkaew, Weerapun
Thongtip, Nikorn
Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
title Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
title_full Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
title_fullStr Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
title_full_unstemmed Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
title_short Serological study on brucellosis in captive elephants (Elephas maximus) and stray dogs in North Thailand
title_sort serological study on brucellosis in captive elephants (elephas maximus) and stray dogs in north thailand
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566264/
https://www.ncbi.nlm.nih.gov/pubmed/33132616
http://dx.doi.org/10.14202/vetworld.2020.1992-1997
work_keys_str_mv AT ngamkalasuchanit serologicalstudyonbrucellosisincaptiveelephantselephasmaximusandstraydogsinnorththailand
AT angkawanishtaweepoke serologicalstudyonbrucellosisincaptiveelephantselephasmaximusandstraydogsinnorththailand
AT nokkaewweerapun serologicalstudyonbrucellosisincaptiveelephantselephasmaximusandstraydogsinnorththailand
AT thongtipnikorn serologicalstudyonbrucellosisincaptiveelephantselephasmaximusandstraydogsinnorththailand