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Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia

BACKGROUND AND AIM: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia. MATERIALS AND METHODS: This study utilized 10 LAB...

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Autores principales: Harnentis, Harnentis, Marlida, Yetti, Nur, Yuliaty Shafan, Wizna, Wizna, Santi, Melia Afnida, Septiani, Nadia, Adzitey, Frederick, Huda, Nurul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566266/
https://www.ncbi.nlm.nih.gov/pubmed/33132606
http://dx.doi.org/10.14202/vetworld.2020.1922-1927
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author Harnentis, Harnentis
Marlida, Yetti
Nur, Yuliaty Shafan
Wizna, Wizna
Santi, Melia Afnida
Septiani, Nadia
Adzitey, Frederick
Huda, Nurul
author_facet Harnentis, Harnentis
Marlida, Yetti
Nur, Yuliaty Shafan
Wizna, Wizna
Santi, Melia Afnida
Septiani, Nadia
Adzitey, Frederick
Huda, Nurul
author_sort Harnentis, Harnentis
collection PubMed
description BACKGROUND AND AIM: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia. MATERIALS AND METHODS: This study utilized 10 LAB previously isolated from fermented buffalo milk (dadih), fermented fish (budu), and fermented cassava (tape) which have the ability to produce gamma-aminobutyric acid. The study commenced with the screening of LAB for certain properties, such as resistance to acid and bile salts, adhesion to mucosal surface, and antagonism against enteric pathogens (Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus). The promising isolates were identified through biochemical and gram staining methods. RESULTS: All isolates in this study were potential novel probiotics. They survived at a pH level of 2.5 for 3 h (55.27-98.18%) and 6 h (50.98-84.91%). Survival in bile at a concentration of 0.3% was 39.90-58.61% and the survival rate was 28.38-52.11% at a concentration of 0.5%. The inhibitory diameter ranged from 8.75 to 11.54 mm for E. coli, 7.02 to 13.42 mm for S. aureus, and 12.49 to 19.00 mm for S. Enteritidis. All the isolates (84.5-92%) exhibited the ability to adhere to mucosal surfaces. This study revealed that all the isolates were potential probiotics but N16 proved to be superior because it was viable at a pH level of 2 (84.91%) and it had a good survival rate in bile salts assay (55.07%). This isolate was identified as Lactobacillus spp., Gram-positive bacilli bacteria, and tested negative in both the catalase and oxidase tests. CONCLUSION: All the isolates in this study may be used as probiotics, with isolate N16 (Lactobacillus spp.) as the most promising novel probiotic for poultry applications based on its ability to inhibit pathogenic bacteria.
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spelling pubmed-75662662020-10-30 Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia Harnentis, Harnentis Marlida, Yetti Nur, Yuliaty Shafan Wizna, Wizna Santi, Melia Afnida Septiani, Nadia Adzitey, Frederick Huda, Nurul Vet World Research Article BACKGROUND AND AIM: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia. MATERIALS AND METHODS: This study utilized 10 LAB previously isolated from fermented buffalo milk (dadih), fermented fish (budu), and fermented cassava (tape) which have the ability to produce gamma-aminobutyric acid. The study commenced with the screening of LAB for certain properties, such as resistance to acid and bile salts, adhesion to mucosal surface, and antagonism against enteric pathogens (Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus). The promising isolates were identified through biochemical and gram staining methods. RESULTS: All isolates in this study were potential novel probiotics. They survived at a pH level of 2.5 for 3 h (55.27-98.18%) and 6 h (50.98-84.91%). Survival in bile at a concentration of 0.3% was 39.90-58.61% and the survival rate was 28.38-52.11% at a concentration of 0.5%. The inhibitory diameter ranged from 8.75 to 11.54 mm for E. coli, 7.02 to 13.42 mm for S. aureus, and 12.49 to 19.00 mm for S. Enteritidis. All the isolates (84.5-92%) exhibited the ability to adhere to mucosal surfaces. This study revealed that all the isolates were potential probiotics but N16 proved to be superior because it was viable at a pH level of 2 (84.91%) and it had a good survival rate in bile salts assay (55.07%). This isolate was identified as Lactobacillus spp., Gram-positive bacilli bacteria, and tested negative in both the catalase and oxidase tests. CONCLUSION: All the isolates in this study may be used as probiotics, with isolate N16 (Lactobacillus spp.) as the most promising novel probiotic for poultry applications based on its ability to inhibit pathogenic bacteria. Veterinary World 2020-09 2020-09-19 /pmc/articles/PMC7566266/ /pubmed/33132606 http://dx.doi.org/10.14202/vetworld.2020.1922-1927 Text en Copyright: © Harnentis, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Harnentis, Harnentis
Marlida, Yetti
Nur, Yuliaty Shafan
Wizna, Wizna
Santi, Melia Afnida
Septiani, Nadia
Adzitey, Frederick
Huda, Nurul
Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
title Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
title_full Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
title_fullStr Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
title_full_unstemmed Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
title_short Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
title_sort novel probiotic lactic acid bacteria isolated from indigenous fermented foods from west sumatera, indonesia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566266/
https://www.ncbi.nlm.nih.gov/pubmed/33132606
http://dx.doi.org/10.14202/vetworld.2020.1922-1927
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