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Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential

Phytophagous insect larvae feed on plants containing secondary metabolic products with biological activity against other predatory organisms. Phytophagous insects can use their specialised metabolic systems to covert these secondary metabolic products into compounds with therapeutic properties usefu...

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Autores principales: Nakane, Wakana, Nakamura, Hisashi, Nakazato, Takeru, Kaminaga, Natsuki, Nakano, Miho, Sakamoto, Takuma, Nishiko, Maaya, Bono, Hidemasa, Ogiwara, Isao, Kitano, Yoshikazu, Iwabuchi, Kikuo, Kinoshita, Kaoru, Simpson, Richard J., Tabunoki, Hiroko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566601/
https://www.ncbi.nlm.nih.gov/pubmed/33060804
http://dx.doi.org/10.1038/s41598-020-74590-z
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author Nakane, Wakana
Nakamura, Hisashi
Nakazato, Takeru
Kaminaga, Natsuki
Nakano, Miho
Sakamoto, Takuma
Nishiko, Maaya
Bono, Hidemasa
Ogiwara, Isao
Kitano, Yoshikazu
Iwabuchi, Kikuo
Kinoshita, Kaoru
Simpson, Richard J.
Tabunoki, Hiroko
author_facet Nakane, Wakana
Nakamura, Hisashi
Nakazato, Takeru
Kaminaga, Natsuki
Nakano, Miho
Sakamoto, Takuma
Nishiko, Maaya
Bono, Hidemasa
Ogiwara, Isao
Kitano, Yoshikazu
Iwabuchi, Kikuo
Kinoshita, Kaoru
Simpson, Richard J.
Tabunoki, Hiroko
author_sort Nakane, Wakana
collection PubMed
description Phytophagous insect larvae feed on plants containing secondary metabolic products with biological activity against other predatory organisms. Phytophagous insects can use their specialised metabolic systems to covert these secondary metabolic products into compounds with therapeutic properties useful to mankind. Some Asians drink tea decoctions made from phytophagous insect frass which is believed to be effective against inflammatory diseases. However, insects that can convert plant-derived secondary metabolic products into useful human therapeutic agents remain poorly studied. Here, we constructed the TUATinsecta database by integrating publicly plant/insect datasets for the purpose of selecting insect species. Using TUAT-insecta we selected the Asian swallowtail butterfly, Papilio xuthus larvae fed on several species of Rutaceous plants and examined whether the plant-derived secondary metabolites, especially those present in frass, were chemically altered or not. We extracted metabolic products from frass using three organic solvents with different polarities, and evaluated solvent fractions for their cytotoxic effects against several human cell lines. We found that chloroform frass extracts from P. xuthus larvae fed on Poncirus trifoliata leaves contained significant cytotoxic activity. Our findings demonstrate that screening of insect species using the ‘TUATinsecta’ database provides an important pipeline for discovering novel therapeutic agents that might be useful for mankind.
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spelling pubmed-75666012020-10-19 Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential Nakane, Wakana Nakamura, Hisashi Nakazato, Takeru Kaminaga, Natsuki Nakano, Miho Sakamoto, Takuma Nishiko, Maaya Bono, Hidemasa Ogiwara, Isao Kitano, Yoshikazu Iwabuchi, Kikuo Kinoshita, Kaoru Simpson, Richard J. Tabunoki, Hiroko Sci Rep Article Phytophagous insect larvae feed on plants containing secondary metabolic products with biological activity against other predatory organisms. Phytophagous insects can use their specialised metabolic systems to covert these secondary metabolic products into compounds with therapeutic properties useful to mankind. Some Asians drink tea decoctions made from phytophagous insect frass which is believed to be effective against inflammatory diseases. However, insects that can convert plant-derived secondary metabolic products into useful human therapeutic agents remain poorly studied. Here, we constructed the TUATinsecta database by integrating publicly plant/insect datasets for the purpose of selecting insect species. Using TUAT-insecta we selected the Asian swallowtail butterfly, Papilio xuthus larvae fed on several species of Rutaceous plants and examined whether the plant-derived secondary metabolites, especially those present in frass, were chemically altered or not. We extracted metabolic products from frass using three organic solvents with different polarities, and evaluated solvent fractions for their cytotoxic effects against several human cell lines. We found that chloroform frass extracts from P. xuthus larvae fed on Poncirus trifoliata leaves contained significant cytotoxic activity. Our findings demonstrate that screening of insect species using the ‘TUATinsecta’ database provides an important pipeline for discovering novel therapeutic agents that might be useful for mankind. Nature Publishing Group UK 2020-10-15 /pmc/articles/PMC7566601/ /pubmed/33060804 http://dx.doi.org/10.1038/s41598-020-74590-z Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nakane, Wakana
Nakamura, Hisashi
Nakazato, Takeru
Kaminaga, Natsuki
Nakano, Miho
Sakamoto, Takuma
Nishiko, Maaya
Bono, Hidemasa
Ogiwara, Isao
Kitano, Yoshikazu
Iwabuchi, Kikuo
Kinoshita, Kaoru
Simpson, Richard J.
Tabunoki, Hiroko
Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
title Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
title_full Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
title_fullStr Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
title_full_unstemmed Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
title_short Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
title_sort construction of tuatinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566601/
https://www.ncbi.nlm.nih.gov/pubmed/33060804
http://dx.doi.org/10.1038/s41598-020-74590-z
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