An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9

Protoplasts are commonly used in genetic and breeding research. In this study, the isolation of sorghum protoplasts was optimized and applied to transient gene expression and editing by CRISPR/Cas9. The protoplast was most viable in 0.5 M mannitol, which was the highest of three concentrations after...

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Detalles Bibliográficos
Autores principales: Meng, Ruirui, Wang, Chenchen, Wang, Lihua, Liu, Yanlong, Zhan, Qiuwen, Zheng, Jiacheng, Li, Jieqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566750/
https://www.ncbi.nlm.nih.gov/pubmed/33083135
http://dx.doi.org/10.7717/peerj.10077

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566750/
https://www.ncbi.nlm.nih.gov/pubmed/33083135
http://dx.doi.org/10.7717/peerj.10077

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