Cargando…
An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9
Protoplasts are commonly used in genetic and breeding research. In this study, the isolation of sorghum protoplasts was optimized and applied to transient gene expression and editing by CRISPR/Cas9. The protoplast was most viable in 0.5 M mannitol, which was the highest of three concentrations after...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
PeerJ Inc.
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566750/ https://www.ncbi.nlm.nih.gov/pubmed/33083135 http://dx.doi.org/10.7717/peerj.10077 |