A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates

We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted lucifer...

Descripción completa

Detalles Bibliográficos
Autores principales: Tossberg, John T, Esmond, Tashawna M, Aune, Thomas M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566775/
https://www.ncbi.nlm.nih.gov/pubmed/32815735
http://dx.doi.org/10.2144/btn-2020-0037
_version_ 1783596194242494464
author Tossberg, John T
Esmond, Tashawna M
Aune, Thomas M
author_facet Tossberg, John T
Esmond, Tashawna M
Aune, Thomas M
author_sort Tossberg, John T
collection PubMed
description We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted luciferase, enhanced green fluorescence protein, IL-4, and IL-12A and IL-12B to form active IL-12. We propose that this method offers a cost- and time-saving alternative to plasmid-based cloning.
format Online
Article
Text
id pubmed-7566775
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Future Science Ltd
record_format MEDLINE/PubMed
spelling pubmed-75667752020-10-20 A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates Tossberg, John T Esmond, Tashawna M Aune, Thomas M Biotechniques Reports We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted luciferase, enhanced green fluorescence protein, IL-4, and IL-12A and IL-12B to form active IL-12. We propose that this method offers a cost- and time-saving alternative to plasmid-based cloning. Future Science Ltd 2020-08-20 2020-07 /pmc/articles/PMC7566775/ /pubmed/32815735 http://dx.doi.org/10.2144/btn-2020-0037 Text en © 2020 Thomas M. Aune This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Reports
Tossberg, John T
Esmond, Tashawna M
Aune, Thomas M
A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
title A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
title_full A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
title_fullStr A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
title_full_unstemmed A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
title_short A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
title_sort simplified method to produce mrnas and functional proteins from synthetic double-stranded dna templates
topic Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566775/
https://www.ncbi.nlm.nih.gov/pubmed/32815735
http://dx.doi.org/10.2144/btn-2020-0037
work_keys_str_mv AT tossbergjohnt asimplifiedmethodtoproducemrnasandfunctionalproteinsfromsyntheticdoublestrandeddnatemplates
AT esmondtashawnam asimplifiedmethodtoproducemrnasandfunctionalproteinsfromsyntheticdoublestrandeddnatemplates
AT aunethomasm asimplifiedmethodtoproducemrnasandfunctionalproteinsfromsyntheticdoublestrandeddnatemplates
AT tossbergjohnt simplifiedmethodtoproducemrnasandfunctionalproteinsfromsyntheticdoublestrandeddnatemplates
AT esmondtashawnam simplifiedmethodtoproducemrnasandfunctionalproteinsfromsyntheticdoublestrandeddnatemplates
AT aunethomasm simplifiedmethodtoproducemrnasandfunctionalproteinsfromsyntheticdoublestrandeddnatemplates

Ejemplares similares