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A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted lucifer...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Future Science Ltd
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566775/ https://www.ncbi.nlm.nih.gov/pubmed/32815735 http://dx.doi.org/10.2144/btn-2020-0037 |