Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform

Recombinant protein technology enables the engineering of modern vaccines composed of a carrier protein displaying poorly immunogenic heterologous antigens. One promising carrier is based on the rotavirus inner-capsid VP6 protein. We explored different VP6 insertion sites for the presentation of two...

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Autores principales: Gröhn, Stina, Heinimäki, Suvi, Tamminen, Kirsi, Blazevic, Vesna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567002/
https://www.ncbi.nlm.nih.gov/pubmed/33067651
http://dx.doi.org/10.1007/s00705-020-04847-5
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author Gröhn, Stina
Heinimäki, Suvi
Tamminen, Kirsi
Blazevic, Vesna
author_facet Gröhn, Stina
Heinimäki, Suvi
Tamminen, Kirsi
Blazevic, Vesna
author_sort Gröhn, Stina
collection PubMed
description Recombinant protein technology enables the engineering of modern vaccines composed of a carrier protein displaying poorly immunogenic heterologous antigens. One promising carrier is based on the rotavirus inner-capsid VP6 protein. We explored different VP6 insertion sites for the presentation of two peptides (23 and 140 amino acids) derived from the M2 and HA genes of influenza A virus. Both termini and three surface loops of VP6 were successfully exploited as genetic fusion sites, as demonstrated by the expression of the fusion proteins. However, further studies are needed to assess the morphology and immunogenicity of these constructs.
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spelling pubmed-75670022020-10-19 Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform Gröhn, Stina Heinimäki, Suvi Tamminen, Kirsi Blazevic, Vesna Arch Virol Brief Report Recombinant protein technology enables the engineering of modern vaccines composed of a carrier protein displaying poorly immunogenic heterologous antigens. One promising carrier is based on the rotavirus inner-capsid VP6 protein. We explored different VP6 insertion sites for the presentation of two peptides (23 and 140 amino acids) derived from the M2 and HA genes of influenza A virus. Both termini and three surface loops of VP6 were successfully exploited as genetic fusion sites, as demonstrated by the expression of the fusion proteins. However, further studies are needed to assess the morphology and immunogenicity of these constructs. Springer Vienna 2020-10-16 2021 /pmc/articles/PMC7567002/ /pubmed/33067651 http://dx.doi.org/10.1007/s00705-020-04847-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Report
Gröhn, Stina
Heinimäki, Suvi
Tamminen, Kirsi
Blazevic, Vesna
Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform
title Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform
title_full Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform
title_fullStr Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform
title_full_unstemmed Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform
title_short Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform
title_sort expression of influenza a virus-derived peptides on a rotavirus vp6-based delivery platform
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567002/
https://www.ncbi.nlm.nih.gov/pubmed/33067651
http://dx.doi.org/10.1007/s00705-020-04847-5
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