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Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time
Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genom...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567609/ https://www.ncbi.nlm.nih.gov/pubmed/32955440 http://dx.doi.org/10.7554/eLife.55308 |
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author | Ireland, William T Beeler, Suzannah M Flores-Bautista, Emanuel McCarty, Nicholas S Röschinger, Tom Belliveau, Nathan M Sweredoski, Michael J Moradian, Annie Kinney, Justin B Phillips, Rob |
author_facet | Ireland, William T Beeler, Suzannah M Flores-Bautista, Emanuel McCarty, Nicholas S Röschinger, Tom Belliveau, Nathan M Sweredoski, Michael J Moradian, Annie Kinney, Justin B Phillips, Rob |
author_sort | Ireland, William T |
collection | PubMed |
description | Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haphazard. We introduce a new method, Reg-Seq, that links massively parallel reporter assays with mass spectrometry to produce a base pair resolution dissection of more than a E. coli promoters in 12 growth conditions. We demonstrate that the method recapitulates known regulatory information. Then, we examine regulatory architectures for more than 80 promoters which previously had no known regulatory information. In many cases, we also identify which transcription factors mediate their regulation. This method clears a path for highly multiplexed investigations of the regulatory genome of model organisms, with the potential of moving to an array of microbes of ecological and medical relevance. |
format | Online Article Text |
id | pubmed-7567609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-75676092020-10-19 Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time Ireland, William T Beeler, Suzannah M Flores-Bautista, Emanuel McCarty, Nicholas S Röschinger, Tom Belliveau, Nathan M Sweredoski, Michael J Moradian, Annie Kinney, Justin B Phillips, Rob eLife Physics of Living Systems Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haphazard. We introduce a new method, Reg-Seq, that links massively parallel reporter assays with mass spectrometry to produce a base pair resolution dissection of more than a E. coli promoters in 12 growth conditions. We demonstrate that the method recapitulates known regulatory information. Then, we examine regulatory architectures for more than 80 promoters which previously had no known regulatory information. In many cases, we also identify which transcription factors mediate their regulation. This method clears a path for highly multiplexed investigations of the regulatory genome of model organisms, with the potential of moving to an array of microbes of ecological and medical relevance. eLife Sciences Publications, Ltd 2020-09-21 /pmc/articles/PMC7567609/ /pubmed/32955440 http://dx.doi.org/10.7554/eLife.55308 Text en © 2020, Ireland et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Physics of Living Systems Ireland, William T Beeler, Suzannah M Flores-Bautista, Emanuel McCarty, Nicholas S Röschinger, Tom Belliveau, Nathan M Sweredoski, Michael J Moradian, Annie Kinney, Justin B Phillips, Rob Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
title | Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
title_full | Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
title_fullStr | Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
title_full_unstemmed | Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
title_short | Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
title_sort | deciphering the regulatory genome of escherichia coli, one hundred promoters at a time |
topic | Physics of Living Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567609/ https://www.ncbi.nlm.nih.gov/pubmed/32955440 http://dx.doi.org/10.7554/eLife.55308 |
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