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The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research
Too many pre-clinical experiments are giving results which cannot be reproduced. This may be because the experiments are incorrectly designed. In “Completely randomized” (CR) and “Randomised block” (RB) experimental designs, both the assignment of treatments to experimental subjects and the order in...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567855/ https://www.ncbi.nlm.nih.gov/pubmed/33067494 http://dx.doi.org/10.1038/s41598-020-74538-3 |
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author | Festing, Michael F. W. |
author_facet | Festing, Michael F. W. |
author_sort | Festing, Michael F. W. |
collection | PubMed |
description | Too many pre-clinical experiments are giving results which cannot be reproduced. This may be because the experiments are incorrectly designed. In “Completely randomized” (CR) and “Randomised block” (RB) experimental designs, both the assignment of treatments to experimental subjects and the order in which the experiment is done, are randomly determined. These designs have been used successfully in agricultural and industrial research and in clinical trials for nearly a century without excessive levels of irreproducibility. They must also be used in pre-clinical research if the excessive level of irreproducibility is to be eliminated. A survey of 100 papers involving mice and rats was used to determine whether scientists had used the CR or RB designs. The papers were assigned to three categories “Design acceptable”, “Randomised to treatment groups”, so of doubtful validity, or “Room for improvement”. Only 32 ± 4.7% of the papers fell into the first group, although none of them actually named either the CR or RB design. If the current high level of irreproducibility is to be eliminated, it is essential that scientists engaged in pre-clinical research use “Completely randomised” (CR), “Randomised block” (RB), or one of the more specialised named experimental designs described in textbooks on the subject. |
format | Online Article Text |
id | pubmed-7567855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75678552020-10-19 The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research Festing, Michael F. W. Sci Rep Article Too many pre-clinical experiments are giving results which cannot be reproduced. This may be because the experiments are incorrectly designed. In “Completely randomized” (CR) and “Randomised block” (RB) experimental designs, both the assignment of treatments to experimental subjects and the order in which the experiment is done, are randomly determined. These designs have been used successfully in agricultural and industrial research and in clinical trials for nearly a century without excessive levels of irreproducibility. They must also be used in pre-clinical research if the excessive level of irreproducibility is to be eliminated. A survey of 100 papers involving mice and rats was used to determine whether scientists had used the CR or RB designs. The papers were assigned to three categories “Design acceptable”, “Randomised to treatment groups”, so of doubtful validity, or “Room for improvement”. Only 32 ± 4.7% of the papers fell into the first group, although none of them actually named either the CR or RB design. If the current high level of irreproducibility is to be eliminated, it is essential that scientists engaged in pre-clinical research use “Completely randomised” (CR), “Randomised block” (RB), or one of the more specialised named experimental designs described in textbooks on the subject. Nature Publishing Group UK 2020-10-16 /pmc/articles/PMC7567855/ /pubmed/33067494 http://dx.doi.org/10.1038/s41598-020-74538-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Festing, Michael F. W. The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
title | The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
title_full | The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
title_fullStr | The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
title_full_unstemmed | The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
title_short | The “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
title_sort | “completely randomised” and the “randomised block” are the only experimental designs suitable for widespread use in pre-clinical research |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567855/ https://www.ncbi.nlm.nih.gov/pubmed/33067494 http://dx.doi.org/10.1038/s41598-020-74538-3 |
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