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Characterization of stanniocalcin-1 expression in macrophage differentiation
Human stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. The role of STC1 in the pro- and anti-inflammatory functions of differentiating macrophage, however, is not clear. In this study, our data showed that phorbol 12-myristate 13-acetate (PMA) treatment i...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568195/ https://www.ncbi.nlm.nih.gov/pubmed/33074126 http://dx.doi.org/10.1016/j.tranon.2020.100881 |
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author | Leung, Cherry C.T. Wong, Chris K.C. |
author_facet | Leung, Cherry C.T. Wong, Chris K.C. |
author_sort | Leung, Cherry C.T. |
collection | PubMed |
description | Human stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. The role of STC1 in the pro- and anti-inflammatory functions of differentiating macrophage, however, is not clear. In this study, our data showed that phorbol 12-myristate 13-acetate (PMA) treatment induced human leukemia monocytic cells (ThP-1) differentiation to M0 macrophages. The differentiation was accompanied by a significant increase in the mRNA expression levels of STC1, the pro-inflammatory cytokine TNFα, and anti-inflammatory markers, CD163 & CD206. An intermitted removal of PMA treatment reduced the mRNA levels of STC1 and TNFα but had no noticeable effects on the anti-inflammatory markers. The correlation in the expression of STC1 and pro-inflammatory markers in differentiating macrophages was investigated, using siRNA(STC1)-transfected PMA-induced cells. Consistently, the transcripts levels of TNFα and IL-6 were significantly reduced. Moreover, LPS/IFNγ-induced M1-polarization showed remarkably higher expression levels of STC1 than IL-4/IL-13-induced M2-macrophages and PMA-induced M0-macrophages. Transcriptomic analysis of siRNA(STC1)-transfected M1-polarized cells revealed an upregulation of TBC1 domain family member 3 (TBC1D3G). The gene regulates the payload of macrophage-released extracellular vesicles to mediate inflammation. The conditioned media from siRNA(STC1)-transfected M1-polarized cells were found to reduce Hep3B cell motility. The data suggest that the expression of STC1 were associated with macrophage differentiation, but preferentially to M1 polarization. |
format | Online Article Text |
id | pubmed-7568195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-75681952020-10-22 Characterization of stanniocalcin-1 expression in macrophage differentiation Leung, Cherry C.T. Wong, Chris K.C. Transl Oncol Original article Human stanniocalcin-1 (STC1) is a paracrine factor associated with inflammation and carcinogenesis. The role of STC1 in the pro- and anti-inflammatory functions of differentiating macrophage, however, is not clear. In this study, our data showed that phorbol 12-myristate 13-acetate (PMA) treatment induced human leukemia monocytic cells (ThP-1) differentiation to M0 macrophages. The differentiation was accompanied by a significant increase in the mRNA expression levels of STC1, the pro-inflammatory cytokine TNFα, and anti-inflammatory markers, CD163 & CD206. An intermitted removal of PMA treatment reduced the mRNA levels of STC1 and TNFα but had no noticeable effects on the anti-inflammatory markers. The correlation in the expression of STC1 and pro-inflammatory markers in differentiating macrophages was investigated, using siRNA(STC1)-transfected PMA-induced cells. Consistently, the transcripts levels of TNFα and IL-6 were significantly reduced. Moreover, LPS/IFNγ-induced M1-polarization showed remarkably higher expression levels of STC1 than IL-4/IL-13-induced M2-macrophages and PMA-induced M0-macrophages. Transcriptomic analysis of siRNA(STC1)-transfected M1-polarized cells revealed an upregulation of TBC1 domain family member 3 (TBC1D3G). The gene regulates the payload of macrophage-released extracellular vesicles to mediate inflammation. The conditioned media from siRNA(STC1)-transfected M1-polarized cells were found to reduce Hep3B cell motility. The data suggest that the expression of STC1 were associated with macrophage differentiation, but preferentially to M1 polarization. Neoplasia Press 2020-10-16 /pmc/articles/PMC7568195/ /pubmed/33074126 http://dx.doi.org/10.1016/j.tranon.2020.100881 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article Leung, Cherry C.T. Wong, Chris K.C. Characterization of stanniocalcin-1 expression in macrophage differentiation |
title | Characterization of stanniocalcin-1 expression in macrophage differentiation |
title_full | Characterization of stanniocalcin-1 expression in macrophage differentiation |
title_fullStr | Characterization of stanniocalcin-1 expression in macrophage differentiation |
title_full_unstemmed | Characterization of stanniocalcin-1 expression in macrophage differentiation |
title_short | Characterization of stanniocalcin-1 expression in macrophage differentiation |
title_sort | characterization of stanniocalcin-1 expression in macrophage differentiation |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568195/ https://www.ncbi.nlm.nih.gov/pubmed/33074126 http://dx.doi.org/10.1016/j.tranon.2020.100881 |
work_keys_str_mv | AT leungcherryct characterizationofstanniocalcin1expressioninmacrophagedifferentiation AT wongchriskc characterizationofstanniocalcin1expressioninmacrophagedifferentiation |