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Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy
OBJECTIVES: Recent research shows that most high grade ovarian cancer (OC) originates from the fallopian tube (FT). Cytologic evaluation of FT cells may enable early detection of OC. MATERIAL AND METHODS: This was a prospective study with patients enrolled from 3 centers (October 2016– August 2017)....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Scientific Scholar
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568227/ https://www.ncbi.nlm.nih.gov/pubmed/33093855 http://dx.doi.org/10.25259/CytoJournal_7_2020 |
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author | Pramanik, Sharmila Yang, Eric Wu, Wendy |
author_facet | Pramanik, Sharmila Yang, Eric Wu, Wendy |
author_sort | Pramanik, Sharmila |
collection | PubMed |
description | OBJECTIVES: Recent research shows that most high grade ovarian cancer (OC) originates from the fallopian tube (FT). Cytologic evaluation of FT cells may enable early detection of OC. MATERIAL AND METHODS: This was a prospective study with patients enrolled from 3 centers (October 2016– August 2017). Forty-two women undergoing salpingo-oophorectomy for a pelvic mass suspicious for malignancy or undergoing risk-reducing surgery for BRCA mutations were included in the study. At the time of scheduled surgery, a novel catheter was used to collect FT cells through hysteroscopy. A pathologist blinded to surgical or pathologic findings evaluated FT cytology, and results were compared to pathology. RESULTS: Of the 61 samples collected, 72% (44/61) met the adequacy criteria (≥5 clusters of cells with 20 cells in each cluster). Cytology classification criteria were established and applied to adequate samples. Forty-four samples were benign with mixed population of cells with round, oval, and spindled nuclei; 2-dimensional clusters; columnar cell configuration; flat sheets; cilia presence; no/mild nuclear pleomorphism; no nuclear membrane irregularities; and no nucleoli. Five samples had benign features with reactive nuclear and cytoplasmic changes and/or background inflammation, which were categorized as “reactive atypia.” Two malignant samples had features of 3-dimensional (3D) clusters, loss of mixed population of cells; increased nuclear/cytoplasmic ratio; nuclear membrane irregularity and nucleoli presence. Three samples with some but not all of malignant features were categorized as “neoplastic” (anisonucleosis; small nucleoli and features suggestive of 3D clusters). Malignant/ neoplastic samples were labeled as “Positive” (n = 5) while benign/reactive samples were labeled as “Negative” (n = 39). A high concordance rate (95%, 42/44) was observed between cytology results and histology. CONCLUSIONS: We characterized cytologic features for pathologically distinct FT samples collected in vivo using a novel catheter and demonstrated its value in detecting OC. |
format | Online Article Text |
id | pubmed-7568227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Scientific Scholar |
record_format | MEDLINE/PubMed |
spelling | pubmed-75682272020-10-21 Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy Pramanik, Sharmila Yang, Eric Wu, Wendy Cytojournal Research Article OBJECTIVES: Recent research shows that most high grade ovarian cancer (OC) originates from the fallopian tube (FT). Cytologic evaluation of FT cells may enable early detection of OC. MATERIAL AND METHODS: This was a prospective study with patients enrolled from 3 centers (October 2016– August 2017). Forty-two women undergoing salpingo-oophorectomy for a pelvic mass suspicious for malignancy or undergoing risk-reducing surgery for BRCA mutations were included in the study. At the time of scheduled surgery, a novel catheter was used to collect FT cells through hysteroscopy. A pathologist blinded to surgical or pathologic findings evaluated FT cytology, and results were compared to pathology. RESULTS: Of the 61 samples collected, 72% (44/61) met the adequacy criteria (≥5 clusters of cells with 20 cells in each cluster). Cytology classification criteria were established and applied to adequate samples. Forty-four samples were benign with mixed population of cells with round, oval, and spindled nuclei; 2-dimensional clusters; columnar cell configuration; flat sheets; cilia presence; no/mild nuclear pleomorphism; no nuclear membrane irregularities; and no nucleoli. Five samples had benign features with reactive nuclear and cytoplasmic changes and/or background inflammation, which were categorized as “reactive atypia.” Two malignant samples had features of 3-dimensional (3D) clusters, loss of mixed population of cells; increased nuclear/cytoplasmic ratio; nuclear membrane irregularity and nucleoli presence. Three samples with some but not all of malignant features were categorized as “neoplastic” (anisonucleosis; small nucleoli and features suggestive of 3D clusters). Malignant/ neoplastic samples were labeled as “Positive” (n = 5) while benign/reactive samples were labeled as “Negative” (n = 39). A high concordance rate (95%, 42/44) was observed between cytology results and histology. CONCLUSIONS: We characterized cytologic features for pathologically distinct FT samples collected in vivo using a novel catheter and demonstrated its value in detecting OC. Scientific Scholar 2020-08-10 /pmc/articles/PMC7568227/ /pubmed/33093855 http://dx.doi.org/10.25259/CytoJournal_7_2020 Text en ©2020 Cytopathology Foundation Inc, Published by Scientific Scholar https://creativecommons.org/licenses/by-nc-sa/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial- Share Alike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Pramanik, Sharmila Yang, Eric Wu, Wendy Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
title | Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
title_full | Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
title_fullStr | Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
title_full_unstemmed | Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
title_short | Cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
title_sort | cytologic studies of in vivo fallopian tube specimens in patients undergoing salpingo-oophorectomy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568227/ https://www.ncbi.nlm.nih.gov/pubmed/33093855 http://dx.doi.org/10.25259/CytoJournal_7_2020 |
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