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Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets

BACKGROUND: Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of antibiotic exposure on skeletal muscle properties remain unknown. The present study evaluated the changes in skeletal muscle properties including myofiber characteristics and com...

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Autores principales: Yan, Honglin, Yu, Bing, Degroote, Jeroen, Spranghers, Thomas, Van Noten, Noémie, Majdeddin, Maryam, Van Poucke, Mario, Peelman, Luc, De Vrieze, Jo, Boon, Nico, Gielen, Ingrid, Smet, Stefaan De, Chen, Daiwen, Michiels, Joris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568366/
https://www.ncbi.nlm.nih.gov/pubmed/33066774
http://dx.doi.org/10.1186/s12917-020-02592-0
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author Yan, Honglin
Yu, Bing
Degroote, Jeroen
Spranghers, Thomas
Van Noten, Noémie
Majdeddin, Maryam
Van Poucke, Mario
Peelman, Luc
De Vrieze, Jo
Boon, Nico
Gielen, Ingrid
Smet, Stefaan De
Chen, Daiwen
Michiels, Joris
author_facet Yan, Honglin
Yu, Bing
Degroote, Jeroen
Spranghers, Thomas
Van Noten, Noémie
Majdeddin, Maryam
Van Poucke, Mario
Peelman, Luc
De Vrieze, Jo
Boon, Nico
Gielen, Ingrid
Smet, Stefaan De
Chen, Daiwen
Michiels, Joris
author_sort Yan, Honglin
collection PubMed
description BACKGROUND: Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of antibiotic exposure on skeletal muscle properties remain unknown. The present study evaluated the changes in skeletal muscle properties including myofiber characteristics and composition, as well as intramuscular fat (IMF) content in skeletal muscle of piglets when exposed to a tylosin-containing diet. RESULTS: A total of 18 piglets (28 days of age) were randomly allocated into two groups: control basal diet (Control) and Control + 100 mg tylosin phosphate/kg of feed (Antibiotic). The trial lasted for 39 days. High-throughput amplicon sequencing revealed that no significant difference in initial gut microbiota composition was existed between Control and Antibiotic groups. Antibiotic administration increased body weight and growth rate and decreased feed to gain ratio of pigs (P < 0.05). The carcass lean and fat volumes of pigs were increased by the tylosin administration (P < 0.05). Antibiotic treatment increased myofiber density and the expression of genes related to type I and type IIb myofibers in longissimus muscle (P < 0.05). The IMF content in longissimus muscle was increased by antibiotic exposure (P < 0.05). Antibiotic administration increased expression of genes related to fatty acid uptake and de novo synthesis, and decreased expression of genes related to triglyceride hydrolysis (P < 0.05). Tylosin administration affected taxonomic distribution and beta diversity of the caecal and colonic microbiota of piglets. CONCLUSION: These results confirm that the growth performance, myofiber composition and muscle lipid metabolism are affected by antibiotic administration, which may be associated with an altered gut microbiota, suggesting that the gut microbiota could be served as a potential target for modulating skeletal muscle properties of host.
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spelling pubmed-75683662020-10-20 Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets Yan, Honglin Yu, Bing Degroote, Jeroen Spranghers, Thomas Van Noten, Noémie Majdeddin, Maryam Van Poucke, Mario Peelman, Luc De Vrieze, Jo Boon, Nico Gielen, Ingrid Smet, Stefaan De Chen, Daiwen Michiels, Joris BMC Vet Res Research Article BACKGROUND: Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of antibiotic exposure on skeletal muscle properties remain unknown. The present study evaluated the changes in skeletal muscle properties including myofiber characteristics and composition, as well as intramuscular fat (IMF) content in skeletal muscle of piglets when exposed to a tylosin-containing diet. RESULTS: A total of 18 piglets (28 days of age) were randomly allocated into two groups: control basal diet (Control) and Control + 100 mg tylosin phosphate/kg of feed (Antibiotic). The trial lasted for 39 days. High-throughput amplicon sequencing revealed that no significant difference in initial gut microbiota composition was existed between Control and Antibiotic groups. Antibiotic administration increased body weight and growth rate and decreased feed to gain ratio of pigs (P < 0.05). The carcass lean and fat volumes of pigs were increased by the tylosin administration (P < 0.05). Antibiotic treatment increased myofiber density and the expression of genes related to type I and type IIb myofibers in longissimus muscle (P < 0.05). The IMF content in longissimus muscle was increased by antibiotic exposure (P < 0.05). Antibiotic administration increased expression of genes related to fatty acid uptake and de novo synthesis, and decreased expression of genes related to triglyceride hydrolysis (P < 0.05). Tylosin administration affected taxonomic distribution and beta diversity of the caecal and colonic microbiota of piglets. CONCLUSION: These results confirm that the growth performance, myofiber composition and muscle lipid metabolism are affected by antibiotic administration, which may be associated with an altered gut microbiota, suggesting that the gut microbiota could be served as a potential target for modulating skeletal muscle properties of host. BioMed Central 2020-10-16 /pmc/articles/PMC7568366/ /pubmed/33066774 http://dx.doi.org/10.1186/s12917-020-02592-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Yan, Honglin
Yu, Bing
Degroote, Jeroen
Spranghers, Thomas
Van Noten, Noémie
Majdeddin, Maryam
Van Poucke, Mario
Peelman, Luc
De Vrieze, Jo
Boon, Nico
Gielen, Ingrid
Smet, Stefaan De
Chen, Daiwen
Michiels, Joris
Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
title Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
title_full Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
title_fullStr Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
title_full_unstemmed Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
title_short Antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
title_sort antibiotic affects the gut microbiota composition and expression of genes related to lipid metabolism and myofiber types in skeletal muscle of piglets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568366/
https://www.ncbi.nlm.nih.gov/pubmed/33066774
http://dx.doi.org/10.1186/s12917-020-02592-0
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