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Improved thermostability of creatinase from Alcaligenes Faecalis through non-biased phylogenetic consensus-guided mutagenesis

BACKGROUND: Enzymatic quantification of creatinine has become an essential method for clinical evaluation of renal function. Although creatinase (CR) is frequently used for this purpose, its poor thermostability severely limits industrial applications. Herein, we report a novel creatinase from Alcal...

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Detalles Bibliográficos
Autores principales:	Bai, Xue, Li, Daixi, Ma, Fuqiang, Deng, Xi, Luo, Manjie, Feng, Yan, Yang, Guangyu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568399/ https://www.ncbi.nlm.nih.gov/pubmed/33069232 http://dx.doi.org/10.1186/s12934-020-01451-9

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