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Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats
Hydrogen sulfide (H(2)S) is constitutively generated in the human body and works as a gasotransmitter in synaptic transmission. In this study, we aimed to evaluate the roles of endogenous H(2)S in generating eupnea at the respiratory center. We employed an in situ arterially perfused preparation of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568547/ https://www.ncbi.nlm.nih.gov/pubmed/33067579 http://dx.doi.org/10.1038/s42003-020-01312-6 |
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author | Okazaki, Minako Uozu, Saori Sato, Yuma Matsumoto, Masayuki Koganezawa, Tadachika |
author_facet | Okazaki, Minako Uozu, Saori Sato, Yuma Matsumoto, Masayuki Koganezawa, Tadachika |
author_sort | Okazaki, Minako |
collection | PubMed |
description | Hydrogen sulfide (H(2)S) is constitutively generated in the human body and works as a gasotransmitter in synaptic transmission. In this study, we aimed to evaluate the roles of endogenous H(2)S in generating eupnea at the respiratory center. We employed an in situ arterially perfused preparation of decerebrated rats and recorded the central respiratory outputs. When the H(2)S-producing enzyme cystathionine β-synthase (CBS) was inhibited, respiration switched from the 3-phase eupneic pattern, which consists of inspiration, postinspiration, and expiration, to gasping-like respiration, which consists of inspiration only. On the other hand, when H(2)S synthesis was inhibited via cystathionine γ-lyase (CSE) or when H(2)S synthesis was activated via CBS, eupnea remained unchanged. These results suggest that H(2)S produced by CBS has crucial roles in maintaining the neuronal network to generate eupnea. The mechanism of respiratory pattern generation might be switched from a network-based system to a pacemaker cell-based system in low H(2)S conditions. |
format | Online Article Text |
id | pubmed-7568547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75685472020-10-20 Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats Okazaki, Minako Uozu, Saori Sato, Yuma Matsumoto, Masayuki Koganezawa, Tadachika Commun Biol Article Hydrogen sulfide (H(2)S) is constitutively generated in the human body and works as a gasotransmitter in synaptic transmission. In this study, we aimed to evaluate the roles of endogenous H(2)S in generating eupnea at the respiratory center. We employed an in situ arterially perfused preparation of decerebrated rats and recorded the central respiratory outputs. When the H(2)S-producing enzyme cystathionine β-synthase (CBS) was inhibited, respiration switched from the 3-phase eupneic pattern, which consists of inspiration, postinspiration, and expiration, to gasping-like respiration, which consists of inspiration only. On the other hand, when H(2)S synthesis was inhibited via cystathionine γ-lyase (CSE) or when H(2)S synthesis was activated via CBS, eupnea remained unchanged. These results suggest that H(2)S produced by CBS has crucial roles in maintaining the neuronal network to generate eupnea. The mechanism of respiratory pattern generation might be switched from a network-based system to a pacemaker cell-based system in low H(2)S conditions. Nature Publishing Group UK 2020-10-16 /pmc/articles/PMC7568547/ /pubmed/33067579 http://dx.doi.org/10.1038/s42003-020-01312-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Okazaki, Minako Uozu, Saori Sato, Yuma Matsumoto, Masayuki Koganezawa, Tadachika Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
title | Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
title_full | Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
title_fullStr | Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
title_full_unstemmed | Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
title_short | Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
title_sort | endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568547/ https://www.ncbi.nlm.nih.gov/pubmed/33067579 http://dx.doi.org/10.1038/s42003-020-01312-6 |
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