Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells

OBJECTIVE: The purpose of this study was to prepare and characterize a lipid magnetic ball modified with KRAS antibodies on the surface and to isolate circulating tumor cells of colorectal cancer with KRAS mutations. METHODS: The microemulsion method was used to form lipid bilayers to encapsulate Fe...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Chengbao, Wang, Jingjing, Yang, Xue, Zang, Xuefang, Zhou, Haikun, Zhang, Erying, Li, Haifei, Liu, Bo, Chen, Shumin, Wang, Ying, Wang, Peng, Gao, Lixue, Liu, Jinyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568589/
https://www.ncbi.nlm.nih.gov/pubmed/33116863
http://dx.doi.org/10.2147/CMAR.S258565
_version_ 1783596552492679168
author Feng, Chengbao
Wang, Jingjing
Yang, Xue
Zang, Xuefang
Zhou, Haikun
Zhang, Erying
Li, Haifei
Liu, Bo
Chen, Shumin
Wang, Ying
Wang, Peng
Gao, Lixue
Liu, Jinyan
author_facet Feng, Chengbao
Wang, Jingjing
Yang, Xue
Zang, Xuefang
Zhou, Haikun
Zhang, Erying
Li, Haifei
Liu, Bo
Chen, Shumin
Wang, Ying
Wang, Peng
Gao, Lixue
Liu, Jinyan
author_sort Feng, Chengbao
collection PubMed
description OBJECTIVE: The purpose of this study was to prepare and characterize a lipid magnetic ball modified with KRAS antibodies on the surface and to isolate circulating tumor cells of colorectal cancer with KRAS mutations. METHODS: The microemulsion method was used to form lipid bilayers to encapsulate Fe3O4 nanoparticles with superparamagnetism to form lipid magnetic balls, and KRAS antibodies were formed on the surface to form KRAS immune lipid magnetic balls. RESULTS: Compared with traditional EpCAM antibody-modified lipid magnetic balls, it can effectively improve the capture ability of colorectal cancer circulating tumor cells with KRAS mutation, the capture rate reaches 92.9%, and the capture results are consistent with clinical diagnosis and pathology. CONCLUSION: Our results showed that KRAS antibody-modified lipid magnetic balls can be used in the diagnosis and treatment of KRAS colorectal cancer.
format Online
Article
Text
id pubmed-7568589
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-75685892020-10-27 Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells Feng, Chengbao Wang, Jingjing Yang, Xue Zang, Xuefang Zhou, Haikun Zhang, Erying Li, Haifei Liu, Bo Chen, Shumin Wang, Ying Wang, Peng Gao, Lixue Liu, Jinyan Cancer Manag Res Original Research OBJECTIVE: The purpose of this study was to prepare and characterize a lipid magnetic ball modified with KRAS antibodies on the surface and to isolate circulating tumor cells of colorectal cancer with KRAS mutations. METHODS: The microemulsion method was used to form lipid bilayers to encapsulate Fe3O4 nanoparticles with superparamagnetism to form lipid magnetic balls, and KRAS antibodies were formed on the surface to form KRAS immune lipid magnetic balls. RESULTS: Compared with traditional EpCAM antibody-modified lipid magnetic balls, it can effectively improve the capture ability of colorectal cancer circulating tumor cells with KRAS mutation, the capture rate reaches 92.9%, and the capture results are consistent with clinical diagnosis and pathology. CONCLUSION: Our results showed that KRAS antibody-modified lipid magnetic balls can be used in the diagnosis and treatment of KRAS colorectal cancer. Dove 2020-10-13 /pmc/articles/PMC7568589/ /pubmed/33116863 http://dx.doi.org/10.2147/CMAR.S258565 Text en © 2020 Feng et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Feng, Chengbao
Wang, Jingjing
Yang, Xue
Zang, Xuefang
Zhou, Haikun
Zhang, Erying
Li, Haifei
Liu, Bo
Chen, Shumin
Wang, Ying
Wang, Peng
Gao, Lixue
Liu, Jinyan
Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells
title Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells
title_full Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells
title_fullStr Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells
title_full_unstemmed Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells
title_short Construction and Characterization of KRAS Immune Lipid Magnetic Balls for Colorectal Cancer Circulating Tumor Cells
title_sort construction and characterization of kras immune lipid magnetic balls for colorectal cancer circulating tumor cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568589/
https://www.ncbi.nlm.nih.gov/pubmed/33116863
http://dx.doi.org/10.2147/CMAR.S258565
work_keys_str_mv AT fengchengbao constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT wangjingjing constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT yangxue constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT zangxuefang constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT zhouhaikun constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT zhangerying constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT lihaifei constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT liubo constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT chenshumin constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT wangying constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT wangpeng constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT gaolixue constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells
AT liujinyan constructionandcharacterizationofkrasimmunelipidmagneticballsforcolorectalcancercirculatingtumorcells

Ejemplares similares