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Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea

PURPOSE: The emergence of Neisseria gonorrhoeae strains that are resistant to the most commonly used antibiotics represents a great concern for global public health. This challenges the effectiveness of clinical treatment regimens and demands the development of alternative antigonococcal agent. In t...

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Autores principales: Alqahtani, Fulwah, Aleanizy, Fadilah, El Tahir, Eram, Alhabib, Hiba, Alsaif, Raghad, Shazly, Gamal, AlQahtani, Hajar, Alsarra, Ibrahim, Mahdavi, Jafar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568623/
https://www.ncbi.nlm.nih.gov/pubmed/33116506
http://dx.doi.org/10.2147/IJN.S272736
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author Alqahtani, Fulwah
Aleanizy, Fadilah
El Tahir, Eram
Alhabib, Hiba
Alsaif, Raghad
Shazly, Gamal
AlQahtani, Hajar
Alsarra, Ibrahim
Mahdavi, Jafar
author_facet Alqahtani, Fulwah
Aleanizy, Fadilah
El Tahir, Eram
Alhabib, Hiba
Alsaif, Raghad
Shazly, Gamal
AlQahtani, Hajar
Alsarra, Ibrahim
Mahdavi, Jafar
author_sort Alqahtani, Fulwah
collection PubMed
description PURPOSE: The emergence of Neisseria gonorrhoeae strains that are resistant to the most commonly used antibiotics represents a great concern for global public health. This challenges the effectiveness of clinical treatment regimens and demands the development of alternative antigonococcal agent. In this regard, chitosan nanoparticles (CNPs) are known to have antimicrobial activity against a wide range of pathogens. Thus, they have become a potential candidate for combatting this era of multi-drug resistance. This study aims to formulate CNPs, characterize their physicochemical properties, and examine their antimicrobial activity against gonococcus. MATERIALS AND METHODS: The ionic gelation method was used to prepare CNPs of different concentrations. Characterization for their particle size (PZ), polydispersity index (PDI), and zeta potential (ZP) was performed. The anti-microbial activity of CNPs was investigated against 13 WHO N. gonorrhoeae reference strains, using the broth dilution method. Cytotoxicity of CNPs and their effect on bacterial adhesion to HeLa cells were investigated. RESULTS: The average PZ and ZP of the prepared NPs were increased when the concentration of chitosan was increased from 1 to 5 mg/mL and found to be in the range of 193 nm ± 1.9 to 530 nm ± 13.3, and 14 mV ± 0.5 to 20 mV ± 1, respectively. Transmission electron microscopes (TEM) images revealed spherical NPs, and the NPs had a low PDI value of ≤0.27. The formed CNPs produced antibacterial activity against all tested strains, including those resistant to multiple antibiotics, with a minimum inhibitory concentration (MIC(90)) of 0.16 to 0.31 mg/mL and a minimum bactericidal concentration (MBC) of 0.31 to 0.61 mg/mL. Of note, at all MIC(90) and MBC, the CNPs had no significant cytotoxic effect on HeLa cells and reduced bacterial adhesion to these cells at MBC doses. CONCLUSION: The present work findings suggest the potential of the CNPs for the treatment of gonorrhoea.
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spelling pubmed-75686232020-10-27 Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea Alqahtani, Fulwah Aleanizy, Fadilah El Tahir, Eram Alhabib, Hiba Alsaif, Raghad Shazly, Gamal AlQahtani, Hajar Alsarra, Ibrahim Mahdavi, Jafar Int J Nanomedicine Original Research PURPOSE: The emergence of Neisseria gonorrhoeae strains that are resistant to the most commonly used antibiotics represents a great concern for global public health. This challenges the effectiveness of clinical treatment regimens and demands the development of alternative antigonococcal agent. In this regard, chitosan nanoparticles (CNPs) are known to have antimicrobial activity against a wide range of pathogens. Thus, they have become a potential candidate for combatting this era of multi-drug resistance. This study aims to formulate CNPs, characterize their physicochemical properties, and examine their antimicrobial activity against gonococcus. MATERIALS AND METHODS: The ionic gelation method was used to prepare CNPs of different concentrations. Characterization for their particle size (PZ), polydispersity index (PDI), and zeta potential (ZP) was performed. The anti-microbial activity of CNPs was investigated against 13 WHO N. gonorrhoeae reference strains, using the broth dilution method. Cytotoxicity of CNPs and their effect on bacterial adhesion to HeLa cells were investigated. RESULTS: The average PZ and ZP of the prepared NPs were increased when the concentration of chitosan was increased from 1 to 5 mg/mL and found to be in the range of 193 nm ± 1.9 to 530 nm ± 13.3, and 14 mV ± 0.5 to 20 mV ± 1, respectively. Transmission electron microscopes (TEM) images revealed spherical NPs, and the NPs had a low PDI value of ≤0.27. The formed CNPs produced antibacterial activity against all tested strains, including those resistant to multiple antibiotics, with a minimum inhibitory concentration (MIC(90)) of 0.16 to 0.31 mg/mL and a minimum bactericidal concentration (MBC) of 0.31 to 0.61 mg/mL. Of note, at all MIC(90) and MBC, the CNPs had no significant cytotoxic effect on HeLa cells and reduced bacterial adhesion to these cells at MBC doses. CONCLUSION: The present work findings suggest the potential of the CNPs for the treatment of gonorrhoea. Dove 2020-10-13 /pmc/articles/PMC7568623/ /pubmed/33116506 http://dx.doi.org/10.2147/IJN.S272736 Text en © 2020 Alqahtani et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Alqahtani, Fulwah
Aleanizy, Fadilah
El Tahir, Eram
Alhabib, Hiba
Alsaif, Raghad
Shazly, Gamal
AlQahtani, Hajar
Alsarra, Ibrahim
Mahdavi, Jafar
Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_full Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_fullStr Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_full_unstemmed Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_short Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_sort antibacterial activity of chitosan nanoparticles against pathogenic n. gonorrhoea
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568623/
https://www.ncbi.nlm.nih.gov/pubmed/33116506
http://dx.doi.org/10.2147/IJN.S272736
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