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Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes
The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We investigated the live interacti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568703/ https://www.ncbi.nlm.nih.gov/pubmed/32880037 http://dx.doi.org/10.1007/s00430-020-00691-1 |
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author | Alhede, Maria Lorenz, Melanie Fritz, Blaine Gabriel Jensen, Peter Østrup Ring, Hans Christian Bay, Lene Bjarnsholt, Thomas |
author_facet | Alhede, Maria Lorenz, Melanie Fritz, Blaine Gabriel Jensen, Peter Østrup Ring, Hans Christian Bay, Lene Bjarnsholt, Thomas |
author_sort | Alhede, Maria |
collection | PubMed |
description | The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We investigated the live interaction between PMNs and Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis using confocal scanning laser microscopy. Aggregate size significantly affected phagocytosis outcome and larger aggregates were less likely to be phagocytized. Aggregates of S. epidermidis were also less likely to be phagocytized than equally-sized aggregates of the other three species. We found that only aggregates of approx. 5 μm diameter or smaller were consistently phagocytosed. We demonstrate that planktonic and aggregated cells of all four species significantly reduced the viability of PMNs after 4 h of incubation. Our results indicate that larger bacterial aggregates are less likely to be phagocytosed by PMNs and we propose that, if the aggregates become too large, circulating PMNs may not be able to phagocytose them quickly enough, which may lead to chronic infection. |
format | Online Article Text |
id | pubmed-7568703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-75687032020-10-19 Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes Alhede, Maria Lorenz, Melanie Fritz, Blaine Gabriel Jensen, Peter Østrup Ring, Hans Christian Bay, Lene Bjarnsholt, Thomas Med Microbiol Immunol Original Investigation The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We investigated the live interaction between PMNs and Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis using confocal scanning laser microscopy. Aggregate size significantly affected phagocytosis outcome and larger aggregates were less likely to be phagocytized. Aggregates of S. epidermidis were also less likely to be phagocytized than equally-sized aggregates of the other three species. We found that only aggregates of approx. 5 μm diameter or smaller were consistently phagocytosed. We demonstrate that planktonic and aggregated cells of all four species significantly reduced the viability of PMNs after 4 h of incubation. Our results indicate that larger bacterial aggregates are less likely to be phagocytosed by PMNs and we propose that, if the aggregates become too large, circulating PMNs may not be able to phagocytose them quickly enough, which may lead to chronic infection. Springer Berlin Heidelberg 2020-09-02 2020 /pmc/articles/PMC7568703/ /pubmed/32880037 http://dx.doi.org/10.1007/s00430-020-00691-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Investigation Alhede, Maria Lorenz, Melanie Fritz, Blaine Gabriel Jensen, Peter Østrup Ring, Hans Christian Bay, Lene Bjarnsholt, Thomas Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
title | Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
title_full | Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
title_fullStr | Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
title_full_unstemmed | Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
title_short | Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
title_sort | bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes |
topic | Original Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568703/ https://www.ncbi.nlm.nih.gov/pubmed/32880037 http://dx.doi.org/10.1007/s00430-020-00691-1 |
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