Nexus between light and culture media on morphogenesis in Bacopa monnieri and saponin yield thereof

Bacopa monnieri, a well-documented nootropic plant of high commercial global demand had been explored for its effect in alleviating other diseases and symptoms. This is primarily attributed to different phytocompounds present in the plant. One of the major constituents among them are saponins. However, variation in agro-climatic conditions and choice of germplasm often affect the growth rate and yield of phytocompounds that significantly impact the efficacy of the plant and its extract. Tissue culture has been attempted to improve the yield of phytocompounds but is often restricted by higher cost and scalability. Current study explores the role of commercial hydroponic media ‘Leafy 200’ vis-à-vis Murashige and Skoog (MS) media, under different color and intensity of lights, on plant morphogenesis, biomass and saponin yield. Blue light induced more shoot differentiation than normal white light. Statistical studies performed using fractional factorial design showed no significant variations in the yield of saponins among the extracts. The study suggests that hydroponic culture to be a sustainable solution and possible substitute to tissue culture that may be exploited for scalable cultivation of the plant.