Cargando…

Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain

Here, we report a high quality annotated draft genome of Serratia marcescens 39_H1, a Gram-negative facultative anaerobe that was isolated from an anaerobic digester. The strain exhibited hydrolytic/acidogenic properties by significantly improving methane production when used as a single isolate ino...

Descripción completa

Detalles Bibliográficos
Autores principales: Obi, Linda U., Tekere, Memory, Roopnarain, Ashira, Sanko, Tomasz, Maguvu, Tawanda E., Bezuidenhout, Cornelius C., Adeleke, Rasheed A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569290/
https://www.ncbi.nlm.nih.gov/pubmed/33102161
http://dx.doi.org/10.1016/j.btre.2020.e00542
_version_ 1783596701274079232
author Obi, Linda U.
Tekere, Memory
Roopnarain, Ashira
Sanko, Tomasz
Maguvu, Tawanda E.
Bezuidenhout, Cornelius C.
Adeleke, Rasheed A.
author_facet Obi, Linda U.
Tekere, Memory
Roopnarain, Ashira
Sanko, Tomasz
Maguvu, Tawanda E.
Bezuidenhout, Cornelius C.
Adeleke, Rasheed A.
author_sort Obi, Linda U.
collection PubMed
description Here, we report a high quality annotated draft genome of Serratia marcescens 39_H1, a Gram-negative facultative anaerobe that was isolated from an anaerobic digester. The strain exhibited hydrolytic/acidogenic properties by significantly improving methane production when used as a single isolate inoculum during anaerobic digestion of water hyacinth and cow dung. The total genome size of the isolate was 5,106,712 bp which corresponds to an N50 of 267,528 and G + C content of 59.7 %. Genome annotation with the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) predicted a total of 4,908 genes of which 4,755 were protein coding genes; there were no plasmids detected. A number of genes associated with hydrolytic/acidogenic activities as well as other metabolic activities were identified and discussed.
format Online
Article
Text
id pubmed-7569290
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75692902020-10-22 Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain Obi, Linda U. Tekere, Memory Roopnarain, Ashira Sanko, Tomasz Maguvu, Tawanda E. Bezuidenhout, Cornelius C. Adeleke, Rasheed A. Biotechnol Rep (Amst) Research Article Here, we report a high quality annotated draft genome of Serratia marcescens 39_H1, a Gram-negative facultative anaerobe that was isolated from an anaerobic digester. The strain exhibited hydrolytic/acidogenic properties by significantly improving methane production when used as a single isolate inoculum during anaerobic digestion of water hyacinth and cow dung. The total genome size of the isolate was 5,106,712 bp which corresponds to an N50 of 267,528 and G + C content of 59.7 %. Genome annotation with the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) predicted a total of 4,908 genes of which 4,755 were protein coding genes; there were no plasmids detected. A number of genes associated with hydrolytic/acidogenic activities as well as other metabolic activities were identified and discussed. Elsevier 2020-10-09 /pmc/articles/PMC7569290/ /pubmed/33102161 http://dx.doi.org/10.1016/j.btre.2020.e00542 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Obi, Linda U.
Tekere, Memory
Roopnarain, Ashira
Sanko, Tomasz
Maguvu, Tawanda E.
Bezuidenhout, Cornelius C.
Adeleke, Rasheed A.
Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain
title Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain
title_full Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain
title_fullStr Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain
title_full_unstemmed Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain
title_short Whole genome sequence of Serratia marcescens 39_H1, a potential hydrolytic and acidogenic strain
title_sort whole genome sequence of serratia marcescens 39_h1, a potential hydrolytic and acidogenic strain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569290/
https://www.ncbi.nlm.nih.gov/pubmed/33102161
http://dx.doi.org/10.1016/j.btre.2020.e00542
work_keys_str_mv AT obilindau wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain
AT tekerememory wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain
AT roopnarainashira wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain
AT sankotomasz wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain
AT maguvutawandae wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain
AT bezuidenhoutcorneliusc wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain
AT adelekerasheeda wholegenomesequenceofserratiamarcescens39h1apotentialhydrolyticandacidogenicstrain