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Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study

BACKGROUND: Inflammation plays a key role in the aetiology and progression of Alzheimer’s disease (AD). However, the immunophenotype of the second most common neurodegenerative cause of dementia, dementia with Lewy bodies (DLB), remains unclear. To date there have been no studies examining periphera...

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Autores principales: Amin, Jay, Boche, Delphine, Clough, Zoe, Teeling, Jessica, Williams, Anthony, Gao, Yifang, Chudley, Lindsey, Lau, Laurie, Smith, Florence, Harris, Scott, Holmes, Clive
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569376/
https://www.ncbi.nlm.nih.gov/pubmed/32968032
http://dx.doi.org/10.1136/jnnp-2020-323603
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author Amin, Jay
Boche, Delphine
Clough, Zoe
Teeling, Jessica
Williams, Anthony
Gao, Yifang
Chudley, Lindsey
Lau, Laurie
Smith, Florence
Harris, Scott
Holmes, Clive
author_facet Amin, Jay
Boche, Delphine
Clough, Zoe
Teeling, Jessica
Williams, Anthony
Gao, Yifang
Chudley, Lindsey
Lau, Laurie
Smith, Florence
Harris, Scott
Holmes, Clive
author_sort Amin, Jay
collection PubMed
description BACKGROUND: Inflammation plays a key role in the aetiology and progression of Alzheimer’s disease (AD). However, the immunophenotype of the second most common neurodegenerative cause of dementia, dementia with Lewy bodies (DLB), remains unclear. To date there have been no studies examining peripheral inflammation in DLB using multiplex immunoassay and flow cytometry concomitantly. We hypothesised that, using blood biomarkers, DLB would show an increased proinflammatory profile compared with controls, and that there would be a distinct profile compared with AD. METHODS: 93 participants (31 with DLB, 31 with AD and 31 healthy older controls) completed a single study visit for neuropsychiatric testing and phlebotomy. Peripheral blood mononuclear cells were quantified for T and B cell subsets using flow cytometry, and serum cytokine concentrations were measured using multiplex immunoassay. RESULTS: We detected reduced relative numbers of helper T cells and reduced activation of B cells in DLB compared with AD. Additionally, interleukin (IL)-1β was detected more frequently in DLB and the serum concentration of IL-6 was increased compared with controls. CONCLUSIONS: Peripheral inflammation is altered in DLB compared with AD, with T cell subset analysis supporting a possible shift towards senescence of the adaptive immune system in DLB. Furthermore, there is a proinflammatory signature of serum cytokines in DLB. Identification of this unique peripheral immunophenotype in DLB could guide development of an immune-based biomarker and direct future work exploring potential immune modulation as a novel treatment.
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spelling pubmed-75693762020-10-20 Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study Amin, Jay Boche, Delphine Clough, Zoe Teeling, Jessica Williams, Anthony Gao, Yifang Chudley, Lindsey Lau, Laurie Smith, Florence Harris, Scott Holmes, Clive J Neurol Neurosurg Psychiatry Neuro-Inflammation BACKGROUND: Inflammation plays a key role in the aetiology and progression of Alzheimer’s disease (AD). However, the immunophenotype of the second most common neurodegenerative cause of dementia, dementia with Lewy bodies (DLB), remains unclear. To date there have been no studies examining peripheral inflammation in DLB using multiplex immunoassay and flow cytometry concomitantly. We hypothesised that, using blood biomarkers, DLB would show an increased proinflammatory profile compared with controls, and that there would be a distinct profile compared with AD. METHODS: 93 participants (31 with DLB, 31 with AD and 31 healthy older controls) completed a single study visit for neuropsychiatric testing and phlebotomy. Peripheral blood mononuclear cells were quantified for T and B cell subsets using flow cytometry, and serum cytokine concentrations were measured using multiplex immunoassay. RESULTS: We detected reduced relative numbers of helper T cells and reduced activation of B cells in DLB compared with AD. Additionally, interleukin (IL)-1β was detected more frequently in DLB and the serum concentration of IL-6 was increased compared with controls. CONCLUSIONS: Peripheral inflammation is altered in DLB compared with AD, with T cell subset analysis supporting a possible shift towards senescence of the adaptive immune system in DLB. Furthermore, there is a proinflammatory signature of serum cytokines in DLB. Identification of this unique peripheral immunophenotype in DLB could guide development of an immune-based biomarker and direct future work exploring potential immune modulation as a novel treatment. BMJ Publishing Group 2020-11 2020-09-23 /pmc/articles/PMC7569376/ /pubmed/32968032 http://dx.doi.org/10.1136/jnnp-2020-323603 Text en © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJ. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.
spellingShingle Neuro-Inflammation
Amin, Jay
Boche, Delphine
Clough, Zoe
Teeling, Jessica
Williams, Anthony
Gao, Yifang
Chudley, Lindsey
Lau, Laurie
Smith, Florence
Harris, Scott
Holmes, Clive
Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
title Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
title_full Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
title_fullStr Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
title_full_unstemmed Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
title_short Peripheral immunophenotype in dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
title_sort peripheral immunophenotype in dementia with lewy bodies and alzheimer’s disease: an observational clinical study
topic Neuro-Inflammation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569376/
https://www.ncbi.nlm.nih.gov/pubmed/32968032
http://dx.doi.org/10.1136/jnnp-2020-323603
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