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DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma

BACKGROUND: Identification of new biomarkers can facilitate the development of effective therapeutic strategies in breast cancer (BC). Data from previous studies have revealed that differentiated embryonic chondrocyte gene (DEC) 1 and DEC2 might involve in the progression of various cancer types. We...

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Autores principales: Fang, Wentong, Li, Qian, Wang, Min, Zheng, Mingjie, Xu, Huirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569433/
https://www.ncbi.nlm.nih.gov/pubmed/33101542
http://dx.doi.org/10.1155/2020/6053154
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author Fang, Wentong
Li, Qian
Wang, Min
Zheng, Mingjie
Xu, Huirong
author_facet Fang, Wentong
Li, Qian
Wang, Min
Zheng, Mingjie
Xu, Huirong
author_sort Fang, Wentong
collection PubMed
description BACKGROUND: Identification of new biomarkers can facilitate the development of effective therapeutic strategies in breast cancer (BC). Data from previous studies have revealed that differentiated embryonic chondrocyte gene (DEC) 1 and DEC2 might involve in the progression of various cancer types. We explored the expression profiles and function of DEC1/2 in BC patients in this study. METHODS: The mRNA expression of DEC1/2 in BC patients and cell lines were taken from the Oncomine and Cancer Cell Line Encyclopedia database. The prognostic impacts of DEC1/2 were mined from the bc-GenExMiner and Kaplan–Meier plotter database. The impact of DEC1/2 genomic alterations on patient survival was calculated by cBioPortal. DEC2 protein expressions were confirmed by Western blotting (WB) in 10 pairs of BC samples. In addition, DEC2 sgRNA was constructed to confirm its affection on cell viability, invasion, and colony formation. RESULTS: The DEC1 and DEC2 mRNA levels are both lower in BC tissues than normal tissues. DEC1/2 expression was high in progesterone receptor (PR) positive BC patients (P = 0.0023), but low in human epidermal growth factor receptor 2 (HER2) positive patients (P < 0.0001). Lower DEC2 mRNA level has significant association with more aggressive pathogenic grade (P < 0.0001) and worse overall survival (OS) of BC patients (P = 5.2 × 10(−6)). Subgroup analysis showed that low DEC2 level was correlated with worse OS in estrogen receptor (ER) positive BC (P = 0.008). DEC2 (P = 0.00029) alteration was significantly correlated with worse OS in BC patients. WB results also confirmed the lower DEC2 protein levels in BC samples than their paired normal tissues. And, DEC2 silencing by sgRNA resulted in a significant increasing in cell viability, invasion, and colony formation. CONCLUSION: DEC2 might serve as a tumor suppressor, and its disfunction may involve in the tumorigenesis and indicate bad clinical outcomes in BC patients.
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spelling pubmed-75694332020-10-22 DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma Fang, Wentong Li, Qian Wang, Min Zheng, Mingjie Xu, Huirong Dis Markers Research Article BACKGROUND: Identification of new biomarkers can facilitate the development of effective therapeutic strategies in breast cancer (BC). Data from previous studies have revealed that differentiated embryonic chondrocyte gene (DEC) 1 and DEC2 might involve in the progression of various cancer types. We explored the expression profiles and function of DEC1/2 in BC patients in this study. METHODS: The mRNA expression of DEC1/2 in BC patients and cell lines were taken from the Oncomine and Cancer Cell Line Encyclopedia database. The prognostic impacts of DEC1/2 were mined from the bc-GenExMiner and Kaplan–Meier plotter database. The impact of DEC1/2 genomic alterations on patient survival was calculated by cBioPortal. DEC2 protein expressions were confirmed by Western blotting (WB) in 10 pairs of BC samples. In addition, DEC2 sgRNA was constructed to confirm its affection on cell viability, invasion, and colony formation. RESULTS: The DEC1 and DEC2 mRNA levels are both lower in BC tissues than normal tissues. DEC1/2 expression was high in progesterone receptor (PR) positive BC patients (P = 0.0023), but low in human epidermal growth factor receptor 2 (HER2) positive patients (P < 0.0001). Lower DEC2 mRNA level has significant association with more aggressive pathogenic grade (P < 0.0001) and worse overall survival (OS) of BC patients (P = 5.2 × 10(−6)). Subgroup analysis showed that low DEC2 level was correlated with worse OS in estrogen receptor (ER) positive BC (P = 0.008). DEC2 (P = 0.00029) alteration was significantly correlated with worse OS in BC patients. WB results also confirmed the lower DEC2 protein levels in BC samples than their paired normal tissues. And, DEC2 silencing by sgRNA resulted in a significant increasing in cell viability, invasion, and colony formation. CONCLUSION: DEC2 might serve as a tumor suppressor, and its disfunction may involve in the tumorigenesis and indicate bad clinical outcomes in BC patients. Hindawi 2020-10-10 /pmc/articles/PMC7569433/ /pubmed/33101542 http://dx.doi.org/10.1155/2020/6053154 Text en Copyright © 2020 Wentong Fang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fang, Wentong
Li, Qian
Wang, Min
Zheng, Mingjie
Xu, Huirong
DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma
title DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma
title_full DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma
title_fullStr DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma
title_full_unstemmed DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma
title_short DEC2 Serves as Potential Tumor Suppressor in Breast Carcinoma
title_sort dec2 serves as potential tumor suppressor in breast carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569433/
https://www.ncbi.nlm.nih.gov/pubmed/33101542
http://dx.doi.org/10.1155/2020/6053154
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