Cargando…
Electron Beam-Induced Transformation in High-Density Amorphous Ices
[Image: see text] Amorphous ice is commonly used as a noncrystalline matrix for protecting sensitive biological samples in cryogenic electron microscopy (cryo-EM). The amorphization process of water is complex, and at least two amorphous states of different densities are known to exist, high- and lo...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569672/ https://www.ncbi.nlm.nih.gov/pubmed/32997503 http://dx.doi.org/10.1021/acs.jpcb.0c08232 |
_version_ | 1783596778154622976 |
---|---|
author | Xu, Hongyi Ångström, Jonas Eklund, Tobias Amann-Winkel, Katrin |
author_facet | Xu, Hongyi Ångström, Jonas Eklund, Tobias Amann-Winkel, Katrin |
author_sort | Xu, Hongyi |
collection | PubMed |
description | [Image: see text] Amorphous ice is commonly used as a noncrystalline matrix for protecting sensitive biological samples in cryogenic electron microscopy (cryo-EM). The amorphization process of water is complex, and at least two amorphous states of different densities are known to exist, high- and low-density amorphous ices (HDA and LDA). These forms are considered to be the counterparts of two distinct liquid states, namely, high- and low-density liquid water. Herein, we investigate the HDA to LDA transition using electron diffraction and cryo-EM. The observed phase transition is induced by the impact of electrons, and we discuss two different mechanisms, namely, local heating and beam-induced motion of water molecules. The temperature increase is estimated by comparison with X-ray scattering experiments on identically prepared samples. Our results suggest that HDA, under the conditions used in our cryo-EM measurements, is locally heated above its glass-transition temperature. |
format | Online Article Text |
id | pubmed-7569672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-75696722020-10-20 Electron Beam-Induced Transformation in High-Density Amorphous Ices Xu, Hongyi Ångström, Jonas Eklund, Tobias Amann-Winkel, Katrin J Phys Chem B [Image: see text] Amorphous ice is commonly used as a noncrystalline matrix for protecting sensitive biological samples in cryogenic electron microscopy (cryo-EM). The amorphization process of water is complex, and at least two amorphous states of different densities are known to exist, high- and low-density amorphous ices (HDA and LDA). These forms are considered to be the counterparts of two distinct liquid states, namely, high- and low-density liquid water. Herein, we investigate the HDA to LDA transition using electron diffraction and cryo-EM. The observed phase transition is induced by the impact of electrons, and we discuss two different mechanisms, namely, local heating and beam-induced motion of water molecules. The temperature increase is estimated by comparison with X-ray scattering experiments on identically prepared samples. Our results suggest that HDA, under the conditions used in our cryo-EM measurements, is locally heated above its glass-transition temperature. American Chemical Society 2020-09-30 2020-10-15 /pmc/articles/PMC7569672/ /pubmed/32997503 http://dx.doi.org/10.1021/acs.jpcb.0c08232 Text en This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Xu, Hongyi Ångström, Jonas Eklund, Tobias Amann-Winkel, Katrin Electron Beam-Induced Transformation in High-Density Amorphous Ices |
title | Electron Beam-Induced Transformation in High-Density
Amorphous Ices |
title_full | Electron Beam-Induced Transformation in High-Density
Amorphous Ices |
title_fullStr | Electron Beam-Induced Transformation in High-Density
Amorphous Ices |
title_full_unstemmed | Electron Beam-Induced Transformation in High-Density
Amorphous Ices |
title_short | Electron Beam-Induced Transformation in High-Density
Amorphous Ices |
title_sort | electron beam-induced transformation in high-density
amorphous ices |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569672/ https://www.ncbi.nlm.nih.gov/pubmed/32997503 http://dx.doi.org/10.1021/acs.jpcb.0c08232 |
work_keys_str_mv | AT xuhongyi electronbeaminducedtransformationinhighdensityamorphousices AT angstromjonas electronbeaminducedtransformationinhighdensityamorphousices AT eklundtobias electronbeaminducedtransformationinhighdensityamorphousices AT amannwinkelkatrin electronbeaminducedtransformationinhighdensityamorphousices |