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Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
(1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem ce...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569883/ https://www.ncbi.nlm.nih.gov/pubmed/32971891 http://dx.doi.org/10.3390/polym12092163 |
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author | Liu, Xing Baldit, Adrien de Brosses, Emilie Velard, Frédéric Cauchois, Ghislaine Chen, Yun Wang, Xiong de Isla, Natalia Laurent, Cédric |
author_facet | Liu, Xing Baldit, Adrien de Brosses, Emilie Velard, Frédéric Cauchois, Ghislaine Chen, Yun Wang, Xiong de Isla, Natalia Laurent, Cédric |
author_sort | Liu, Xing |
collection | PubMed |
description | (1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering. |
format | Online Article Text |
id | pubmed-7569883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75698832020-10-29 Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering Liu, Xing Baldit, Adrien de Brosses, Emilie Velard, Frédéric Cauchois, Ghislaine Chen, Yun Wang, Xiong de Isla, Natalia Laurent, Cédric Polymers (Basel) Article (1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering. MDPI 2020-09-22 /pmc/articles/PMC7569883/ /pubmed/32971891 http://dx.doi.org/10.3390/polym12092163 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Xing Baldit, Adrien de Brosses, Emilie Velard, Frédéric Cauchois, Ghislaine Chen, Yun Wang, Xiong de Isla, Natalia Laurent, Cédric Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering |
title | Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering |
title_full | Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering |
title_fullStr | Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering |
title_full_unstemmed | Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering |
title_short | Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering |
title_sort | characterization of bone marrow and wharton’s jelly mesenchymal stromal cells response on multilayer braided silk and silk/plcl scaffolds for ligament tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569883/ https://www.ncbi.nlm.nih.gov/pubmed/32971891 http://dx.doi.org/10.3390/polym12092163 |
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