Cargando…

Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering

(1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xing, Baldit, Adrien, de Brosses, Emilie, Velard, Frédéric, Cauchois, Ghislaine, Chen, Yun, Wang, Xiong, de Isla, Natalia, Laurent, Cédric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569883/
https://www.ncbi.nlm.nih.gov/pubmed/32971891
http://dx.doi.org/10.3390/polym12092163
_version_ 1783596821735538688
author Liu, Xing
Baldit, Adrien
de Brosses, Emilie
Velard, Frédéric
Cauchois, Ghislaine
Chen, Yun
Wang, Xiong
de Isla, Natalia
Laurent, Cédric
author_facet Liu, Xing
Baldit, Adrien
de Brosses, Emilie
Velard, Frédéric
Cauchois, Ghislaine
Chen, Yun
Wang, Xiong
de Isla, Natalia
Laurent, Cédric
author_sort Liu, Xing
collection PubMed
description (1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering.
format Online
Article
Text
id pubmed-7569883
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75698832020-10-29 Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering Liu, Xing Baldit, Adrien de Brosses, Emilie Velard, Frédéric Cauchois, Ghislaine Chen, Yun Wang, Xiong de Isla, Natalia Laurent, Cédric Polymers (Basel) Article (1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering. MDPI 2020-09-22 /pmc/articles/PMC7569883/ /pubmed/32971891 http://dx.doi.org/10.3390/polym12092163 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xing
Baldit, Adrien
de Brosses, Emilie
Velard, Frédéric
Cauchois, Ghislaine
Chen, Yun
Wang, Xiong
de Isla, Natalia
Laurent, Cédric
Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
title Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
title_full Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
title_fullStr Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
title_full_unstemmed Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
title_short Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering
title_sort characterization of bone marrow and wharton’s jelly mesenchymal stromal cells response on multilayer braided silk and silk/plcl scaffolds for ligament tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569883/
https://www.ncbi.nlm.nih.gov/pubmed/32971891
http://dx.doi.org/10.3390/polym12092163
work_keys_str_mv AT liuxing characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT balditadrien characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT debrossesemilie characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT velardfrederic characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT cauchoisghislaine characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT chenyun characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT wangxiong characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT deislanatalia characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering
AT laurentcedric characterizationofbonemarrowandwhartonsjellymesenchymalstromalcellsresponseonmultilayerbraidedsilkandsilkplclscaffoldsforligamenttissueengineering