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Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting and death, which has a great impact on the yield and fruit quality. In this study, genetic analysis and quantitative trait loci (Q...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570005/ https://www.ncbi.nlm.nih.gov/pubmed/32906664 http://dx.doi.org/10.3390/plants9091155 |
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author | Dong, Shaoyun Zhang, Song Wei, Shuang Liu, Yanyan Li, Caixia Bo, Kailiang Miao, Han Gu, Xingfang Zhang, Shengping |
author_facet | Dong, Shaoyun Zhang, Song Wei, Shuang Liu, Yanyan Li, Caixia Bo, Kailiang Miao, Han Gu, Xingfang Zhang, Shengping |
author_sort | Dong, Shaoyun |
collection | PubMed |
description | High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting and death, which has a great impact on the yield and fruit quality. In this study, genetic analysis and quantitative trait loci (QTL) mapping for thermotolerance in cucumber seedlings was investigated using a recombinant inbred line (RILs; HR) population and a doubled haploid (DH; HP) population derived from two parental lines ‘65G’ (heat-sensitive) and ‘02245′ (heat-tolerant). Inheritance analysis suggested that both short-term extreme and long-term mild thermotolerance in cucumber seedlings were determined by multiple genes. Six QTLs for heat tolerance including qHT3.1, qHT3.2, qHT3.3, qHT4.1, qHT4.2, and qHT6.1 were detected. Among them, the major QTL, qHT3.2, was repeatedly detected for three times in HR and HP at different environments, explained 28.3% of the phenotypic variability. The 481.2 kb region harbored 79 genes, nine of which might involve in heat stress response. This study provides a basis for further identifying thermotolerant genes and helps understanding the molecular mechanism underlying thermotolerance in cucumber seedlings. |
format | Online Article Text |
id | pubmed-7570005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75700052020-10-29 Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings Dong, Shaoyun Zhang, Song Wei, Shuang Liu, Yanyan Li, Caixia Bo, Kailiang Miao, Han Gu, Xingfang Zhang, Shengping Plants (Basel) Article High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting and death, which has a great impact on the yield and fruit quality. In this study, genetic analysis and quantitative trait loci (QTL) mapping for thermotolerance in cucumber seedlings was investigated using a recombinant inbred line (RILs; HR) population and a doubled haploid (DH; HP) population derived from two parental lines ‘65G’ (heat-sensitive) and ‘02245′ (heat-tolerant). Inheritance analysis suggested that both short-term extreme and long-term mild thermotolerance in cucumber seedlings were determined by multiple genes. Six QTLs for heat tolerance including qHT3.1, qHT3.2, qHT3.3, qHT4.1, qHT4.2, and qHT6.1 were detected. Among them, the major QTL, qHT3.2, was repeatedly detected for three times in HR and HP at different environments, explained 28.3% of the phenotypic variability. The 481.2 kb region harbored 79 genes, nine of which might involve in heat stress response. This study provides a basis for further identifying thermotolerant genes and helps understanding the molecular mechanism underlying thermotolerance in cucumber seedlings. MDPI 2020-09-07 /pmc/articles/PMC7570005/ /pubmed/32906664 http://dx.doi.org/10.3390/plants9091155 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dong, Shaoyun Zhang, Song Wei, Shuang Liu, Yanyan Li, Caixia Bo, Kailiang Miao, Han Gu, Xingfang Zhang, Shengping Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings |
title | Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings |
title_full | Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings |
title_fullStr | Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings |
title_full_unstemmed | Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings |
title_short | Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings |
title_sort | identification of quantitative trait loci controlling high-temperature tolerance in cucumber (cucumis sativus l.) seedlings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570005/ https://www.ncbi.nlm.nih.gov/pubmed/32906664 http://dx.doi.org/10.3390/plants9091155 |
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