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Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings

High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting and death, which has a great impact on the yield and fruit quality. In this study, genetic analysis and quantitative trait loci (Q...

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Autores principales: Dong, Shaoyun, Zhang, Song, Wei, Shuang, Liu, Yanyan, Li, Caixia, Bo, Kailiang, Miao, Han, Gu, Xingfang, Zhang, Shengping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570005/
https://www.ncbi.nlm.nih.gov/pubmed/32906664
http://dx.doi.org/10.3390/plants9091155
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author Dong, Shaoyun
Zhang, Song
Wei, Shuang
Liu, Yanyan
Li, Caixia
Bo, Kailiang
Miao, Han
Gu, Xingfang
Zhang, Shengping
author_facet Dong, Shaoyun
Zhang, Song
Wei, Shuang
Liu, Yanyan
Li, Caixia
Bo, Kailiang
Miao, Han
Gu, Xingfang
Zhang, Shengping
author_sort Dong, Shaoyun
collection PubMed
description High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting and death, which has a great impact on the yield and fruit quality. In this study, genetic analysis and quantitative trait loci (QTL) mapping for thermotolerance in cucumber seedlings was investigated using a recombinant inbred line (RILs; HR) population and a doubled haploid (DH; HP) population derived from two parental lines ‘65G’ (heat-sensitive) and ‘02245′ (heat-tolerant). Inheritance analysis suggested that both short-term extreme and long-term mild thermotolerance in cucumber seedlings were determined by multiple genes. Six QTLs for heat tolerance including qHT3.1, qHT3.2, qHT3.3, qHT4.1, qHT4.2, and qHT6.1 were detected. Among them, the major QTL, qHT3.2, was repeatedly detected for three times in HR and HP at different environments, explained 28.3% of the phenotypic variability. The 481.2 kb region harbored 79 genes, nine of which might involve in heat stress response. This study provides a basis for further identifying thermotolerant genes and helps understanding the molecular mechanism underlying thermotolerance in cucumber seedlings.
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spelling pubmed-75700052020-10-29 Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings Dong, Shaoyun Zhang, Song Wei, Shuang Liu, Yanyan Li, Caixia Bo, Kailiang Miao, Han Gu, Xingfang Zhang, Shengping Plants (Basel) Article High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting and death, which has a great impact on the yield and fruit quality. In this study, genetic analysis and quantitative trait loci (QTL) mapping for thermotolerance in cucumber seedlings was investigated using a recombinant inbred line (RILs; HR) population and a doubled haploid (DH; HP) population derived from two parental lines ‘65G’ (heat-sensitive) and ‘02245′ (heat-tolerant). Inheritance analysis suggested that both short-term extreme and long-term mild thermotolerance in cucumber seedlings were determined by multiple genes. Six QTLs for heat tolerance including qHT3.1, qHT3.2, qHT3.3, qHT4.1, qHT4.2, and qHT6.1 were detected. Among them, the major QTL, qHT3.2, was repeatedly detected for three times in HR and HP at different environments, explained 28.3% of the phenotypic variability. The 481.2 kb region harbored 79 genes, nine of which might involve in heat stress response. This study provides a basis for further identifying thermotolerant genes and helps understanding the molecular mechanism underlying thermotolerance in cucumber seedlings. MDPI 2020-09-07 /pmc/articles/PMC7570005/ /pubmed/32906664 http://dx.doi.org/10.3390/plants9091155 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dong, Shaoyun
Zhang, Song
Wei, Shuang
Liu, Yanyan
Li, Caixia
Bo, Kailiang
Miao, Han
Gu, Xingfang
Zhang, Shengping
Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
title Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
title_full Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
title_fullStr Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
title_full_unstemmed Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
title_short Identification of Quantitative Trait Loci Controlling High-Temperature Tolerance in Cucumber (Cucumis sativus L.) Seedlings
title_sort identification of quantitative trait loci controlling high-temperature tolerance in cucumber (cucumis sativus l.) seedlings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570005/
https://www.ncbi.nlm.nih.gov/pubmed/32906664
http://dx.doi.org/10.3390/plants9091155
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