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Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid

Polyploidization is a universal phenomenon in plants and plays a crucial role in evolution. In this study, the transcriptomes of developing seeds of a synthetic Brassica hexaploid and its parents (B. rapa and B. carinata) were analyzed to find the gene expression changes in hexaploid seeds. There we...

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Autores principales: Liu, Zhengyi, Wang, Ruihua, Wang, Jianbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570109/
https://www.ncbi.nlm.nih.gov/pubmed/32899324
http://dx.doi.org/10.3390/plants9091141
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author Liu, Zhengyi
Wang, Ruihua
Wang, Jianbo
author_facet Liu, Zhengyi
Wang, Ruihua
Wang, Jianbo
author_sort Liu, Zhengyi
collection PubMed
description Polyploidization is a universal phenomenon in plants and plays a crucial role in evolution. In this study, the transcriptomes of developing seeds of a synthetic Brassica hexaploid and its parents (B. rapa and B. carinata) were analyzed to find the gene expression changes in hexaploid seeds. There were 3166 and 3893 DEGs between the Brassica hexaploid and its parents at the full-size stage and mature stage, respectively, most of which were upregulated in hexaploid seeds compared to its parents. At the mature stage, the hexaploid seeds showed a greater difference from its parents. These DEGs had a wide range of functions, which may account for the physiological and morphological differences between the Brassica hexaploid and its parents. The KEGG pathway analysis revealed that hexaploid seeds had higher levels of expression of genes involved in metabolic pathways, RNA transport and biosynthesis of secondary metabolites, and the expression levels in the photosynthesis-related pathways were significantly higher than those in B. rapa. Transgressive expression was the main non-additive expression pattern of the Brassica hexaploid. The gene expression difference between the Brassica hexaploid and its paternal parent was more significant than that with its maternal parent, which may be due in part to the cytoplasmic and maternal effects. Moreover, transcription factor genes, such as G2-like, MYB and mTERF, were highly expressed in hexaploid seeds, possibly promoting their resistance to stress. Our results may provide valuable insights into the adaptation mechanisms of polyploid plants.
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spelling pubmed-75701092020-10-28 Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid Liu, Zhengyi Wang, Ruihua Wang, Jianbo Plants (Basel) Article Polyploidization is a universal phenomenon in plants and plays a crucial role in evolution. In this study, the transcriptomes of developing seeds of a synthetic Brassica hexaploid and its parents (B. rapa and B. carinata) were analyzed to find the gene expression changes in hexaploid seeds. There were 3166 and 3893 DEGs between the Brassica hexaploid and its parents at the full-size stage and mature stage, respectively, most of which were upregulated in hexaploid seeds compared to its parents. At the mature stage, the hexaploid seeds showed a greater difference from its parents. These DEGs had a wide range of functions, which may account for the physiological and morphological differences between the Brassica hexaploid and its parents. The KEGG pathway analysis revealed that hexaploid seeds had higher levels of expression of genes involved in metabolic pathways, RNA transport and biosynthesis of secondary metabolites, and the expression levels in the photosynthesis-related pathways were significantly higher than those in B. rapa. Transgressive expression was the main non-additive expression pattern of the Brassica hexaploid. The gene expression difference between the Brassica hexaploid and its paternal parent was more significant than that with its maternal parent, which may be due in part to the cytoplasmic and maternal effects. Moreover, transcription factor genes, such as G2-like, MYB and mTERF, were highly expressed in hexaploid seeds, possibly promoting their resistance to stress. Our results may provide valuable insights into the adaptation mechanisms of polyploid plants. MDPI 2020-09-03 /pmc/articles/PMC7570109/ /pubmed/32899324 http://dx.doi.org/10.3390/plants9091141 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhengyi
Wang, Ruihua
Wang, Jianbo
Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid
title Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid
title_full Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid
title_fullStr Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid
title_full_unstemmed Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid
title_short Comprehensive Transcriptomic Analysis for Developing Seeds of a Synthetic Brassica Hexaploid
title_sort comprehensive transcriptomic analysis for developing seeds of a synthetic brassica hexaploid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570109/
https://www.ncbi.nlm.nih.gov/pubmed/32899324
http://dx.doi.org/10.3390/plants9091141
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