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Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores

Bracken fern (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) has long been grown industrially in South Korea. Conventional propagation methods, including planting rhizomes and in vitro seedling culture, are labor intensive and expensive, and thus not commercially suitable. We aim...

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Autores principales: Jang, Bo Kook, Cho, Ju Sung, Lee, Cheol Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570132/
https://www.ncbi.nlm.nih.gov/pubmed/32842585
http://dx.doi.org/10.3390/plants9091079
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author Jang, Bo Kook
Cho, Ju Sung
Lee, Cheol Hee
author_facet Jang, Bo Kook
Cho, Ju Sung
Lee, Cheol Hee
author_sort Jang, Bo Kook
collection PubMed
description Bracken fern (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) has long been grown industrially in South Korea. Conventional propagation methods, including planting rhizomes and in vitro seedling culture, are labor intensive and expensive, and thus not commercially suitable. We aimed to develop a system to produce synthetic seeds using fern spores (SFS). Synthetic seeds were prepared by mixing bracken spores and alginate matrix. Spore germination and gametophyte and sporophyte growth and development from SFS proceeded normally. Spore density affected gametophyte and sporophyte numbers. SFS prepared using cold (4 °C) long-term storage spores (even 7-year-old spores) could effectively form sporophytes. The highest germination was observed at 25 °C. Soaking-treated SFS successfully formed sporophytes, even after 30 days of storage at 4 °C; indeed, sporophytes formed even after five days of storage at 25 °C during transport conditions. SFS were sown in plug trays for commercial use. Young sporophytes grown from plug seedlings were greenhouse cultivated, and transplanting within eight weeks was effective for root growth and growing-point formation. Developing synthetic seeds is a feasible solution for facilitating efficient transport and the handling of small-sized fern spores; furthermore, this SFS technology provides the basis for fern seedling culture and fern spore industrialization.
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spelling pubmed-75701322020-10-28 Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores Jang, Bo Kook Cho, Ju Sung Lee, Cheol Hee Plants (Basel) Article Bracken fern (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) has long been grown industrially in South Korea. Conventional propagation methods, including planting rhizomes and in vitro seedling culture, are labor intensive and expensive, and thus not commercially suitable. We aimed to develop a system to produce synthetic seeds using fern spores (SFS). Synthetic seeds were prepared by mixing bracken spores and alginate matrix. Spore germination and gametophyte and sporophyte growth and development from SFS proceeded normally. Spore density affected gametophyte and sporophyte numbers. SFS prepared using cold (4 °C) long-term storage spores (even 7-year-old spores) could effectively form sporophytes. The highest germination was observed at 25 °C. Soaking-treated SFS successfully formed sporophytes, even after 30 days of storage at 4 °C; indeed, sporophytes formed even after five days of storage at 25 °C during transport conditions. SFS were sown in plug trays for commercial use. Young sporophytes grown from plug seedlings were greenhouse cultivated, and transplanting within eight weeks was effective for root growth and growing-point formation. Developing synthetic seeds is a feasible solution for facilitating efficient transport and the handling of small-sized fern spores; furthermore, this SFS technology provides the basis for fern seedling culture and fern spore industrialization. MDPI 2020-08-22 /pmc/articles/PMC7570132/ /pubmed/32842585 http://dx.doi.org/10.3390/plants9091079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jang, Bo Kook
Cho, Ju Sung
Lee, Cheol Hee
Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores
title Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores
title_full Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores
title_fullStr Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores
title_full_unstemmed Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores
title_short Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores
title_sort synthetic seed technology development and production studies for storage, transport, and industrialization of bracken spores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570132/
https://www.ncbi.nlm.nih.gov/pubmed/32842585
http://dx.doi.org/10.3390/plants9091079
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