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Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network

Polystyrene nanospheres (PNs) were embedded in bovine skin gelatin gels with a poly(N-isopropylacrylamide) (PNIPAAm) network, which were denoted as NGHHs, to generate thermoresponsive behavior. When 265 nm PNs were exploited to generate the pores, bovine skin gelatin extended to completely occupy th...

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Autores principales: Tsai, Fang-Chang, Huang, Chih-Feng, Chang, Chi-Jung, Lu, Chien-Hsing, Chen, Jem-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570140/
https://www.ncbi.nlm.nih.gov/pubmed/32971759
http://dx.doi.org/10.3390/polym12092156
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author Tsai, Fang-Chang
Huang, Chih-Feng
Chang, Chi-Jung
Lu, Chien-Hsing
Chen, Jem-Kun
author_facet Tsai, Fang-Chang
Huang, Chih-Feng
Chang, Chi-Jung
Lu, Chien-Hsing
Chen, Jem-Kun
author_sort Tsai, Fang-Chang
collection PubMed
description Polystyrene nanospheres (PNs) were embedded in bovine skin gelatin gels with a poly(N-isopropylacrylamide) (PNIPAAm) network, which were denoted as NGHHs, to generate thermoresponsive behavior. When 265 nm PNs were exploited to generate the pores, bovine skin gelatin extended to completely occupy the pores left by PNs below the lower critical solution temperature (LCST), forming a pore-less structure. Contrarily, above the LCST, the collapse of hydrogen bonding between bovine skin gelatin and PNIPAAm occurred, resulting in pores in the NGHH. The behavior of pore closing and opening below and above the LCST, respectively, indicates the excellent drug gating efficiency. Amoxicillin (AMX) was loaded into the NGHHs as smart antibiotic gating due to the pore closing and opening behavior. Accordingly, E. coli. and S. aureus were exploited to test the bacteria inhibition ratio (BIR) of the AMX-loaded NGHHs. BIRs of NGHH without pores were 48% to 46.7% at 25 and 37 °C, respectively, for E. coli during 12 h of incubation time. The BIRs of nanoporous NGHH could be enhanced from 61.5% to 90.4% providing a smart antibiotic gate of bovine skin gelatin gels against inflammation from infection or injury inflammation.
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spelling pubmed-75701402020-10-28 Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network Tsai, Fang-Chang Huang, Chih-Feng Chang, Chi-Jung Lu, Chien-Hsing Chen, Jem-Kun Polymers (Basel) Article Polystyrene nanospheres (PNs) were embedded in bovine skin gelatin gels with a poly(N-isopropylacrylamide) (PNIPAAm) network, which were denoted as NGHHs, to generate thermoresponsive behavior. When 265 nm PNs were exploited to generate the pores, bovine skin gelatin extended to completely occupy the pores left by PNs below the lower critical solution temperature (LCST), forming a pore-less structure. Contrarily, above the LCST, the collapse of hydrogen bonding between bovine skin gelatin and PNIPAAm occurred, resulting in pores in the NGHH. The behavior of pore closing and opening below and above the LCST, respectively, indicates the excellent drug gating efficiency. Amoxicillin (AMX) was loaded into the NGHHs as smart antibiotic gating due to the pore closing and opening behavior. Accordingly, E. coli. and S. aureus were exploited to test the bacteria inhibition ratio (BIR) of the AMX-loaded NGHHs. BIRs of NGHH without pores were 48% to 46.7% at 25 and 37 °C, respectively, for E. coli during 12 h of incubation time. The BIRs of nanoporous NGHH could be enhanced from 61.5% to 90.4% providing a smart antibiotic gate of bovine skin gelatin gels against inflammation from infection or injury inflammation. MDPI 2020-09-22 /pmc/articles/PMC7570140/ /pubmed/32971759 http://dx.doi.org/10.3390/polym12092156 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Fang-Chang
Huang, Chih-Feng
Chang, Chi-Jung
Lu, Chien-Hsing
Chen, Jem-Kun
Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network
title Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network
title_full Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network
title_fullStr Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network
title_full_unstemmed Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network
title_short Thermo-Tunable Pores and Antibiotic Gating Properties of Bovine Skin Gelatin Gels Prepared with Poly(n-isopropylacrylamide) Network
title_sort thermo-tunable pores and antibiotic gating properties of bovine skin gelatin gels prepared with poly(n-isopropylacrylamide) network
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570140/
https://www.ncbi.nlm.nih.gov/pubmed/32971759
http://dx.doi.org/10.3390/polym12092156
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