An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

Metabolic profiling based on (1)H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail Helix aspersa. Twenty metabolites were unambiguously identified by (1)H, 1D TOCSY, 2D J-resolved,...

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Autores principales: Vassilev, Nikolay G., Simova, Svetlana D., Dangalov, Miroslav, Velkova, Lyudmila, Atanasov, Venceslav, Dolashki, Aleksandar, Dolashka, Pavlinka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570211/
https://www.ncbi.nlm.nih.gov/pubmed/32887291
http://dx.doi.org/10.3390/metabo10090360
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author Vassilev, Nikolay G.
Simova, Svetlana D.
Dangalov, Miroslav
Velkova, Lyudmila
Atanasov, Venceslav
Dolashki, Aleksandar
Dolashka, Pavlinka
author_facet Vassilev, Nikolay G.
Simova, Svetlana D.
Dangalov, Miroslav
Velkova, Lyudmila
Atanasov, Venceslav
Dolashki, Aleksandar
Dolashka, Pavlinka
author_sort Vassilev, Nikolay G.
collection PubMed
description Metabolic profiling based on (1)H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail Helix aspersa. Twenty metabolites were unambiguously identified by (1)H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from Helix aspersa. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry.
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spelling pubmed-75702112020-10-28 An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus Vassilev, Nikolay G. Simova, Svetlana D. Dangalov, Miroslav Velkova, Lyudmila Atanasov, Venceslav Dolashki, Aleksandar Dolashka, Pavlinka Metabolites Article Metabolic profiling based on (1)H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail Helix aspersa. Twenty metabolites were unambiguously identified by (1)H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from Helix aspersa. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry. MDPI 2020-09-02 /pmc/articles/PMC7570211/ /pubmed/32887291 http://dx.doi.org/10.3390/metabo10090360 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vassilev, Nikolay G.
Simova, Svetlana D.
Dangalov, Miroslav
Velkova, Lyudmila
Atanasov, Venceslav
Dolashki, Aleksandar
Dolashka, Pavlinka
An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus
title An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus
title_full An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus
title_fullStr An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus
title_full_unstemmed An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus
title_short An (1)H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus
title_sort (1)h nmr- and ms-based study of metabolites profiling of garden snail helix aspersa mucus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570211/
https://www.ncbi.nlm.nih.gov/pubmed/32887291
http://dx.doi.org/10.3390/metabo10090360
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