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RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
Cyclophilin A/DIAGEOTROPICA (DGT) has been linked to auxin-regulated development in tomato and appears to affect multiple developmental pathways. Loss of DGT function results in a pleiotropic phenotype that is strongest in the roots, including shortened roots with no lateral branching. Here, we pres...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570327/ https://www.ncbi.nlm.nih.gov/pubmed/33123350 http://dx.doi.org/10.12688/f1000research.25750.1 |
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author | Ivanchenko, Maria G. Ozguc, Olivia R. Bollmann, Stephanie R. Fraser, Valerie N. Megraw, Molly |
author_facet | Ivanchenko, Maria G. Ozguc, Olivia R. Bollmann, Stephanie R. Fraser, Valerie N. Megraw, Molly |
author_sort | Ivanchenko, Maria G. |
collection | PubMed |
description | Cyclophilin A/DIAGEOTROPICA (DGT) has been linked to auxin-regulated development in tomato and appears to affect multiple developmental pathways. Loss of DGT function results in a pleiotropic phenotype that is strongest in the roots, including shortened roots with no lateral branching. Here, we present an RNA-Seq dataset comparing the gene expression profiles of wildtype (‘Ailsa Craig’) and dgt tissues from three spatially separated developmental stages of the tomato root tip, with three replicates for each tissue and genotype. We also identify differentially expressed genes, provide an initial comparison of genes affected in each genotype and tissue, and provide the pipeline used to analyze the data. Further analysis of this dataset can be used to gain insight into the effects of DGT on various root developmental pathways in tomato. |
format | Online Article Text |
id | pubmed-7570327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-75703272020-10-28 RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development Ivanchenko, Maria G. Ozguc, Olivia R. Bollmann, Stephanie R. Fraser, Valerie N. Megraw, Molly F1000Res Data Note Cyclophilin A/DIAGEOTROPICA (DGT) has been linked to auxin-regulated development in tomato and appears to affect multiple developmental pathways. Loss of DGT function results in a pleiotropic phenotype that is strongest in the roots, including shortened roots with no lateral branching. Here, we present an RNA-Seq dataset comparing the gene expression profiles of wildtype (‘Ailsa Craig’) and dgt tissues from three spatially separated developmental stages of the tomato root tip, with three replicates for each tissue and genotype. We also identify differentially expressed genes, provide an initial comparison of genes affected in each genotype and tissue, and provide the pipeline used to analyze the data. Further analysis of this dataset can be used to gain insight into the effects of DGT on various root developmental pathways in tomato. F1000 Research Limited 2020-09-28 /pmc/articles/PMC7570327/ /pubmed/33123350 http://dx.doi.org/10.12688/f1000research.25750.1 Text en Copyright: © 2020 Ivanchenko MG et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Data Note Ivanchenko, Maria G. Ozguc, Olivia R. Bollmann, Stephanie R. Fraser, Valerie N. Megraw, Molly RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development |
title | RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development |
title_full | RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development |
title_fullStr | RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development |
title_full_unstemmed | RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development |
title_short | RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development |
title_sort | rna-seq analysis of genes affected by cyclophilin a/diageotropica (dgt) in tomato root development |
topic | Data Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570327/ https://www.ncbi.nlm.nih.gov/pubmed/33123350 http://dx.doi.org/10.12688/f1000research.25750.1 |
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