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RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development

Cyclophilin A/DIAGEOTROPICA (DGT) has been linked to auxin-regulated development in tomato and appears to affect multiple developmental pathways. Loss of DGT function results in a pleiotropic phenotype that is strongest in the roots, including shortened roots with no lateral branching. Here, we pres...

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Autores principales: Ivanchenko, Maria G., Ozguc, Olivia R., Bollmann, Stephanie R., Fraser, Valerie N., Megraw, Molly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570327/
https://www.ncbi.nlm.nih.gov/pubmed/33123350
http://dx.doi.org/10.12688/f1000research.25750.1
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author Ivanchenko, Maria G.
Ozguc, Olivia R.
Bollmann, Stephanie R.
Fraser, Valerie N.
Megraw, Molly
author_facet Ivanchenko, Maria G.
Ozguc, Olivia R.
Bollmann, Stephanie R.
Fraser, Valerie N.
Megraw, Molly
author_sort Ivanchenko, Maria G.
collection PubMed
description Cyclophilin A/DIAGEOTROPICA (DGT) has been linked to auxin-regulated development in tomato and appears to affect multiple developmental pathways. Loss of DGT function results in a pleiotropic phenotype that is strongest in the roots, including shortened roots with no lateral branching. Here, we present an RNA-Seq dataset comparing the gene expression profiles of wildtype (‘Ailsa Craig’) and dgt tissues from three spatially separated developmental stages of the tomato root tip, with three replicates for each tissue and genotype. We also identify differentially expressed genes, provide an initial comparison of genes affected in each genotype and tissue, and provide the pipeline used to analyze the data. Further analysis of this dataset can be used to gain insight into the effects of DGT on various root developmental pathways in tomato.
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spelling pubmed-75703272020-10-28 RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development Ivanchenko, Maria G. Ozguc, Olivia R. Bollmann, Stephanie R. Fraser, Valerie N. Megraw, Molly F1000Res Data Note Cyclophilin A/DIAGEOTROPICA (DGT) has been linked to auxin-regulated development in tomato and appears to affect multiple developmental pathways. Loss of DGT function results in a pleiotropic phenotype that is strongest in the roots, including shortened roots with no lateral branching. Here, we present an RNA-Seq dataset comparing the gene expression profiles of wildtype (‘Ailsa Craig’) and dgt tissues from three spatially separated developmental stages of the tomato root tip, with three replicates for each tissue and genotype. We also identify differentially expressed genes, provide an initial comparison of genes affected in each genotype and tissue, and provide the pipeline used to analyze the data. Further analysis of this dataset can be used to gain insight into the effects of DGT on various root developmental pathways in tomato. F1000 Research Limited 2020-09-28 /pmc/articles/PMC7570327/ /pubmed/33123350 http://dx.doi.org/10.12688/f1000research.25750.1 Text en Copyright: © 2020 Ivanchenko MG et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Note
Ivanchenko, Maria G.
Ozguc, Olivia R.
Bollmann, Stephanie R.
Fraser, Valerie N.
Megraw, Molly
RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
title RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
title_full RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
title_fullStr RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
title_full_unstemmed RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
title_short RNA-Seq analysis of genes affected by Cyclophilin A/DIAGEOTROPICA (DGT) in tomato root development
title_sort rna-seq analysis of genes affected by cyclophilin a/diageotropica (dgt) in tomato root development
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570327/
https://www.ncbi.nlm.nih.gov/pubmed/33123350
http://dx.doi.org/10.12688/f1000research.25750.1
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